LPIN1 Recombinant Monoclonal Antibody

說明書

中文名稱：

LPIN1 Recombinant Monoclonal Antibody
貨號：

CSB-RA944336A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: A549 whole cell lysate(30μg)
All lanes: LPIN1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 99 kDa
Observed band size: 130 kDa
Exposure time：3min

IHC image of CSB-RA944336A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA944336A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q14693
基因名：

LPIN1
別名：

EC=3.1.3.4 antibody; KIAA0188 antibody; Lipin-1 antibody; Lpin1 antibody; LPIN1_HUMAN antibody; PAP1 antibody; Phosphatidate phosphatase LPIN1 antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human LPIN1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7C11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression. Is involved in adipocyte differentiation. Isoform 1 is recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.
基因功能參考文獻：
1. Normal human adipose tissue functions and differentiation has been found in rhabdomyolysis patients with biallelic LPIN1 inactivating mutations. PMID: 28986436
2. conclude that Lipin-1 can antagonize HSC activation through the inhibition of TGF-beta/SMAD signaling and that resveratrol may affect Lipin-1 gene induction and contribute to the inhibition of TGF-beta-mediated hepatic fibrogenesis PMID: 27345520
3. beta-TRCP depletion in HepG2 hepatocellular carcinoma cells resulted in increased Lipin1 protein abundance. PMID: 28049764
4. This study showed that LPIN1 knockdown blocks phospholipid synthesis and changes membrane lipid compositions, and that lipin-1 knockdown significantly inhibits tumor growth in vivo using an orthotopic xenograft breast mouse model. PMID: 28347999
5. The novel insights into the regulation of human Lipin-1 stability will be useful in planning further studies to elucidate its metabolic processes. PMID: 28483528
6. It seems that a new signaling axis, SIRT1-SFRS10-LPIN1 axis, acting in the pathogenesis of alcoholic fatty liver disease exists. PMID: 28467182
7. Consistent with these observations, LPIN1 levels were positively correlated with IRS1 expression in human breast cancer. Thus, our results indicate a mechanism by which IRS1 expression is increased in breast cancer, and LPIN1 may be a promising drug target for anticancer therapy PMID: 27729374
8. LPIN1 is upregulated in non-alcoholic fatty liver disease. Up-regulation of miR-122 can trigger the compensatory response of LPIN1 and CTDNEP1 in hepatosteatosis. PMID: 28287811
9. lipin-1-mediated downregulation of p21 is critical for the progress of keratinocyte differentiation PMID: 26658689
10. lipin-1 has a critical role in the regulation of macrophage inflammatory responses to modified-LDL PMID: 26288136
11. Results of our study suggest that rs2716610: C>T polymorphism of LPIN1 gene could have a protective effect against development of metabolic syndrome, while rs11693809: C>T might affect a glucose control in patients with MS. PMID: 26276647
12. Lipin-1 controls main cellular processes involved in cancer progression and that its targeting, alone or in combination with other treatments, could open new avenues in anticancer therapy. PMID: 25834103
13. Lipin1beta might play a role in the pathogenesis of insulin resistance in gestational diabetes mellitus PMID: 25974997
14. LPIN1 plays a functional role in lipid synthesis and storage, a role which is highly conserved from human to yeast. PMID: 25475986
15. the lipin-1 N-terminal domain is important for its catalytic activity, nuclear localization, and binding to PP-1cgamma PMID: 24558042
16. Lipin-1 deficiency is associated with lipid metabolism alterations resulting in massive rhabdomyolysis. PMID: 23928362
17. the SNP in the LPIN1 gene (rs10192566) had no effect on the trough rosiglitazone steady-state concentration. PMID: 23426382
18. Phosphorylation of lipin 1 and charge on the phosphatidic acid head group control its phosphatidic acid phosphatase activity and membrane association PMID: 23426360
19. Data suggests common single nucleotide polymorphisms within the LPIN1 region were associated with Type 2 diabetes and metabolic traits in the Chinese population. PMID: 22853689
20. LPIN1-related myolysis constitutes a major cause of early-onset rhabdomyolysis and occasionally in adults. Heterozygous LPIN1 mutations may cause mild muscular symptoms. No major defects of LPIN2 or LPIN3 genes were associated with muscle manifestations. PMID: 22481384
21. We conclude that direct control of lipin 1 transcription by HIF-1 is an important regulatory feature of lipid metabolism and its adaptation to hypoxia. PMID: 22467849
22. Lipin1 rs13412852 single nucleotide polymorphism is associated with the severity of liver damage and fibrosis progression in pediatric patients with histological NAFLD. PMID: 22157924
23. LPIN1 mutations should be considered in any child presenting with severe rhabdomyolysis. PMID: 22480698
24. Estrogen-related receptor gamma (ERRgamma) is a novel transcriptional regulator of phosphatidic acid phosphatase, LIPIN1, and inhibits hepatic insulin signaling PMID: 21911493
25. reduced expression of SFRS10, as observed in tissues from obese humans, alters LPIN1 splicing, induces lipogenesis, and therefore contributes to metabolic phenotypes associated with obesity. PMID: 21803291
26. Lpin1 expression in response to nutritional stress is controlled through the reactive oxygen species-ATM-p53 pathway. PMID: 22055193
27. The aim of present study was to investigate for the first time the association of lipin 1 gene polymorphisms with metabolic and hormonal profile in polycystic ovary syndrome patients and controls. PMID: 21448847
28. PPARG and LPIN1 gene polymorphisms role of these genes in lipid metabolism and pathogenesis of type 2 diabetes and metabolic syndrome PMID: 21263402
29. lipin-1gamma is the main lipin-1 isoform expressed in normal human brain, suggesting a specialized role in regulating brain lipid metabolism. PMID: 21616074
30. data suggest that lipin-1 associates with lipid droplets and regulates the activation of cytosolic group IVA phospholipase A(2)alpha in monocyte-derived macrophages PMID: 21478406
31. dullard shows specificity for the peptide corresponding to the insulin-dependent phosphorylation site (Ser106) of lipin PMID: 21413788
32. Data revealed that lipin 1 formed stable homo-oligomers with itself and hetero-oligomers with lipin 2/3. PMID: 20735359
33. Results showed a marked association between LPIN1 and PPARalpha gene expression both in subcutaneous and visceral adipose tissues and might suggest a possible role of LPIN1 in stress conditions that occur in chronic obesity and underlie insulin resistance PMID: 20101248
34. Data suggest that the high frequency of the intragenic LPIN1 deletion should provide a valuable criterion for fast diagnosis, prior to muscle biopsy. PMID: 20583302
35. the low-grade proinflammatory environment and the insulin resistance associated with obesity may contribute to downregulate LPIN1 in adipose tissue PMID: 20530740
36. LPIN1 rs33997857, rs6744682, and rs6708316 did not associate with type 2 diabetes, obesity, or related quantitative metabolic phenotypes in the Danish population examined. PMID: 20356931
37. the LPIN1 gene seemed not to be a major susceptibility gene for type 2 diabetes or related metabolic phenotypes in the Chinese population. PMID: 19543209
38. sumoylation facilitates the nuclear localization and transcriptional coactivator behavior of lipin-1alpha, which may act as a sumoylation-regulated transcriptional coactivator in brain PMID: 19753306
39. LPIN1 mutations are not commonly seen in patients with lipodystrophy who had no mutations in known disease genes. PMID: 12111372
40. effects of genetic variation in lipin levels on glucose homeostasis across species by analyzing lipin transcript levels in human and mouse adipose tissues PMID: 16357106
41. human lipin 1 is a Mg2+-dependent PA phosphatase, the penultimate enzyme in the pathway to synthesize triacylglyerol PMID: 16467296
42. These results suggest that increased adipogenesis and/or lipogenesis in subcutaneous fat, mediated by the LPIN1 gene, may prevent lipotoxicity in muscle, leading to improved insulin sensitivity PMID: 17003347
43. Body fat accumulation is a major regulator of human adipose LPIN1 expression and suggests a role of LPIN1 in human preadipocyte as well as mature adipocyte function. PMID: 17035674
44. insulin and epinephrine control lipin primarily by changing localization rather than intrinsic PAP activity PMID: 17105729
45. Lipin 1beta and overexpression of peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha are downregulated by obesity and obesity-related metabolic perturbations in human subjects. PMID: 17563064
46. Variants of the LPIN1 gene have significant effects in human metabolic traits and implicate lipin in the pathophysiology of the metabolic syndrome. PMID: 17940119
47. LPIN1 messenger RNA levels positively correlated with insulin sensitivity in all subjects with obesity, NIDDM, and HIV-associated lipodystrophy PMID: 17950103
48. Sequence variation in the LPIN1 gene contributes to variation in resting metabolic rate and obesity-related phenotypes potentially in an age-dependent manner. PMID: 18070763
49. Lpin1 glucocorticoid response element binds to the glucocorticoid receptor PMID: 18362392
50. Study provides further evidence that lipin 1 may play a role in blood pressure regulation, especially in men. PMID: 18437145
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相關疾?。?/div>
Myoglobinuria, acute recurrent, autosomal recessive (ARARM)
亞細胞定位：

Nucleus membrane. Cytoplasm, cytosol. Endoplasmic reticulum membrane.
蛋白家族：

Lipin family
組織特異性：

Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract.
數據庫鏈接：

HGNC: 13345

OMIM: 268200

KEGG: hsa:23175

STRING: 9606.ENSP00000256720

UniGene: Hs.467740

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