MAP1LC3A Recombinant Monoclonal Antibody

說明書

中文名稱：

MAP1LC3A Recombinant Monoclonal Antibody
貨號：

CSB-RA616977A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA616977A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA616977A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela cell with CSB-RA616977A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing Hela cells stained with CSB-RA616977A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

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靶點詳情

產品詳情

Uniprot No.：

Q9H492
基因名：

MAP1LC3A
別名：

ATG8E antibody; Autophagy related protein LC3 A antibody; Autophagy related ubiquitin like modifier LC3 A antibody; Autophagy-related protein LC3 A antibody; Autophagy-related ubiquitin-like modifier LC3 A antibody; LC3 antibody; LC3A antibody; MAP1 light chain 3 like protein 1 antibody; MAP1 light chain 3-like protein 1 antibody; MAP1A/1B light chain 3 A antibody; MAP1A/MAP1B LC3 A antibody; MAP1A/MAP1B light chain 3 A antibody; MAP1ALC3 antibody; MAP1BLC3 antibody; Map1lc3a antibody; Microtubule associated proteins 1A/1B light chain 3 antibody; Microtubule-associated protein 1 light chain 3 alpha antibody; Microtubule-associated proteins 1A and 1B, light chain 3 antibody; Microtubule-associated proteins 1A/1B light chain 3A antibody; MLP3A_HUMAN antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human MAP1LC3A
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

11B9
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover.
基因功能參考文獻：
1. Expression and Clinical Significance of LC-3 and P62 in Non-small Cell Lung Cancer PMID: 29945702
2. High LC3 Expression is Associated with Cancer Progression and Chemo-Resistance in Esophageal Squamous Cell Carcinoma. PMID: 29022200
3. Data suggest that, in patients with diabetic kidney disease, urinary excretion of mRNAs for MAP1LC3A, WIPI2, and RB1CC1 is down-regulated as compared to healthy control subjects; these transcripts may serve as urinary autophagy biomarkers. (MAP1LC3A = microtubule associated protein 1 light chain 3; WIPI2 = WD repeat domain phosphoinositide-interacting protein 2; RB1CC1 = RB1 inducible coiled-coil 1) PMID: 28760651
4. Autophagy proteins were discovered on phagosomes engulfing foreign bacteria, leading to the proposal of LC3-associated phagocytosis (LAP). [review] PMID: 28755428
5. data raise important issues regarding the interpretation of LC3 lipidation data and the use of autophagy modulators PMID: 28296541
6. High LC3 expression is associated with endometriosis. PMID: 28348069
7. Co-expression of FKBP8 with LC3A profoundly induces Parkin-independent mitophagy. Strikingly, even when acting as a mitophagy receptor, FKBP8 avoids degradation by escaping from mitochondria. In summary, this study identifies novel roles for FKBP8 and LC3A, which act together to induce mitophagy. PMID: 28381481
8. we report that autophagy is associated with apoptosis processes, involving LC3 and TRIF-colocation in human HCC cells. Regulation of autophagy and the TLR3-TRIF pathway may be effective in the treatment of liver cancer. PMID: 27338037
9. The autophagy induced by 2-PCPA requires LC3-II processing machinery..2-PCPA treatment induces the change of global gene expression program, including a series of autophagy-related genes, such as SQSTM1/p62. Taken together, our data indicate that KDM1A/LSD1 inhibitors induce autophagy through affecting the expression of autophagy-related genes and in a BECN1-independent manner PMID: 28800922
10. LC3 and p62/SQSTM1 have roles in early-stage non-small cell lung cancer PMID: 27250032
11. Hotspot mutations in the arginine-rich stretch in MAP1LC3A resulting in reduced cleavage of MAP1LC3A by ATG4B both in vitro and in vivo, suggesting a functional implication of this gene mutation in cancer development. PMID: 27256984
12. analysis of soluble SQSTM1 complexes and soluble complexes formed between SQSTM1 oligomers and LC3 using a combination of fluorescence microscopy-based biophysical approaches in living cells PMID: 27442348
13. we found that ATG14 interacted with Ulk1 and LC3, and knock down of Ulk1 prevented the lipidation of LC3 and autophagy in HeLa-ATG14 cells. We also identified a phosphatidylethanolamine (PE) binding region in ATG14, and the addition of Ulk1 to Hela-ATG14 cells decreased the ATG14-PE interaction. PMID: 28069524
14. Legionella pneumophila RavZ extracts LC3-PE from the membrane before deconjugation. RavZ initially recognizes the LC3 molecule on membranes via its N-terminal LC3-interacting region (LIR) motif. PMID: 28395732
15. The results are consistent with a model in which RPIA suppresses autophagy and LC3 processing by modulation of redox signaling. PMID: 27328773
16. can act as a negative mediator in autophagy through stimulation of the AKT-MTORC1 pathway and direct interaction with LC3. PMID: 27540684
17. Data show that p62 interaction with LC3, leading to the delivery of p62 and its cargoes to the autophagosome. PMID: 28740232
18. LC3 puncta were still present in the granular layer of nonlesional psoriatic epidermis but at a much lower level compared with healthy adult epidermis, whereas in lesional psoriatic skin, LC3 was not expressed at all. PMID: 27021405
19. Results suggest that P. gingivalis internalization is not necessary for LC3 lipidation. PMID: 26452236
20. Although neither GABARAPs nor LC3s are required for autophagosome biogenesis, loss of all Atg8s yields smaller autophagosomes and a slowed initial rate of autophagosome formation. PMID: 27864321
21. Methylation silencing of MAP1LC3Av1 promotes gastric carcinogenesis. PMID: 28214334
22. Development of LC3/GABARAP sensors containing a LIR and a hydrophobic domain to monitor autophagy. PMID: 28320742
23. Aldo was revealed to induce autophagy, as indicated by the increased conversion from microtubuleassociated protein 1A/1Blight chain 3 (LC3)I to LC3II, the increased expression levels of autophagyrelated gene 7 (Atg7) and the increased degradation of p62, which was accompanied by MC proliferation PMID: 27748808
24. LC3 overexpression is associated with colorectal cancer. PMID: 27444698
25. these findings were reversed with the LC3I/II-shPTEN treatment. Therefore, the loss of PTEN may promote the development of primary resistance to trastuzumab in breast cancer via autophagy defects PMID: 26563373
26. autophagy of cytoplasmic bulk cargo was completely LC3 independent. PMID: 26237084
27. Results of our present study demonstrated that beclin 1 and LC3 immunoreactivity increased in carcinoma cells following exemestane treatment and that the status of pre-treatment stromal beclin 1 is associated with higher carcinoma cell proliferation and poor clinical and pathological responses to NAE PMID: 26984766
28. Consistent with the in vitro results, we observed that the expression of autophagy marker LC3 was increased. PMID: 26676801
29. Up-regulation of LC3 is associated with carcinogenesis and progression of Salivary Adenoid Cystic Carcinoma. PMID: 26350055
30. up-regulated expression during the monocyte-to-dendritic cell transition PMID: 26395023
31. LC3 is exploited by coxsackievirus in both autophagy-dependent and -independent manners in vivo PMID: 26090585
32. Autophagy status, as determined by combined LC3, Beclin 1 and p62 expression, might be independently associated with poor survival in patients with gastric cancer. PMID: 26722036
33. Western blot and immunofluorescence validated antibody specificity, knockdown efficiency and autophagy induction prior to fixation in formalin and embedding in paraffin. LC3B and p62 antibodies were validated on cell pellets PMID: 26150155
34. In colorectal cancer, differentiation degree and lymph node metastasis were associated with LC3 overexpression. LC3 expression was significantly higher in cancerous tissue than in adjacent tissue. PMID: 26097572
35. high nuclear HMGB1 levels and cytoplasmic HMGB1 expression predicted poor overall survival (OS) and disease-free survival (DFS). Meanwhile, high LC3 expression was associated with favorable prognosis. PMID: 26084608
36. These results demonstrate the essential role of BECN1 in the functional formation of autophagosomes, but not in LC3B lipidation. PMID: 25955014
37. The authors show that LC3 is recruited specifically around secreting Shigella flexneri bacteria that are still present in vacuoles formed during entry and cell-to-cell spread. PMID: 26015503
38. Downregulation of beta 3 -integrin with cRGD peptide resulted in enhanced LC3-II and Beclin-1 and decreased Bcl-2 expression. PMID: 25824726
39. These data add novel mechanistic insights into the regulation of noncanonical LC3 lipidation and its associated processes, including LC3-associated phagocytosis. PMID: 25484071
40. direct association with LC3 and p62 is promoted in osteoblasts by bioactive silica nanoparticles PMID: 24806912
41. Results showed that detergent-insoluble LC3-I and -II levels were significantly higher in multiple system atrophy brain tissue than those in controls PMID: 22959883
42. Clusterin facilitates stress-induced lipidation of LC3 and autophagosome biogenesis to enhance cancer cell survival. PMID: 25503391
43. This study aimed to explore autophagy-related protein light chain 3 (LC3)-II expression levels in thyroid diseases. PMID: 25661375
44. CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition PMID: 25483962
45. PLP2-TM interacts with the key autophagy regulators, LC3 and Beclin1, and promotes Beclin1 interaction with STING, the key regulator for antiviral IFN signaling, to negatively regulate antiviral innate immunity. PMID: 25311841
46. highlight the role that VAMP3 and VAMP7 play in selection of the pathways leading to generation of ultrastructurally different LC3 compartments PMID: 25046114
47. Patients with strong immunoreactivity to BECLIN 1 or LC3 had a significantly better overall survival (OS) than patients with negative to moderate immunoreactivity (p = 0.036 and 0.018, respectively). PMID: 25871810
48. A significant association of LC3 expression with disease progression including a large tumor size, higher stage, and worse prognosis of hepatocellular carcinoma. PMID: 25005847
49. high level of expression of BECLIN 1 and LC3 in tumours is well correlated with the overall survival of the patients. PMID: 25136588
50. Results show that acetylation-deacetylation cycle ensures that LC3 effectively redistributes in an activated form from nucleus to cytoplasm, where it plays a central role in autophagy. PMID: 25601754
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亞細胞定位：

Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome. Note=LC3-II binds to the autophagic membranes.
蛋白家族：

ATG8 family
組織特異性：

Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
數據庫鏈接：

HGNC: 6838

OMIM: 601242

KEGG: hsa:84557

STRING: 9606.ENSP00000363970

UniGene: Hs.632273

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