FSCN1 Recombinant Monoclonal Antibody

說明書

中文名稱：

FSCN1重組抗體
貨號：

CSB-RA619084MA1HU
規格：

￥1320
圖片：
IHC image of CSB-RA619084MA1HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA619084MA1HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA619084MA1HU是一款針對FSCN1蛋白的重組單克隆抗體，適用于ELISA和免疫組化（IHC）檢測。FSCN1（Fascin Actin-Bundling Protein 1）作為細胞骨架調節蛋白，通過結合肌動蛋白微絲參與細胞遷移、粘附及形態維持，其異常表達與腫瘤侵襲轉移密切相關，在乳腺癌、結直腸癌等多種惡性腫瘤中呈現高表達特征。本抗體經特異性驗證，可在IHC實驗中清晰顯示石蠟包埋組織樣本中FSCN1的定位分布，推薦使用稀釋比例為1:20至1:200，實驗結果顯示其具備優異的批間一致性和低交叉反應性，能夠準確識別天然構象下的靶蛋白。該產品可為研究FSCN1在腫瘤微環境中的功能機制、細胞運動相關信號通路調控，以及癌癥病理模型的建立提供可靠工具，適用于基礎科研領域中蛋白表達分析、疾病標志物探索及藥物靶點相關研究。
Uniprot No.：

Q16658
基因名：

FSCN1
別名：

55 kDa actin bundling protein antibody; 55 kDa actin-bundling protein antibody; Actin bundling protein antibody; actin bundling protein; 55-KD antibody; FAN 1 antibody; FAN1 antibody; Fascin 1 antibody; Fascin actin bundling protein 1 antibody; Fascin antibody; Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) antibody; Fascin homolog 1 antibody; Fascin; sea urchin; homolog of; 1 antibody; Fascin1 antibody; FLJ38511 antibody; FSCN 1 antibody; FSCN1 antibody; FSCN1_HUMAN antibody; HSN antibody; p55 antibody; Singed (Drosophila) like (sea urchin fascin homolog like) antibody; Singed drosophila homolog like antibody; Singed like (fascin homolog sea urchin) (Drosophila) antibody; Singed like (fascin homolog sea urchin) antibody; Singed like protein antibody; Singed; drosophila; homolog of antibody; Singed-like protein antibody; SNL antibody; Strongylocentrotus purpuratus antibody
反應種屬：

Human
免疫原：

Recombinant Human FSCN1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Mouse IgG
純化方式：

Affinity-chromatography
克隆號：

7C4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF.
基因功能參考文獻：
1. fascin1 constitutively interacts with IkappaB kinase (IKK) in the RIG-I signaling pathway. In summary, we have identified fascin1 as a suppressor of the RIG-I signaling pathway associating with IkappaB kinase in DLD-1 colon cancer cells to suppress immune responses to viral infection. PMID: 29496994
2. fascin protein accumulation, caused by reduced proteasomal activity, contributes to the acquisition of cancer stemness in chronic inflammation-related colon carcinogenesis. PMID: 30056535
3. Fascin1 is related with clinicopathologic parameters of gastric cancer and overexpressed both in gastric cell lines and gastric tumor tissue PMID: 30060981
4. the expression of Fascin_1 protein differed between cancer tissue and paracarcinoma tissues in NSCLC patients and it was also correlated with poor prognosis. PMID: 29970665
5. FSCN1 physiologically interacted with and increased the levels of snail1 to promote ovarian cancer cell epithelial-mesenchymal transition . PMID: 30231243
6. fascin1 is an important mediator of TGF-beta1-induced invasion and migration of kidney carcinoma cells through ERK and JNK signal pathways. PMID: 29772238
7. findings demonstrate that fascin is required for migration and invasion induced by LA in MDA-MB-231 breast cancer cells. PMID: 29052029
8. PCAT-1 accelerated prostate cancer cell proliferation, migration, invasion and suppressed apoptosis by up-regulating FSCN1 mediated via miR-145-5p. PMID: 28922730
9. Lentivirus-mediated fascin-1 knockdown significantly decreased the proliferation of non-small cell lung cancer cells. Furthermore, fascin-1 silencing partly inhibited cell invasion and migration. Inhibition of fascin-1 decreased the activity of the MAPK pathway. PMID: 29458026
10. Study shows that Fascin 1 has a nuclear function in the regulation of the amino-acid transporter SLC3A2. PMID: 27819326
11. SNAI2 overexpression significantly increased FSCN1 expression at both mRNA and protein level. FSCN1 overexpression reduced the expression of E-cadherin and Claudin 1, but increased the expression of Vimentin and N-cadherin in SCC9 and SCC-15 cells. It is inferred that FSCN1 is a downstream effector of SNAI2 in promoting EMT in HNSC cells. PMID: 28488774
12. MYC-nick, fascin, and Cdc42 are frequently up-regulated in cells present at the invasive front of human colorectal tumors, suggesting a coordinated role for these proteins in tumor migration. PMID: 27566402
13. The prognostic importance of TGF-beta, TGF-beta receptor, and fascin in childhood solid tumors PMID: 29065267
14. The results reveal that phosphorylation at FSCN1 amino acids Y23, S38, S39 and S274, in combination, downregulates the extent of anchorage-independent growth, cell migration and filopodia formation in esophageal squamous cancer cells. PMID: 28251354
15. Fascin and alpha-actinin intrinsically segregate to discrete bundled domains that are specifically recognized by other actin-binding proteins. PMID: 27666967
16. This study showed that fascin expression intensity may be correlated with clinical outcomes of a newly diagnosed primary glioblastoma. PMID: 27406586
17. These data demonstrated fascin as a critical regulator of breast cancer stem cell pool at least partially via activation of the Notch self-renewal signaling pathway. PMID: 27502039
18. These results suggest that strong positive fascin-1 expression can be used as a diagnostic marker of TNBC. PMID: 27184764
19. Authors demonstrated that one miRNA, miR-29a-5p, can bind to both the XIAP and FSCN1 3'UTRs and play an important role in that interactions. We showed that the 3'UTR of XIAP was able to antagonize miR-29a-5p, and resulted in the increased translation of XIAP and FSCN1. PMID: 28186968
20. The expression of fascin-1 and lanminin-5 and the serum levels of fascin-1 and laminin-5 were related to the relapse of patients with non-small cell lung cancer. PMID: 28653739
21. The results uncover a role for fascin that operates independently of filopodia assembly to promote efficient cell migration and invasion. PMID: 27554857
22. FSCN1 is a direct target of miR-539.MiR-539 inhibits FSCN1 expression and suppresses hepatocellular carcinoma migration and invasion. PMID: 28393215
23. we show for the first time that HTLV-1 usurps the host cell factor Fascin to foster virus release and cell-to-cell transmission. PMID: 27776189
24. Results show that BMI1 and FSCN1 are highly expressed in colorectal neoplasm and correlate with poor prognosis. PMID: 27488323
25. miR-200b serves a suppressive role in the mediation of NSCLC cell migration and invasion, partly at least, via targeting FSCN1. PMID: 27356635
26. LRIG1 and Fascin-1 were differently expressed in cancer and normal lung tissue in patients with NSCLC, which could be a biomarker for mediastinal lymph node metastasis in NSCLC patients. PMID: 28230028
27. The increase in fascin1 expression may be the key point of this impact of TGF-beta1. PMID: 27350089
28. Fascin 1 plays an oncogenic role in non-small cell lung cancer PMID: 26886283
29. fascin expression promotes cholangiocarcinoma RBE cell proliferation, migration, and invasion. PMID: 25882880
30. TGF-beta1 could induce the expressions of Fascin1 mRNA and protein and thus improve the invasive and metastatic ability of human renal carcinoma cells. PMID: 26749573
31. elevated L-plastin expression promotes elongation and reduces protrusion density in cells with relatively lower L-plastin than fascin levels. PMID: 26945069
32. Study demonstrates that FSCN1 is associated with increased risk of recurrence in pituitary adenomas and invasion, partially through modifying the expression of Notch/DLL pathway. PMID: 26522130
33. The present study revealed an antioncogenic role of miR145 in gastric carcinoma via inhibition of FSCN1, and suggested that miR145 may be used for the treatment of gastric carcinoma. PMID: 26647829
34. miR-145 negatively regulates the protein expression of its target FSCN1 in the NSCLC cell line H129. PMID: 26238532
35. MiR-24 functions as a tumor suppressor in nasopharyngeal carcinoma through targeting FSCN1 PMID: 26503504
36. Strong fascin-1 expression was found in 14.3% of borderline epithelial tumors of the ovary and linked to features related to elevated risk of recurrence. PMID: 26362828
37. FSCN1 plays an important role in non-small cell lung cancer progression and prognosis. PMID: 26211585
38. Alterations in nuclear Fascin levels result in defects in nucleolar morphology in both Drosophila follicles and cultured mammalian cells, suggesting that nuclear Fascin plays an important role in nucleolar architecture. PMID: 25808493
39. miR-145 regulates cell migration and invasion in gastric cancer primarily by directly targeting FSCN1. PMID: 26010149
40. regulates translation through miR-155 and miR-125b, which target 3' UTR in TNF-alpha mRNA PMID: 25831081
41. the anti-oncogenic activity of miR-133a may involve the inhibition of the target gene FSCN1. The present study suggested that miR-133a may be a potential therapeutic target in the treatment of gastric cancer. PMID: 25815687
42. The up-regulation of FSCN1 is a prognostic factor in patients diagnosed with hepatocellular liver cancer. PMID: 26173501
43. Upregulated fascin expression was detected in melanoma metastases, but was not correlated to patient outcome. PMID: 25535872
44. miR-133b directly targeted FSCN1 which functioned as an oncogenic gene in gastric cancer cells. PMID: 25433493
45. Fascin-1 and paxillin were expressed in 58% and 43% of infiltrating duct carcinoma cases. There was a significant correlation between fascin-1 and paxillin expression and tumor grade, clinical stage, lymph-node metastasis grade, and HER2 expression. PMID: 26349603
46. The cortactin SH3 domain contributes to podosome assembly while fascin actin bundling is a master regulator of podosome disassembly in THP-1 macrophages and dendritic cells. PMID: 25601713
47. In ovarian primary mucinous tumors, fascin and EMMPRIN may play an important role in tumor progression from benign tumor to carcinoma. PMID: 25155376
48. miR-133a functions as a tumor suppressor and directly targets FSCN1 in pancreatic cancer. PMID: 25198665
49. LMP1-mediated upregulation of Fascin depends on NF-kappaB and both NF-kappaB and Fascin contribute to invasive migration of LMP1-expressing lymphocytes. PMID: 25105941
50. Fascin plays a role in stress fiber organization and focal adhesion disassembly. PMID: 24930964
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亞細胞定位：

Cytoplasm, cytosol. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell projection, filopodium. Cell projection, invadopodium. Cell projection, microvillus. Cell junction.
蛋白家族：

Fascin family
組織特異性：

Ubiquitous.
數據庫鏈接：

HGNC: 11148

OMIM: 602689

KEGG: hsa:6624

STRING: 9606.ENSP00000371798

UniGene: Hs.118400

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