GSN Recombinant Monoclonal Antibody

說明書

中文名稱：

GSN重組抗體
貨號：

CSB-RA009965MA1HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: U251 whole cell lysate, MCF7 whole cell lysate
All lanes: GSN antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa

Immunofluorescence staining of hela cell with CSB-RA009965MA1HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of NIH/3T3 cell with CSB-RA009965MA1HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA009965MA1HU GSN重組單克隆抗體是針對凝溶膠蛋白（Gelsolin）研發的高特異性科研試劑，該靶點作為肌動蛋白結合蛋白家族成員，在細胞骨架重塑、細胞遷移及凋亡調控等過程中發揮關鍵作用。本產品經多種實驗平臺驗證，適用于ELISA、蛋白質印跡（WB）及免疫熒光（IF）檢測：在WB實驗中可識別天然構象的GSN蛋白，推薦使用1:1000-1:5000稀釋度，能呈現清晰的特異性檢測結果；IF實驗推薦1:20-1:200稀釋度，可精準定位目標蛋白的亞細胞分布。其高親和力特性使其成為研究細胞運動機制、細胞骨架動態變化以及相關病理模型的理想工具，尤其適用于腫瘤轉移、神經退行性疾病等領域的分子機制探索。本產品經嚴格的交叉反應性測試及批次穩定性驗證，確保實驗數據的可重復性。注意：該抗體僅限于體外科研使用，不涉及任何臨床相關應用。
Uniprot No.：

P06396
基因名：

GSN
別名：

Actin depolymerizing factor antibody; Actin-depolymerizing factor antibody; ADF antibody; AGEL antibody; Brevin antibody; DKFZp313L0718 antibody; GELS_HUMAN antibody; Gelsolin antibody; Gsn antibody
反應種屬：

Human,mouse
免疫原：

Recombinant Human GSN protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

6H4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
基因功能參考文獻：
1. the gelsolin N184K variant is subjected to the same aberrant proteolytic events responsible for the formation of amyloidogenic fragments in the previously characterized mutants. PMID: 27633054
2. Induction of cell stress alters the actin cytoskeleton in intestinal epithelial cells via changes in the actin-binding proteins villin-1 and gelsolin. PMID: 29274870
3. Gelsolin upregulation promotes radioresistance in non-small cell lung cancer cells, at least partially, through activation of phosphoinositide 3-kinase/Akt signaling. PMID: 27121073
4. Results identify GSN as target of miR-200a which inhibits the release of microvesicles in hepatocellular carcinoma cells by functionally regulating GSN. PMID: 28440466
5. Gelsolin enhances the invasive capacity of colon cancer cells via elevating intracellular superoxide (O2.-) levels by interacting with Cu/ZnSOD, and gelsolin gene expression positively correlates with urokinase plasminogen activator (uPA), an important matrix-degrading protease invovled in cancer invasion. PMID: 27391159
6. these data profile the importance of the physical interaction between HPV16 E7 and gelsolin in the acquisition of the metastatic phenotype by cervical cancer cells PMID: 27072581
7. Our study highlights gelsolin as an important pro-disseminative factor contributing to the aggressive phenotype of diffuse gastric cancer PMID: 27058427
8. study strongly supported the contribution of the genes ITGA2B, GSN and RHOA and the two pathways "regulation of actin cytoskeleton" and "leukocyte transendothelial migration" to osteoporosis risk. PMID: 27153759
9. SSH1 binds to gelsolin via actin filaments in the cytosolic fraction. Gelsolin promoted solubilization of actin filaments and SSH1 in cell-free assays and in cultured cells. PMID: 25451266
10. free actin likely contributes to impaired host defense by blocking scavenger receptor binding of bacteria. PMID: 28385809
11. analysis of how YopO disrupts normal gelsolin function to alter host actin dynamics and thus cripple phagocytosis PMID: 28280241
12. Our present study implied that the synergistic effect of pGSN and pIgA induced glomerular fibrosis via the TGF-beta1/Smads signal transduction pathway. This might be a potential mechanism for the glomerular fibrosis observed in IgAN patients. PMID: 28208683
13. The expression levels of GSN were observed to be reduced in colon carcinoma (CC) cells, and the reduced expression level of GSN was often associated with a poorer metastasisfree survival rate in patients with CC (P=0.04). In addition, the overexpression of GSN inhibited the invasion of CC cells in vitro. PMID: 27573444
14. in chronic hemodialysis patients, lower pGSN levels were not associated with hospitalization, all-cause and cardio-vascular mortality, even though pGSN was inversely correlated with age, CRP and IL-6, suggesting that inflammation may influence pGSN PMID: 28114138
15. PODXL enhances motility and invasiveness through an increase in gelsolin-actin interactions in cell protrusions. PMID: 27461278
16. These findings suggest that gelsolin may have a role in the disease process in atopic dermatitis patients. PMID: 26318415
17. Plasma GSN may play an important role in the development of immunoglobulin A (IgA) nephropathy. PMID: 27997897
18. beta-Catenin seems to be involved in the regulation of gelsolin expression, which in turn affects the migratory ability of colonic cancer cells PMID: 27798885
19. hypoxia or GSN overexpression induces HIF-1alpha expression and reduces the expression of survival markers p-Akt and Bcl-2 in H9c2 cardiomyoblast cells PMID: 27193608
20. Increasing amount of amyloid are associated with the severity of clinical features in hereditary gelsolin amyloidosis. PMID: 27879149
21. The rs1078305 and rs10818524 SNPs of GSN were associated with increased risk for oral squamous cell carcinoma development in a Chinese Han population PMID: 26848502
22. Lower blood levels of gelsolin associated with progressive aortic arch calcification. PMID: 26941566
23. Gelsolin protein, human, yielded a receiver operating characteristics value of 89% for node-positive OSCC PMID: 25223295
24. GSN overexpression suppresses apoptosis while down-regulated GSN promotes apoptosis in hepatocellular carcinoma. PMID: 26823700
25. Fragmented gelsolins may be associated with the pathogenesis of fibrosis in RA-ILD. PMID: 26666486
26. gelsolin interacts with particular components of the three cytoskeleton systems and is a constituent of midbodies PMID: 26598132
27. novel roles for actin-depolymerizing factor and cofilin-1 in regulating the remodeling and permeability of epithelial junctions PMID: 26878213
28. The overexpression of GSN may inhibit the proliferation, adhesion ability and invasion of 786-0 clear cell renal cell carcinoma cell line. PMID: 26398833
29. Serum-expressed apolipoprotein B-100 protein, C9 Complement, and gelsolin can be used for differential diagnosis of Barrertts esophagus and adenocarcinoma of esophagus. PMID: 26404905
30. TGF-beta1 induced epigenetic modification of GSN could alter the EMT process in breast cancer cells. PMID: 26482896
31. GSNs might play a regulatory role in the suppression of the tissue damage induced by acute radiation exposure PMID: 25164111
32. We designed and synthesized siRNA against various exons in the gelsolin gene (GSN)..our finds reveal siRNA, which is derived from target gene exon, can form the complex with H3 histone to be involved in the regulation of gene expression PMID: 25600697
33. Results show that high gelsolin levels are associated with better prognosis in ER+HER2- breast cancer and a reduction in tumor cell migration. PMID: 26408687
34. Gelsolin expression promoted tumor-associated phenotypes by facilitating proliferative and invasive capacities of hepatocellular carcinoma(HCC) cells, which might serve as a potential therapeutic target for HCC treatment. PMID: 26149653
35. Based on the obtained results, we propose that the gelsolin is an important cellular target for cotinine, through which this compound influences on the basic processes involved in neoplastic transformation and metastasis, such as migration and apoptosis PMID: 25544037
36. Group B Streptococcus-beta-haemolysin is solely responsible for gelsolin increase causing, through membrane permeability defects, calcium influx and calpain activation. PMID: 25130983
37. Plasma gelsolin levels decrease in the blood of type II diabetics. Recombinant gelsolin helped in improving glycemic control in diabetic mice. PMID: 25478578
38. Data show that gelsolin (GSN) plays an important role in the regulation of gynecological cell fate as reflected in dysregulation in chemosensitivity. PMID: 25246592
39. Data indicate gelsolin in contributing to the invasive potential of LNCaP prostate adenocarcinoma cells. PMID: 25581609
40. Gelsolin levels were a useful tool to predict functional outcome and mortality after aneurysmal subarachnoid hemorrhage. PMID: 23880145
41. increased plasma levels after allergen-specific immunotherapy PMID: 24980225
42. Patients developing cardiopulmonary bypass acute lung injury had lower plasma gelsolin reservoir and a much more amount and rapid consumption of plasma gelsolin early after operation PMID: 25126004
43. This study improves the knowledge of the genetic features of Mexican patients with corneal stromal dystrophies by identifying mutations in the TGFBI, CHST6, and GSN genes. PMID: 24801599
44. GSN is important for chemoresistance in head-and-neck cancer PMID: 24771612
45. we predict that the gene silencing of GSN and/or the downstream blocking of GSN along the p38 pathway could be applied to ameliorate pathological cardiac hypertrophy in the future PMID: 24505034
46. Ceruloplasmin and gelsolin are closely interacted with the oncogene NF-kappab. PMID: 23925487
47. Plasma gelsolin is lower in normal pregnancy than in non-pregnant women. It is also significantly lower in pre-eclampsia than in normal pregnancy. PMID: 24239294
48. This study found significantly lower plasma gelsolin levels in patients with systemic lupus erythematosus and rheumatoid arthritis compared with healthy controls PMID: 24122723
49. Expression of mutant form of human gelsolin in mice under the control of a muscle-specific promoter induced myopathic changes reminiscent of human inclusion body myositis. PMID: 24047347
50. Gelsolin can constitute a barrier that restricts HIV-1 infection of CD4+ lymphocytes in a pre-fusion step. PMID: 23575248
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相關疾病：

Amyloidosis 5 (AMYL5)
亞細胞定位：

[Isoform 2]: Cytoplasm, cytoskeleton.; [Isoform 1]: Secreted.
蛋白家族：

Villin/gelsolin family
組織特異性：

Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
數據庫鏈接：

HGNC: 4620

OMIM: 105120

KEGG: hsa:2934

STRING: 9606.ENSP00000362924

UniGene: Hs.522373

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