HK1 Recombinant Monoclonal Antibody

說明書

中文名稱：

HK1 Recombinant Monoclonal Antibody
貨號：

CSB-RA825804A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: U-87MG whole cell lysate(30μg), HT-29 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg), Mouse brain tissue lysate(30μg), Rat brain tissue lysate(30μg)
All lanes: Hexokinase 1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 102 kDa
Observed band size: 120-130 kDa
Exposure time：2min

IHC image of CSB-RA825804A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing MCF-7 cells stained with CSB-RA825804A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

P19367
基因名：

HK1
別名：

Hexokinase-1 (EC 2.7.1.1) (Brain form hexokinase) (Hexokinase type I) (HK I), HK1
反應種屬：

Human, Mouse, Rat
免疫原：

A synthesized peptide from human HK1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

14E8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome.
基因功能參考文獻：
1. Mutation in c-Src phosphorylation site of either HK1 or HK2 remarkably abrogates the stimulating effects of c-Src on glycolysis, cell proliferation, migration, invasion, tumorigenesis and metastasis PMID: 28054552
2. HK1 competes with SOD1 G93A mutant from familial amyotrophic lateral sclerosis cases for binding VDAC1. PMID: 27721436
3. HK1 protein levels was decreased after luteolin treatment and partly restored when co-treated with luteolin and miR-34a inhibitor. PMID: 29054762
4. The pathogenic nature of the identified missense mutations was confirmed by biochemical and 3-dimensional structural analysis. The effects of the novel splice site mutation c.873-2A>G were studied at the level of pre-mRNA processing, and confirmed at the protein level. Four of the 6 mutations studied were new. PMID: 27282571
5. STAT3 expression is upregulated in both HBV- and HCV-related hepatocellular carcinoma (HCC), while hexokinase II (HK-II) is predominantly upregulated and correlated to STAT3 in HBV-related HCC PMID: 26889748
6. Russe type hereditary motor and sensory neuropathy belongs to the most frequent types of hereditary neuropathy in the Czech Republic, which affects Roma and is caused by a HK1 mutation. PMID: 26822750
7. Data suggest that overexpression of hexokinase 1 (HK1) may act as a significant biomarker of poor prognosis for patients with colorectal cancer (CRC). PMID: 26476538
8. 4-hydroxytamoxifen resistance in breast cancer cells is suppressed by curcumin, which targets the SLUG/Hexokinase 2 pathway PMID: 27012210
9. Our results suggest that overexpression of PKM2 and HK1, especially the latter, significantly associates with lymphatic metastasis, advanced clinical staging and unfavorable prognosis in gastric cancer. PMID: 26464675
10. The transport of glucose across the cell membrane by glucose transporters (GLUTs) and intracellular phosphorylation by hexokinases (HKs) are the initial steps of the glycolytic pathway. PMID: 25766729
11. HK1 and HK2 expression alterations were detected, that could be explained by common deregulation mechanisms of these genes in colorectal tumors. The HK3 expression level was significantly increased in 60% of samples. PMID: 26855992
12. Data indicate a missense mutation in hexokinase 1 (HK1) c.2539G > A, p.Glu847Lys, tracking with disease in all affected family members. PMID: 26427411
13. HK1 expression is highly enriched in neurons compared to astrocytes in the cerebral cortex. PMID: 25904018
14. HK-1 plays a non-metabolic role in HIV-1 infected macrophages by binding to mitochondria thereby maintaining mitochondrial integrity. PMID: 25602755
15. The present study was designed to evaluate the involvement of hexokinase and CPT-1 in the cell growth and proliferation of human prostate cancer cell lines, PC3, and LNCaP-FGC-10. PMID: 25501281
16. HK1 is expressed in retina, with two abundant isoforms expressed at similar levels. The Glu847Lys mutation is located at a highly conserved position in the protein, outside the catalytic domains. PMID: 25190649
17. Here, we identified HK1 as a novel causative gene for adRP. This is the first report that associates the glucose metabolic pathway with human retinal degenerative disease, suggesting a potential new disease mechanism. PMID: 25316723
18. Hexokinase 1 (HK1) is a mitochondrial protein that controls the tramsmembrane potential and blocks apoptotic signals at the mitochondria. PMID: 24018046
19. An increased ratio of HK1 protein in the extrasynaptic membrane/mitochondrial fraction of prefronal cortex was found in subjects with schizophrenia, suggesting that HK1 protein is abnormally partitioned in this illness PMID: 24560881
20. A genetic association of myelomeningocele in Chile found polymorphisms for HK1. PMID: 23427181
21. We detected mutation in the NDRG1 gene in two families and mutation in the HK1 gene in the other two families. These mutations cause Charcot-Marie-Tooth Disease, Type 4D and Charcot-Marie-Tooth disease, type 4g, respectively. PMID: 23996628
22. Dominant, diazoxide-responsive HI in this family maps to a novel locus on Chr10q21-22. Disease-associated variants were found in non-coding regions of HK1 PMID: 23859901
23. Data indicate that the most frequent form is SH3TC2 gene (CMT4C; 57.14%), followed by HK1 gene causative of CMT4G (CMT4G/HMSN-Russe 25%) and NDRG1 p.R148X in CMT type 4D (CMT4D/HMSN-Lom; 17.86%). PMID: 22978647
24. The screening for novel Parkin substrate(s) identified mitochondrial hexokinase I (HKI) as a candidate. PMID: 23068103
25. A key K8/K18 regulation of glucose metabolism in normal and cancerous hepatic cells through differential modulations of mitochondrial hexokinase status and insulin-mediated signaling. PMID: 23164509
26. This study proposed that HK1 mitochondrial detachment could be linked to these disorders through impaired energy metabolism, increased vulnerability to oxidative stress, and impaired brain growth and development. PMID: 22018957
27. An association is noted between the rs7072268 T-allele in HK1 and an increased blood glucose in non-diabetic individuals and a nominal association with type 2 diabetes prior to Bonferroni correction. PMID: 21781351
28. Abeta-induced cellular redistribution and inactivation of neuronal HKI play important roles in oxidative stress and neurodegeneration in Alzheimer's disease PMID: 21179577
29. FimA strengthens the VDAC1-hexokinase(I and II) interaction and prevents dissociation of hexokinase from VDAC1 triggered by apoptotic stimuli. PMID: 20347420
30. The comprehensive sequencing analysis and fine mapping approximately 26 kb of fully characterised sequence spanning the upstream exons of Hexokinase 1 (HK1), is reported. PMID: 19536174
31. REVIEW: Gene expression and biological significance of HK1 and HKR isoforms of hexokinase in erythroid cells. PMID: 12432216
32. Glucose 6-phosphate binds to human brain hexokinase in mitochondria PMID: 16166083
33. The role of the HK1 interdomain alpha-helix at the interdomain signal transduction was studied. PMID: 17080299
34. Neither human hexokinase-1 nor human inorganic pyrophosphatase expression segregated concordantly with human cytoplasmic glutamic-oxaloacetic transaminase expression. PMID: 17494625
35. single nucleotide polymorphisms on gene involved with glucose metabolism and obesity may be associated with increased susceptibility to spina bifida PMID: 18212354
36. interference with the binding of Hexokinase-I to mitochondria by VDAC1-derived peptides may offer a novel strategy by which to potentiate the efficacy of conventional chemotherapeutic agents PMID: 18308720
37. results point to HK-I and HK-II as promoting tumor cell survival through binding to VDAC1, thereby inhibiting cytochrome c release and apoptotic cell death. PMID: 19049977
38. Glycated hemoglobin levels are associated with genetic variation of HK1. PMID: 19096518
39. The expression pattern of HK1 is reported in newly diagnosed esophageal adenocarcinoma by means of immunohistochemistry. PMID: 19554504
40. reduced erythroid transcription of HK1 together with aberrant splicing of both hexokinase-1 and red cell specific-hexokinase results in hexokinase deficiency and mild chronic hemolysis. PMID: 19608687
41. HK1 may influence A1C levels through its anemic effect or its effect on glucose metabolism in erythrocytes, which may have implications for type 2 diabetes diagnosis and care. PMID: 19651813
42. Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19165527
43. This protein has been found differentially expressed in the temporal lobe from patients with schizophrenia. PMID: 19034380
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相關疾病：

Hexokinase deficiency (HK deficiency); Neuropathy, hereditary motor and sensory, Russe type (HMSNR); Retinitis pigmentosa 79 (RP79)
亞細胞定位：

Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasm, cytosol.
蛋白家族：

Hexokinase family
組織特異性：

Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific. Isoform 4: Testis-specific.
數據庫鏈接：

HGNC: 4922

OMIM: 142600

KEGG: hsa:3098

STRING: 9606.ENSP00000384774

UniGene: Hs.370365

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