MAS1L Recombinant Monoclonal Antibody
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中文名稱：MAS1L重組抗體
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貨號(hào)：CSB-RA971033A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue
All lanes: MAS1L antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 36-50 kDa -
Immunofluorescence staining of MCF-7 cell with CSB-RA971033A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of Hela cell with CSB-RA971033A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing Hela cells stained with CSB-RA971033A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA971033A0HU是靶向MAS1L蛋白的重組單克隆抗體，適用于多種實(shí)驗(yàn)場(chǎng)景的科研需求。該抗體針對(duì)MAS1L受體特異性設(shè)計(jì)，可精確識(shí)別其天然構(gòu)象表位，在多種驗(yàn)證實(shí)驗(yàn)中展現(xiàn)出優(yōu)異的性能：Western Blot（WB）推薦稀釋比例1:500-1:2000，可清晰檢測(cè)目標(biāo)蛋白條帶；免疫熒光（IF）和流式細(xì)胞術(shù)（FC）分別在1:50-1:200稀釋范圍內(nèi)呈現(xiàn)高信噪比染色效果，適用于亞細(xì)胞定位及表面標(biāo)志物分析。MAS1L作為G蛋白偶聯(lián)受體家族成員，參與調(diào)控細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)和組織穩(wěn)態(tài)，本產(chǎn)品可支持其在細(xì)胞功能研究（如增殖調(diào)控、分化機(jī)制探索）及疾病模型（如心血管或腫瘤相關(guān)基礎(chǔ)研究）中的表達(dá)分析。經(jīng)ELISA驗(yàn)證，該抗體具備高批次穩(wěn)定性與低交叉反應(yīng)性，可滿足復(fù)雜樣本的定量檢測(cè)需求。適用于體外培養(yǎng)細(xì)胞、組織裂解液等樣本類型，為受體功能研究、信號(hào)通路解析及分子互作實(shí)驗(yàn)提供可靠工具。
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Uniprot No.：
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基因名：
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別名：Mas-related G-protein coupled receptor MRG (MAS-R) (MAS1-like), MAS1L, MRG
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反應(yīng)種屬：Human, Rat
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免疫原：A synthesized peptide derived from human MAS1L
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：15B8
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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靶點(diǎn)詳情
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基因功能參考文獻(xiàn)：
- Mas, MrgD, and MRG mediated Ang IV-stimulated AA release that was highest for Mas. While Ang III activated Mas and MrgX2, Ang II stimulated AA release via Mas and MRG. PMID: 18636314
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亞細(xì)胞定位：Cell membrane; Multi-pass membrane protein.
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蛋白家族：G-protein coupled receptor 1 family, Mas subfamily
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數(shù)據(jù)庫鏈接：
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