MBD2 Recombinant Monoclonal Antibody

說明書

中文名稱：

MBD2 Recombinant Monoclonal Antibody
貨號：

CSB-RA981891A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: HEK293 whole cell lysate(30μg)
All lanes: MBD2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 29 kDa
Exposure time: 3min

Overlay Peak curve showing Raji cells stained with CSB-RA981891A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
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靶點詳情

產品詳情

Uniprot No.：

Q9UBB5
基因名：

MBD2
別名：

Demethylase antibody; DMTase antibody; MBD 2 antibody; Mbd2 antibody; MBD2_HUMAN antibody; MBD2a antibody; Methyl CpG binding domain protein 2 antibody; Methyl CpG binding protein MBD2 antibody; Methyl-CpG-binding domain protein 2 antibody; Methyl-CpG-binding protein MBD2 antibody; NY CO 41 antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human MBD2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

3E5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
基因功能參考文獻：
1. Suggest that reduced MBD2 expression could contribute to chronic airway inflammation in COPD. PMID: 29535511
2. knockdown of MBD2 by siRNA attenuated vancomycin-induced apoptosis, caspase activity, and the expression of BAX and cleaved caspase 3. PMID: 29022913
3. CpG and methylation-dependent DNA binding and dynamics of the MBD2 protein at the single-molecule level have been reported. PMID: 28637186
4. Results show that MBD2 mediates epigenetic silencing of Htra1. PMID: 27388476
5. MBD2 has a critical role in 'rewriting' the cancer methylome at specific regulatory regions. PMID: 27593931
6. MBD2 expression was elevated in HCC tissue, which suggesting MBD2 as a candidate prognostic marker of HCC. PMID: 27315121
7. downregulation of miR-221 inhibits cell migration and invasion at least partially through targeting MBD2 in the human OSCC cell line UM1. PMID: 26788506
8. Specifically, methyl-CpG-binding domain protein 2 (MBD2) is revealed to be recruited to DNA damage sites after laser microirradiation, which was mediated through MBD domain and MBD2 C-terminus. PMID: 26827827
9. MBD2 targets short interspersed nuclear elements, but does not exclude RNA Polymerase III. PMID: 25798578
10. The dynamics of MBD2 deposition across methylated DNA regions was associated with the oncogenic transformation of human mammary cells. PMID: 26007656
11. Data suggest that MBD2 binds primarily at highly methylated regions, with a strong preference for CpG islands and highlights that MBD2 binding sites display increased methylation in primary breast cancer tissues as compared to normal mammary cells. PMID: 24927503
12. Biophysical analyses show that the MBD2IDR is an intrinsically disordered region (IDR). However, despite this inherent disorder, MBD2IDR increases the overall binding affinity of MBD2 for methylated DNA. PMID: 25753662
13. This study investigates the genetic association between methyl-CpG-binding domain (MBD) gene polymorphisms and schizophrenia. PMID: 24849540
14. alternatively spliced isoforms support pluripotency of stem cells PMID: 24813856
15. The methylated-DNA binding protein MBD2 enhances NGFI-A (egr-1)-mediated transcriptional activation of the glucocorticoid receptor. PMID: 25135974
16. These data point to a potential new approach in targeting the DNA methylation machinery by combination of MBD2 and DNMT inhibitors. PMID: 25178277
17. MBD2 seems to play a selective role in gene repression depending on the CpG content of the promoter region PMID: 23888954
18. Methylated DNA binding domain protein 2 (MBD2) coordinately silences gene expression through activation of the microRNA hsa-mir-496 promoter in breast cancer cell line. PMID: 24204564
19. reduced mRNA expression of MBD2 and MBD3 is implicated in gastric carcinogenesis. PMID: 24338710
20. global DNA hypomethylation is one of the possible epigenetic variations associated with the complex etiology of TOF and significantly correlates with the aberrant expression of MBD2 mRNA in patients with TOF. PMID: 23820632
21. MBD2 binds a significant fraction of the hypomethylated genes. PMID: 23955541
22. Abnormal expression levels of DNA (cytosine-5-)-methyltransferase 1 and MBD2 mRNA may be important causes of the global hypomethylation observed in CD4+T cells in systemic lupus erythematosus. PMID: 23127209
23. In metastatic colorectal cancer cells, reduced levels of miR-221* and miR-224 increase levels of MBD2, thereby decreasing expression of the metastasis suppressor maspin. PMID: 23770133
24. MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes. PMID: 23361464
25. These factors lead to a binding affinity hierarchy of p66alpha for the different MBD2 homologues (MBD2 approximately MBD3 > MBD3L1 approximately MBD3L2). PMID: 23239876
26. repressive functions of MBD2-containing NuRD complexes are dependent on cooperative interactions between the major domains of CHD4 with histones and DNA and on binding of methylated DNA by MBD2 PMID: 23071088
27. MiR-373 behaves as a direct transcriptional target and negative regulator of MBD2 activity through a feedback loop of CpG island methylation in hilar cholangiocarcinoma PMID: 22876037
28. The association between MBD2 binding and transcriptional repression weakened as the distance between binding site and TSS increased, suggesting that MBD2 represses transcriptional initiation PMID: 22048253
29. A two-stage association study identifies methyl-CpG-binding domain protein 2 gene polymorphisms as candidates for breast cancer susceptibility. PMID: 22258532
30. These results show a role for MBD2 in cancer progression and provide support for the prospect of targeting MBD2 therapeutically in aggressive breast cancers. PMID: 21693597
31. MBD2 overexpression during gliomagenesis may drive tumor growth by suppressing the antiangiogenic activity of a key tumor BAI1. PMID: 21724586
32. human epsilon-globin gene is subject to multilayered silencing mediated in part by MBD2 PMID: 21296012
33. In hilar cholangiocarcinoma, down-expression of miR-373 leads to increase of MBD2, which in turn suppresses the methylation-mediated gene such as RASSF1A. PMID: 21086164
34. This study demonstrated that patients with SLE had a significantly lower level of DNA methylation than the controls, and that expression of both DNMT1 and MBD2 mRNA was significantly increased in the SLE patients compared with controls. PMID: 21078759
35. Expression level of MBD2 is significantly lower in endometriotic lesions compared with disease-free controls. PMID: 21316665
36. miR-373 is a methylation-mediated gene and the implication of MBD2 in methylation-mediated suppression of miR-373 plays an important role in tumourigenesis and development in hilar cholangiocarcinoma. PMID: 21165562
37. The anti-TNFalpha biological agents do not seem to affect DNA methylation and mRNA expressions of DNMT1 and MBD2 in RA PMID: 20937307
38. MBD2 and ERalpha drive opposite effects on pS2 expression, which are associated with specific steady state levels of histone H3 acetylation and methylation marks PMID: 20300195
39. Methyl-CpG binding domain protein 2 represses transcription from hypermethylated pi-class glutathione S-transferase gene promoters in hepatocellular carcinoma cells PMID: 11960994
40. MBD2 protein activates CpG sites within the promoter region of reporter genes PMID: 12177048
41. interaction with two highly related p66 proteins PMID: 12183469
42. interacts with latency-associated nuclear antigen of Kaposi's Sarcoma-associated herpesvirus (KSHV)to tether KSHV to cell chromosomes PMID: 12388720
43. MBDin relieves MBD2 repression potential and reactivates transcription from methylated promoters PMID: 12588985
44. Results show that methyl-CpG binding domain protein 1 (MBD1) is expressed in tumor cells, but methyl-CpG binding domain protein 2 (MBD2) and methyl CpG binding protein 2 (MeCP2) are not. PMID: 12646234
45. MBD2a and RNA helicase A cooperatively enhanced CREB-dependent gene expression. PMID: 12665568
46. MBD2 has a role in the methylation-mediated inhibition of ribosomal RNA gene expression PMID: 14610093
47. Antisenses oligoDNA suppresses tumor growth in nude mice, a potential anticaner therapy approach. PMID: 14688029
48. MBD2 gene expression may be significant factor in tumorigenesis. PMID: 15112265
49. role of MBD3L1 as a methylation-dependent transcriptional repressor that may interchange with MBD3 as an MBD2-interacting component of the NuRD complex PMID: 15456747
50. MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing PMID: 15701600
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亞細胞定位：

Nucleus. Note=Nuclear, in discrete foci. Detected at replication foci in late S phase.
組織特異性：

Highly expressed in brain, heart, kidney, stomach, testis and placenta.
數據庫鏈接：

HGNC: 6917

OMIM: 603547

KEGG: hsa:8932

STRING: 9606.ENSP00000256429

UniGene: Hs.25674

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