GATA1 Recombinant Monoclonal Antibody

說明書

中文名稱：

GATA1 Recombinant Monoclonal Antibody
貨號：

CSB-RA572727A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: K562 whole cell lysate(20μg),A549 whole cell lysate(20μg), PC-3 whole cell lysate(20μg),U-251MG whole cell lysate(20μg),SH-SY5Y whole cell lysate(20μg)
All lanes: GATA1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 43 kDa
Observed band size: 55 kDa
Exposure time：2min

Immunofluorescence staining of K-562 cell with CSB-RA572727A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing K562 cells stained with CSB-RA572727A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

P15976
基因名：

GATA1
別名：

Anemia; X-linked; without thrombocytopenia; included antibody; ERYF 1 antibody; Eryf1 antibody; Erythroid transcription factor antibody; Erythrold transcription factor 1 antibody; GATA 1 antibody; GATA binding factor 1 antibody; GATA binding protein 1 (globin transcription factor 1) antibody; GATA binding protein 1 antibody; GATA-1 antibody; GATA-binding factor 1 antibody; GATA1 antibody; GATA1_HUMAN antibody; GF 1 antibody; GF-1 antibody; GF1 antibody; Globin transcription factor 1 antibody; NF E1 antibody; NF E1 DNA binding protein antibody; NF-E1 DNA-binding protein antibody; NFE 1 antibody; NFE1 antibody; Nuclear factor erythroid 1 antibody; Transcription factor GATA1 antibody; XLANP antibody; XLTDA antibody; XLTT antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human GATA1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

6B11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.
基因功能參考文獻：
1. Reduced GATA-1 could be responsible for the upregulation of IRF-3 in lung adenocarcinoma cells through binding with a specific domain of IRF-3 promoter. PMID: 28566697
2. described the functional interaction between GATA1 and SEC23B genes in two patients with suspected congenital dyserythropoietic anemia type II PMID: 28550189
3. Here, using zebrafish, murine, and human models, the authors show that erythropoietin (EPO) signaling, together with the GATA1 transcriptional target, AKAP10, regulates heme biosynthesis during erythropoiesis at the outer mitochondrial membrane. PMID: 28553927
4. expression of GATA1 effectively rescued maturation of Primary myelofibrosis megakaryocytes. PMID: 28240607
5. GATA1 is an essential downstream target of SENP1 and that the differential expression and response of GATA1 and Bcl-xL are a key mechanism underlying chronic mountain sickness pathology. PMID: 27821551
6. we herein show a long-distance regulatory region with GATA1 binding sites as being a strong enhancer for NBEAL2 expression. PMID: 28082341
7. Single-nucleotide polymorphism in GATA1 gene is associated with non-Down syndrome transient proliferative megakaryoblastic disease. PMID: 27667142
8. These findings indicate that erythroid specific activator GATA-1 acts at CTCF sites around the beta-globin locus to establish tissue-specific chromatin organization. PMID: 28161276
9. Results show that GATA1 recognizes a single GATA motif or a composite of adjacent GATA motifs and exerts its diversified bindings. These binding configurations serve as one of the critical determinants of specific transcriptional regulation. PMID: 27215385
10. Acquired and inherited GATA1 mutations contribute to Diamond-Blackfan anemia, acute megakaryoblastic leukemia, transient myeloproliferative disorder, and a group of related congenital dyserythropoietic anemias with thrombocytopenia. PMID: 28179280
11. Our results suggest that GATAl and miR-363 were involved in the regulation of hematopoiesis via the HIF-1alpha pathway in K562 cells under hypoxic condition. PMID: 27485543
12. analysis of GATA1 mutations in a cohort of Malaysian children with Down syndrome-associated myeloid disorder reveals distinctive genomic events PMID: 27353457
13. trisomy 21 perturbed hematopoietic development through the enhanced production of early hematopoietic progenitors and the upregulation of mutated GATA1, resulting in the accelerated production of aberrantly differentiated cells. PMID: 27134169
14. Data show that pyruvate kinase (PK) activity was decreased in the GATA1 hemizygous state and PKLR c.1284delA variant. PMID: 27342114
15. GATA1 mutations were identified in consecutive Down syndrome patients with transient myeloproliferative disorder or acute leukemia. PMID: 26234152
16. expression of GATA1 and SET7 was upregulated and positively correlated with VEGF expression and microvessel number in 80 breast cancer patients. GATA1 and SET7 are independent poor prognostic factors in breast cancer. PMID: 26848522
17. Molecular cytogenetic analysis of leukemic blast cells indicated that increased blast cell status was caused by transient abnormal myelopoiesis with trisomy 21 and GATA1 mutation. PMID: 25711269
18. deletion of P-sel disrupted megakaryocyte/neutrophil interactions in spleen, reduced TGF-beta content, and corrected the hematopoietic stem cells distribution that in Gata1(low) mice, as in primary myelofibrosis patients, is abnormally expanded in spleen. PMID: 26439305
19. study provides insight into GATA1 transcriptional activity and may prove a useful resource for investigating the pathogenicity of noncoding variants in human erythroid disorders. PMID: 27044088
20. The GATA-1-mediated inhibition of PU.1 gene transcription in human AML-erythroleukemias mediated through the URE represents important mechanism that contributes to PU.1 downregulation and leukemogenesis that is sensitive to DNA demethylation therapy PMID: 27010793
21. findings provide insights into the clinically relevant in vivo function of the N-terminal domain of GATA1 in human hematopoiesis. PMID: 26713410
22. Acute megakaryoblastic leukemia is associated with GATA-1 mutation, mimicking myeloproliferative disorders. PMID: 26205501
23. GATA2-to-GATA1 switch is prevalent at dynamic enhancers and drives erythroid enhancer commissioning PMID: 26766440
24. GATA1 and GATA2 are involved in clear cell renal cell carcinoma biology possibly affecting tumor development and aggressiveness. PMID: 25230694
25. Congenital erythropoietic porphyria linked to GATA1-R216W mutation PMID: 25251786
26. we uncovered a novel function of GATA1 in regulating Epithelial-mesenchymal transition PMID: 25726523
27. Global transcriptome and chromatin occupancy analysis reveal the short isoform of GATA1 is deficient for erythroid specification and gene expression. PMID: 25682601
28. EDAG forms complex with GATA1 and p300 and increases GATA1 acetylation and transcriptional activity by facilitating the interaction between GATA1 and p300 PMID: 24740910
29. These results indicate that KLF1 plays a role in facilitating and/or stabilizing GATA-1 and TAL1 occupancy in the erythroid genes, contributing to the generation of active chromatin structure such as histone acetylation and chromatin looping PMID: 25528728
30. Our case of transient leukemia without Down syndrome highlight the important role of trisomy 21 and GATA1 mutation in the development of transient neonatal leukemia. PMID: 24253371
31. In erythroid cells, pull down experiments identified the presence of a novel complex formed by HDAC5, GATA1, EKLF and pERK which was instead undetectable in cells of the megakaryocytic lineage. PMID: 24594363
32. Results demonstrate that expression of the hGATA1 gene is regulated through the chromatin architecture organized by 5'CTCF site-mediated intrachromosomal interactions in the hGATA1 locus. PMID: 25755285
33. The results demonstrate that hGATA-1 and hGATA-2 expression in hippocampus is sufficient to cause depressive like behaviors. PMID: 25340772
34. Lineage-specific GATA1 cofactor associations are essential for normal chromatin occupancy. PMID: 25621499
35. Nkx2-5 binds to the Gata1 gene enhancer and represses the transcriptional activity of the Gata1 gene. PMID: 21464046
36. a hypothesis is presented to explain that, in Down syndrome, the first mutational events, GATA1 somatic mutations, do not occur at random, but as a result of perturbed cell functions and specific over-expression of the GATA1 gene PMID: 24880866
37. Data indicate that GATA1 transcription factor is downregulated in ribosomal protein S19 (RPS19)-deficient cells through upregulation of TNF-alpha and p38 MAPK. PMID: 25270909
38. A functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p in erythropoiesis. PMID: 24608802
39. Somatic GATA1 mutations appear to be pivotal in development of transient abnormal myelopoiesis and are proving to be markers of clonal identity in its evolution to acute megakaryoblastic leukemia in subjects with Down syndrome. [CASE STUDY; REVIEW] PMID: 25268193
40. The high rate of GATA-1 gene mutations was confirmed in newborn infants with Down's Syndrome and transient abnormal myelopoiesis or acute megakaryoblastic leukemia PMID: 24196768
41. Results report a forth family with clinical findings consistent with an association between GATA1 gene mutation and anemia black diamond. PMID: 24766296
42. amplitude of a transcriptional signature of GATA1 target genes was globally and specifically reduced, indicating that the activity, but not the mRNA level, of GATA1 is decreased in patients with DBA PMID: 24952648
43. PSTPIP2 dysregulation contributes to aberrant terminal differentiation in GATA-1-deficient megakaryocytes by activating LYN. PMID: 24407241
44. High GATA1 expression is associated with hyperproliferation of eosinophil precursors in Down syndrome transient leukemia. PMID: 24336126
45. Mutations in the GATA1 gene associated with leukemogenesis in newborns with Down syndrome. PMID: 24222239
46. Loss of GATA-1 full length as a cause of Diamond-Blackfan anemia phenotype. PMID: 24453067
47. Mitochondrial translation is dramatically affected after mGatA depletion, revealing an essential role for the GatCAB enzyme in the process of protein biosynthesis in mammalian mitochondria. PMID: 24579914
48. Our results suggest that GATA1 exon 2 mutations occur late in trisomy 21 fetal hematopoiesis PMID: 24746204
49. A role for GATA1 in chemotherapy resistance in non-Down syndrome acute megakaryocytic leukemia cells. PMID: 23874683
50. multiple modes of the GATA1-MED1 axis may help to fine-tune GATA1 function during GATA1-mediated homeostasis events. PMID: 24245781
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相關疾病：

X-linked dyserythropoietic anemia and thrombocytopenia (XDAT); Thrombocytopenia with beta-thalassemia, X-linked (XLTT); Anemia without thrombocytopenia, X-linked (XLAWT)
亞細胞定位：

Nucleus.
組織特異性：

Erythrocytes.
數據庫鏈接：

HGNC: 4170

OMIM: 300367

KEGG: hsa:2623

STRING: 9606.ENSP00000365858

UniGene: Hs.765

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