HES1 Recombinant Monoclonal Antibody

說明書

中文名稱：

HES1 Recombinant Monoclonal Antibody
貨號：

CSB-RA194900A0HU
規格：

￥1320
圖片：
Immunofluorescence staining of MCF-7 cell with CSB-RA194900A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing MCF-7 cells stained with CSB-RA194900A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

Q14469
基因名：

HES1
別名：

Transcription factor HES-1 (Class B basic helix-loop-helix protein 39) (bHLHb39) (Hairy and enhancer of split 1) (Hairy homolog) (Hairy-like protein) (hHL), HES1, BHLHB39 HL HRY
反應種屬：

Human
免疫原：

A synthesized peptide from human HES1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7B5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IF, FC

推薦稀釋比：

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.
基因功能參考文獻：
1. MiR-182 alleviates the development of cyanotic congenital heart disease by suppressing HES1 PMID: 30107165
2. The results of this study demonstrate that HES1 is a specific downstream gene of NOTCH1 and that it contributes to SACC proliferation, apoptosis and metastasis. PMID: 29665790
3. HES1 is mono-ubiquitinated in a Fanconi anemia core complex-dependent manner. PMID: 29463306
4. Hes-1 knockdown promotes osteopontin expression in HUVECs and enhances OPN-induced angiogenesis. PMID: 28420872
5. Our results suggest that KN promotes goblet cell differentiation by regulating Wnt, Notch, and AhR signals and expression of Hes1 and Hath1. PMID: 29436668
6. In the present study, the authors reported the first observation of Hes1 oscillatory expression in human neural progenitor /stem cells, with an approximately 1.5 hour periodicity and a Hes1 protein half-life of about 17 (17.6 +/- 0.2) minutes. Human cytomegalovirus infection disrupts the Hes1 rhythm and down-regulates its expression. PMID: 28451898
7. A three-molecule score based on the expression of Notch pathway molecules: Jagged1, intracellular Notch1 (ICN1) and Hes1 (JIH score) to assess prognostic value in non-metastasis clear cell renal cell carcinoma (ccRCC). PMID: 27612417
8. we demonstrate for the first time an essential role of HPV oncoprotein E6 that selectively overexpresses in CaCxSLCs and participates in maintenance of stem cell phenotype and stemness through upregulation of Hes1 while preponderance of E7 leads to differentiation. PMID: 26988248
9. IE1 is involved in Hes1 degradation by assembling a ubiquitination complex and promoting Hes1 ubiquitination as a potential E3 ubiquitin ligase, followed by proteasomal degradation of Hes1. PMID: 28750047
10. Notch signaling and Id2/3 regulate neurogenesis in a complementary manner and ID factors can induce neural stem cell maintenance and quiescence in the absence of Notch. PMID: 28974640
11. The phenotype was rescued by ectopic expression of miRNA182-5p in Delta182 cells. A bioinformatic analysis and Hes1 modulation data suggested that Hes1 could be a putative target of miRNA182-5p. A reciprocal relationship between miRNA182-5p and Hes1 was seen in the context of TK inhibition PMID: 28079885
12. These results suggest that HES1 promotes extracellular matrix protein expression and inhibits proliferative and migratory functions in the trabecular meshwork cells under oxidative stress, thereby providing a novel pathogenic mechanism underlying and a potential therapeutic target to primary open-angle glaucoma PMID: 28423527
13. Hes1 disruption leads to tumor regression without perturbing normal stem cell homeostasis, preclinically validating Hes1 as a cancer therapeutic target. PMID: 28536281
14. Low Hes1 expression is associated with left ventricle hypertrabeculation/non-compaction and Menetrier-like gastropathy. PMID: 28013292
15. GPR146 has an antiviral role in fighting against viral infection, although the GPR146-mediated protection is eliminated by IRF3/HES1-signalling. PMID: 28464285
16. In SNSCC, the subgroup of patients with high expression (5th quintile) of HES1 mRNA was associated with better survival (P = .04); however these patients with high expression of HES1 mRNA had also a more favorable tumor stage and grade and more unfavorable resections representing potential confounders. PMID: 27567696
17. Loss of nuclear expression of Hes1 occurs in 83% of colorectal adenocarcinomas and is associated with female sex, right-sided location, BRAFV600E mutation, microsatellite instability, larger tumor size, and significantly worse survival. PMID: 27476040
18. Hes1 plays a key role in acinar cell integrity and plasticity on cellular insults. PMID: 27639167
19. miR-182 reveals its oncogenic capacity in medullary thyroid carcinoma by directly contributing to the invasive behavior through loss of the tumor suppressive HES1/Notch1 signaling circuit. PMID: 28122586
20. Cells could therefore regulate the proportion of Wnt- and Notch-mediated control of the Hes1 promoter to coordinate the timing of cell fate selection as they migrate through the intestinal epithelium and are subject to reduced Wnt stimuli. Furthermore, mutant cells characterised by hyperstimulation of the Wnt pathway may, through coupling with Notch, invert cell fate in neighbouring healthy cells, enabling an aberrant cel PMID: 28245235
21. We demonstrated that ZNF70 interacts with ZFP64 and activates HES1 transcription by binding to the HES1 promoter. In addition, HES1 gene expression is increased in ILDR2-knockdown HepG2 cells, in which ZNF70 is translocated from the cytoplasm to the nucleus, suggesting that ZNF70 migration to the nucleus after dissociating from the ILDR2-ZNF70 complex activates HES1 transcription. These results support a novel link betwee PMID: 27353377
22. Results suggest that Hes1 plays an important role in the invasion and metastasis of NPC through inhibiting PTEN expression to trigger EMT-like phenotypes. PMID: 26452025
23. High expression of Hes1 is associated with radiation resistance in pancreatic cancer. PMID: 27677287
24. Low HES1 expression is associated with acute myeloid leukemia. PMID: 26678889
25. Results provide functional and mechanistic links between Hes1 and Bmi-1/PTEN/Akt/GSK3beta signaling in the development and progression of colon cancer. PMID: 26452029
26. HES1 DMROI methylation predicted differences in early infant behaviour, known to be associated with academic success. In vitro, methylation of HES1 inhibited ETS transcription factor binding, suggesting a functional role of this site. PMID: 25906782
27. High Hes1 expression is associated with chronic myeloid leukemia. PMID: 25849484
28. Suggest that loss of Hes1 could be used as a sensitive and specific marker to differentiate sessile serrated adenomas/polyps from hyperplastic polyps. PMID: 26448192
29. OR1A1 activation suppresses hepatic triglyceride metabolism by modulating HES-1, PPARG, and mtGPAT expression. PMID: 25817041
30. Decreased HES1 expression is associated with higher lung metastasis in hepatocellular carcinoma PMID: 25987042
31. Pancreatic stellate cells promoted Hes 1 expression in both PANC-1 and BxPC-3 cell lines and induced chemoresistance to gemcitabine. High Hes 1 expression was seen in pancreatic cancer patients with shorter overall and progression-free survival times. PMID: 25672829
32. Interference with Notch1 increased the expression level of Hes-1. PMID: 25920606
33. The results from this study indicate that Hes1 plays a quantitative role in the development and progression of colon cancer and the maintenance of the stemness of cancer stem cells, which remains to be fully characterized. PMID: 24492635
34. we summarize the recent data supporting the idea that Hes1 may have an important function in the maintenance of cancer stem cells self-renewal, cancer metastasis, and epithelial-mesenchymal transition (EMT) process induction PMID: 25781910
35. overexpression of HES1 in primary AML cells inhibited growth of AML in a xenograft mice model. In conclusion, we demonstrated the tumor suppressor role of HES1 in AML. PMID: 26092281
36. This study is the first report that elucidated the HES1 underexpression in ESCC and revealed its correlation with the invasiveness of ESCC. PMID: 25139105
37. Hairy and Enhancer of Split 1 (HES1), a transcriptional repressor controlled by NOTCH1, is a critical mediator of NOTCH1-induced leukemogenesis strictly required for tumor cell survival. PMID: 25784680
38. CPEB1 specifically suppressed the translation of HES1 and SIRT1 by interacting with a cytoplasmic polyadenylation element. PMID: 25216517
39. Hes1 cooperated with CaMK2delta to modulate OA pathogenesis through induction of catabolic factors, including Adamts5, Mmp13, Il6, and Il1rl1 PMID: 25733872
40. Hes-1 protein expression was gradually increased from normal to dysplasia to oral carcinoma, revealing Hes-1 role in the progression of oral cancer. PMID: 25037575
41. Hes1 suppresses acute myeloid leukemia development through FLT3 repression. PMID: 25234168
42. HES1 has a role in metastasis and predicts poor survival in patients with colorectal cancer PMID: 25636905
43. Hes1 oscillation sustains a dynamic proliferation state that is well adapted for versatile fate decisions in both single- and two-cell neural progenitor systems. PMID: 25605780
44. This study revealed that Notch signaling-related molecules (including Notch1, Hes1, and others) are expressed in L929 and MRC-5 cells and that Notch signaling regulates the expression of col1alpha1 and col1alpha2 in both cell lines. PMID: 25107895
45. The tumor suppressor function of LSAMP is most likely exerted by reducing the proliferation rate of the tumor cells, possibly by indirectly upregulating one or more of the genes HES1, CTAG2 or KLF10. PMID: 24885297
46. In prostate tumors, the overexpression of PTOV1 was associated with decreased expression of HEY1 and HES1, and this correlation was significant in metastatic lesions. PMID: 24684754
47. the present results revealed, for the first time, that Hes-1 could be SUMO-modified by PIAS1 and GADD45alpha is a novel target of Hes-1 PMID: 24894488
48. Data indicate that chronic myelogenous leukemia blast crisis showed that MMP-9 was highly expressed in three, with two exhibiting high levels of HES1 expression. PMID: 24825862
49. Notch signaling can directly downregulate MUC5AC promoter activity through Hes1-dependent mechanisms. PMID: 23860410
50. Hes1 upregulation contributes to the development of FIP1L1-PDGRA-positive leukemia in blast crisis. PMID: 24486648
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亞細胞定位：

Nucleus.
數據庫鏈接：

HGNC: 5192

OMIM: 139605

KEGG: hsa:3280

STRING: 9606.ENSP00000232424

UniGene: Hs.250666

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