ITGA5 Recombinant Monoclonal Antibody

說明書

中文名稱：

ITGA5重組抗體
貨號(hào)：

CSB-RA011868MA1HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA011868MA1HU diluted at 1:50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

ITGA5 Recombinant Monoclonal Antibody（CSB-RA011868MA1HU）是一款特異性靶向整合素α5（ITGA5）的高性能抗體，適用于多種科研場(chǎng)景。ITGA5是整合素家族的重要成員，通過與β1亞基形成異源二聚體參與細(xì)胞-基質(zhì)粘附、遷移及信號(hào)轉(zhuǎn)導(dǎo)，在腫瘤侵襲、血管生成和組織修復(fù)等過程中發(fā)揮關(guān)鍵作用。本抗體經(jīng)ELISA和免疫組化（IHC）技術(shù)嚴(yán)格驗(yàn)證，在IHC實(shí)驗(yàn)中表現(xiàn)出優(yōu)異的組織特異性，推薦使用稀釋度為1:50-1:200，能夠清晰定位ITGA5蛋白在石蠟包埋組織切片中的表達(dá)分布。其重組單克隆特性確保了批次間的高度一致性，為研究ITGA5在癌癥微環(huán)境、纖維化疾病或胚胎發(fā)育中的功能提供了可靠工具。該產(chǎn)品可廣泛應(yīng)用于細(xì)胞生物學(xué)、病理機(jī)制探究及藥物靶點(diǎn)篩選等領(lǐng)域，例如分析腫瘤細(xì)胞遷移能力、評(píng)估血管生成活性或探索細(xì)胞外基質(zhì)互作的分子調(diào)控網(wǎng)絡(luò)，為相關(guān)基礎(chǔ)研究提供精準(zhǔn)的實(shí)驗(yàn)支持。
Uniprot No.：

P08648
基因名：

ITGA5
別名：

ITGA5 Recombinant Monoclonal Antibody
反應(yīng)種屬：

Human
免疫原：

Recombinant Human ITGA5 protein
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

hIgG1
純化方式：

Affinity-chromatography
克隆號(hào)：

1E12
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling.; (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus.; (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19.; (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
基因功能參考文獻(xiàn)：
1. The results showed that in cervical cancer cells Rac1 activation by hypoxia could stimulate invasion and migration, and this process was mediated by integrin a5b3-facilitated FAK and PI3K phosphorylation. PMID: 29358562
2. A specific component of the gastric refluxate, deoxycholic acid, affects the epithelial barrier through modulation of integrin alpha V expression, providing a novel mechanism for bile acid-mediated erosion of esophageal squamous epithelium and promotion of Barrett's Esophagus. PMID: 28941013
3. first PET/CT scans of head and neck squamous cell carcinoma (HNSCC) and NSCLC patients displayed SFITGv6 accumulation specifically in tumors, but not in inflammatory lesions.Thus, SFITGv6 represents a novel powerful tracer for imaging and possibly for endoradiotherapy of ITGa5b6-positive carcinoma PMID: 28468949
4. Endothelial cell-derived matrix promotes the metabolic functional maturation of hepatocyte via integrin-Src signaling. PMID: 28470937
5. integrin alpha5 might be involved in Aggregatibacter actinomycetemcomitans Y4 invasion into gingival epithelial cells, and the resulting signal transduction cascade reduces cell adhesion by decreasing the expression of integrins, while the TLR2/4 signaling cascade regulates IL-8 expression. PMID: 28593565
6. Fibronectin fragments (FNFr) function as matrikines driving the chemotactic affinity of prostate cancer cells via the alpha5beta1 integrin. PMID: 27715399
7. Gene function analyses in in-vitro cultured adipose tissue stem cells (ASCs) indicated differential functions of integrin-alpha-5 (ITGA5) and integrin-alpha-V (ITGAV). PMID: 27363302
8. Hypoxia induces the expression of ITGA5 in breast cancer.ITGA5 plays role in the breast cancer metastasis. PMID: 28213554
9. miR-330-5p could suppress proliferation and invasion of glioblastoma cells through targeting ITGA5. PMID: 28336765
10. Inhibiting Fgf-R partly reversed alphavbeta3 integrin activity in Mll-Ell+ progenitor cells. PMID: 27340869
11. Thus, Aspergillus fumigatus CalA is an invasin that interacts with integrin alpha5beta1 on host cells, induces endocytosis and enhances virulence. PMID: 27841851
12. Data map the network of interactions between host-cell alpha5beta1 integrin and the cagT4SS proteins. PMID: 29055076
13. Data indicate integrinalphavbeta3 is a tumor target on breast cancer bone metastases. PMID: 28855208
14. we compared the characteristics of tumors with and without wild-type (wt) cANGPTL4 and tumors with cANGPTL4 bearing the T266M mutation (T266M cANGPTL4). We found that T266M cANGPTL4 bound to integrin alpha5beta1 with a reduced affinity compared to wt, leading to weaker activation of downstream signaling molecules. PMID: 28641978
15. Osteoprotegerin facilitates pulmonary arterial hypertension pathogenesis by regulating pulmonary arterial smooth muscle cell proliferation via integrin alphavbeta3/FAK/AKT signaling pathway. PMID: 28077433
16. alpha5beta1 integrin can stimulate drug resistance in cancer cells via a mechanism based on inhibition of protein kinase Erk. PMID: 28988530
17. We measured the effect of recombinant Gal-3 added exogenously on the lateral mobility of the alpha5b1 integrin on HeLa cells. Using single-particle tracking (SPT) we detected increased lateral mobility of the integrin in the presence of Gal-3, while its truncated C-terminal domain (Gal-3C) showed only minor reductions in lateral mobility. PMID: 29016609
18. pStat-3 and integrin alphavbeta6 were indicators of tumor's progression and poor prognosis of patients with gallbladder cancer PMID: 28061445
19. Eps8/Abi1/Sos1 tricomplex acts as a key molecular switch altering the balance between Rac1 and Rho activation; its presence or absence in pancreatic ductal adenocarcinoma cells modulates alphavbeta6-dependent functions, resulting in a pro-migratory (Rac1-dependent) or a pro-TGF-beta1 activation (Rho-dependent) functional phenotype. PMID: 28608476
20. Our study is the first to report that treatment with a RARgamma specific agonist augments cellular adhesion to alpha5beta1 integrin substrates, increases cell surface levels of the beta1 integrin subunit, and dampens cellular proliferation in a time and concentration dependent manner in a human erythroleukemia cell line PMID: 28552962
21. Findings indicate integrin inhibition as a promising strategy to block both aryl hydrocarbon receptor (AhR) and transforming growth factor beta (TGF-beta)-controlled features of malignancy in glioblastoma. PMID: 26500056
22. Analysis of alphavbeta3 and alpha5beta1 integrin clusters indicates that fibrillar adhesions are more prominent in cells adhering to alpha5beta1 ligand, while clusters are mostly localized at the cell margins in cells adhering to alphavbeta3 ligand. PMID: 27003228
23. Suggest an adhesion-dependent mechanism of RUNX2 for the osteotropism and bone colonization of breast cancer cells and implicate RUNX2 and integrin alpha5 as potential molecular markers for the prediction of bone metastasis. PMID: 27317874
24. cells on fibronectin suppressed cAMP via enhanced phosphodiesterase (PDE) activity, through direct binding of integrin alpha5 to phosphodiesterase-4D5 PMID: 27595237
25. N-Glycosylation on integrin alpha5 is required for alpha5beta1-mediated cell migration. PMID: 28167607
26. Data show that high mobility group nucleosomal binding domain 2 (HMGN2) knockdown induced the increased expression of alpha5beta1 integrin on cell membranes, which resulted in a significant increase in Klebsiella pneumoniae internalization. PMID: 27460641
27. ITGA5 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
28. ectopic expression of miR-17 in ovarian cancer cells resulted in repressed ILK phosphorylation as well as decreased production of active matrix metalloproteinase-2 (MMP-2). Our results indicated that miR-17 hampered ovarian cancer peritoneal propagation by targeting integrin a5 and b1. PMID: 27499367
29. These results suggest that downregulation of miR-330-5p expression may affect Colorectal cancerdevelopment via modulation of ITGA5 expression. PMID: 27633518
30. In colorectal cancer cells, miR-130b directly targeted the 3' untranslated region (3'UTR) region of integrin alpha5 gene, which encodes a key molecule involved in cell motility. TGF-beta1 acted through miR-130b to promote integrin alpha5 expression, resulting in the enhanced migration of CRC cells. PMID: 26873488
31. Suggest that in gliomas, integrin alpha5-mediated dissemination from the tumor mass overrides integrin alpha5-mediated tumor cell cohesion. PMID: 27063097
32. upregulation of miR-31 targeting ITGA5 may suppress tumor cell invasion and metastasis by indirectly regulating PI3K/AKT signaling pathway in human SGC7901 GC cells PMID: 26729197
33. The aim of the present study was to characterize the roles of ITGalpha5 and ITGbeta1 in the proliferation and migration of vascular smooth muscle cells. PMID: 26648324
34. ITGA5 plays an important role in maintaining and regulating the normal migration capacity of dental pulp stem cells. PMID: 26823759
35. IGFBP-2 enhances endothelial progenitor cell adhesion to HUVECs via Integrin alpha5beta1. The RGD motif in the IGFBP-2 C-domain is required for interaction with integrin alpha5beta1. PMID: 26076738
36. The present study has revealed an integrin- and membrane lipid raft-dependent mechanotransduction mechanism by which atheroprone flow causes endothelial dysfunction. PMID: 26733684
37. Data show that the fold changes of very late antigen 5 VLA-5 (integrin alpha-5 beta-1) in CD14+ monocytes were higher than those observed for THP-1 cells. PMID: 26159767
38. integrin alpha5 might play important roles in the progression of esophageal squamous cell carcinoma PMID: 26772401
39. Data suggest that reduced ability of AnxA6-expressing cells to migrate is associated with decreased cell surface expression of integrins alphaVbeta3 and alpha5beta1. PMID: 26578516
40. Data suggest that the binding, but not the internalization of photoreceptor outer segments (POS) confers protective effects on retinal pigment epithelium (RPE) cells through the alphavbeta5 integrin/focal adhesion kinase (FAK)/PGC-1alpha pathway. PMID: 26244551
41. alpha5beta1 and alphavbeta3 each mediate actin cytoskeletal remodeling in response to stiffening or cyclic stretching of the extracellular matrix. PMID: 25663698
42. Integrin alpha5beta1 was expressed on ligamentum flavum fibroblasts. PMID: 25735609
43. Data suggest that Entamoeba histolytica-macrophage contact triggers recruitment of alpha5beta1 integrin (ITGA5/ITGB1) and NLRP3 into intercellular junction, where ITGA5/ITGB1 undergoes activation by cysteine protease on parasite surface. PMID: 25955828
44. Data show that thyroid hormones induce mitogen-activated protein kinase (MAPK) via integrin alphavbeta3 in bone marrow-derived cells from multiple myeloma (MM). PMID: 25058375
45. Integrin alphanubeta6-mediated MMP-9 secretion facilitates pericellular matrix degradation at high cell density, promoting invasive growth of colonic neoplasms. PMID: 26139991
46. Data show the molecular model of the complex of activated protein C (APC) with alphaVbeta3 integrin obtained by protein-protein docking approach. PMID: 24251463
47. Data indicate that alpha5 integrin (ITGA5) is a functional target of miroRNA miR-26a-induced anoikis in hepatocellular carcinoma (HCC) cells. PMID: 25537511
48. Collagens considerably suppressed alpha5 gene expression in CECs, suggesting that during wound PMID: 26431475
49. Suggest alpha5beta1/Arp2/Arp3/FHOD3 pathway reprograms the actin cytoskeleton to promote invasive migration and local invasion in vivo. PMID: 26370503
50. In the presence of both 9-cis-retinoic acid and troglitazone, cell surface alpha5 subunit expression was restored to levels comparable to vehicle treatment alone. PMID: 24671180
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亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein. Cell junction, focal adhesion. Cell surface.
蛋白家族：

Integrin alpha chain family
數(shù)據(jù)庫(kù)鏈接：

HGNC: 6141

OMIM: 135620

KEGG: hsa:3678

STRING: 9606.ENSP00000293379

UniGene: Hs.505654

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