ELAVL1 Recombinant Monoclonal Antibody

說明書

中文名稱：

ELAVL1 Recombinant Monoclonal Antibody
貨號：

CSB-RA555528A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: HEK293 whole cell lysate(30μg), U-251MG whole cell lysate(30μg), PC-3 whole cell lysate(30μg), HL-60 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), COLO205 whole cell lysate(30μg), HeLa whole cell lysate(30μg), Jurkat whole cell lysate(30μg)
All lanes: ELAVL1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 10s

IHC image of CSB-RA555528A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela cell with CSB-RA555528A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing MCF-7 cells stained with CSB-RA555528A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q15717
基因名：

ELAVL1
別名：

ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody; ELAV (embryonic lethal; abnormal vision; Drosophila) like 1 (Hu antigen R) antibody; ELAV like 1 antibody; ELAV like RNA binding protein 1 antibody; ELAV-like protein 1 antibody; ELAV1 antibody; ELAV1_HUMAN antibody; Elavl1 antibody; Embryonic lethal abnormal vision drosophila homolog like 1 antibody; Hu Antigen R antibody; Hu-antigen R antibody; Hua antibody; HuR antibody; MelG antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human ELAVL1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

11D12
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA. Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR.
基因功能參考文獻：
1. B4GALT1-AS1 recruited HuR to enhance YAP mRNA stability and thus its transcriptional activity PMID: 30182452
2. HuR associates with TERC in vitro and in cultured cells. The association of HuR with TERC was required for the maintenance of TERC methylation and hence telomerase activity. Additionally, the regulation of telomerase activity by HuR was found to impact on the renewal of hematopoietic stem cells and was linked to dyskeratosis congenita, aplastic anemia, and autosomal dominant dyskeratosis congenita. PMID: 29880812
3. this study identified an essential Jun/miR-22/HuR regulatory axis in CRC (the working model is summarised in Fig. 8) and highlighted the vital role of HuR and miR-22 in CRC proliferation and migration. PMID: 29351796
4. RNA immunoprecipitation and RNA pull-down assays in glioma cell lines demonstrated that SNHG12 was associated with and was stabilized by Hu antigen R. Glioma patients with high levels of SNHG12 exhibited a reduced 5-year overall survival rate. PMID: 30015836
5. Knockdown of HuR significantly increases the apoptosis of airway epithelial cells and down-regulates ZEB-1 expression. PMID: 29866111
6. High HUR expression is associated with Invasive Breast Carcinoma. PMID: 28808873
7. HuR acts as a tumor promoter by enhancing YAP expression in osteosarcoma cells. PMID: 29597092
8. Study supports HuR's role as an upstream regulator of p62 expression in ARPE-19 cells, helps to understand better the early events in response to a proautophagy stimulus. PMID: 29576851
9. Our data...strongly suggest that HuR expression...could be used as a prognostic marker in melanoma. PMID: 29393978
10. HuR protein is expressed in the majority of the cases in all the tumors of the head and neck region examined. More importantly, higher levels of HuR expression have been noted, showing higher cytoplasmic levels in malignant or premalignant cases. Moreover, HuR modulates several genes implicated in biological processes important for malignant transformation, growth, and invasiveness. [review] PMID: 29619127
11. Radiation-activated ataxia-telangiectasia mutated kinase/p38 signaling positively contributed to the nucleus to cytosol translocation of HuR. PMID: 29856906
12. that HuR is involved in the process of acquiring malignant histopathological features and ability to invade the muscularis propria by urothelial bladder cancer cells PMID: 29566946
13. HuR facilitated lung cancer stemness dependent on CDK3 expression. miR-873 or miR-125a-3p level was negatively correlated with HuR and CDK3 expression levels in lung cancer tissues. HuR facilitates lung cancer stemness via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis. PMID: 29344850
14. HuR plays an important role in the progression of esophageal carcinoma by targeting IL-18. PMID: 28231690
15. the promotive effects of HuR overexpression on the inflammatory response were attenuated when HUVECs were cotreated with HMGB1 short hairpin RNA. Therefore, the present results indicated that the ectopic expression of HuR may induce inflammatory responses and thus sepsis by activating the HMGB1 signaling pathway. PMID: 29115544
16. Computational analysis of publicly available microarray datasets of non-small cell lung cancer (NSCC) treated with EGFR targeted therapy identified ESR1 and ELAV1 as the most highly ranked hub genes involved in the NSCLC acquired TKI-resistance. PMID: 29758295
17. Studies indicate that by regulating the mRNAs of target genes, Hu antigen R protein (HuR) is causally linked to the onset and progression of kidney diseases [Review]. PMID: 28910975
18. The widespread distribution of intronic adenylate-uridylate (AU)-rich elements (AREs) and their particular association with ELAVL1/HuR and ELAVL1/HuR binding sites indicates that more than half of human genes can be regulated post-transcriptionally by AREs. PMID: 29413897
19. Posttranscriptional regulation of HuR by miR-133b enhances DTX cytotoxicity through inhibition of ABCG2. PMID: 29327946
20. HuR is required for pancreatic ductal adenocarcinoma tumor growth. PMID: 28242812
21. Transgenic expression of HuR in astrocytes accentuated neuronal injury and other secondary features of spinal cord injury including increased vascular permeability and astrocyte activation. PMID: 28487080
22. HuR may be involved in the pathogenesis of glaucoma. PMID: 29107807
23. Findings reveal a new mechanism whereby HUR protects NONO from mir320-mediated degradation upon UVC exposure and identify a new component within the complex network of players underlying the DNA damage response adding mir320a to the list of p53-regulated targets upon genotoxic stress. PMID: 27816966
24. ectopic expression of HuR promotes breast cancer cell proliferation and survival by directly binding to and stabilizing CDK3 mRNA. PMID: 28501005
25. New ELAVL1-TYK2 fusion gene is oncogenic in acute myeloid leukemia. PMID: 27189703
26. High expression of HuR is correlated with thyroid cancer. PMID: 27542231
27. Folate deficiency impaired spermatogenesis and reduced sperm concentration may partly due to inhibiting the expression of the three key molecules (Esr1, Cav1 and Elavl1) essential for sperm production. PMID: 28445960
28. these studies demonstrate that HuR knockdown in triple-negative breast cancer cells elicits oxidative stress and DNA damage resulting in radiosensitization PMID: 27588488
29. LncRNA-AK058003 can reduce the expression of mRNA stabilizing protein HuR and act as a precursor of miR-15a to suppress gamma-synuclein-mediated cell proliferation and the metastasis of hepatocellular carcinoma. PMID: 28035067
30. These results indicate that HuR plays an important role in adenovirus replication, at least in part, by up-regulating IVa2 mRNA expression and that AU-rich element (ARE)-mRNA stabilization is required for both transformation and virus replication. PMID: 29225167
31. Data indicate that both ELAVL1 and TIAR positively regulate endogenous SNCA in vivo. PMID: 29149290
32. identified HuR as a novel posttranscriptional regulator of ABCA1 expression and cellular cholesterol homeostasis, thereby opening new avenues for increasing cholesterol efflux from atherosclerotic foam macrophages and raising circulat-ing HDL cholesterol levels. PMID: 24729624
33. HuR expression and cytoplasmic localization were increased in fibrotic livers. S1P induced migration of human bone marrow Mesenchymal Stem Cells via S1PR3 and HuR. PMID: 27543493
34. RNA-binding protein HuR (HuR) expression negatively correlated with chemokine (CC motif) ligand 5 (CCL5) expression and macrophage appearance in a cohort of breast tumors. PMID: 28731284
35. Data indicate no correlation between RNA-binding protein HuR intracellular localization in tumor sections and progression free survival. PMID: 26943573
36. our research demonstrated that NEAT1 acts as a key regulator in human ovarian carcinogenesis and revealed two regulatory factors (HuR and miR-124-3p) of NEAT1 expression. PMID: 27075229
37. The crucial role of HuR as a stabilizing factor for the MR transcript. PMID: 28744670
38. study found cytoplasmic HuR is significantly overexpressed in higher-grade (atypical and anaplastic) than in lower-grade meningiomas and the HuR level is correlated with survival in patients with meningioma; siHuR-induced HuR knockdown was shown to reduce the growth of both Ben-Men-1 and IOMM-Lee cells PMID: 28493484
39. combined treatments of siHuR and epirubicin significantly reduced the expression of Bcl-2, but increased the expression of Bax, as well as activity and expression levels of caspase-3 and -9. In contrast, overHuR abrogated these effects PMID: 28968471
40. High HuR expression is associated with pancreatic ductal adenocarcinomas. PMID: 28687616
41. GPRC5A is a potential oncogene in pancreatic ductal adenocarcinoma cells that is upregulated by gemcitabine with help from HuR. PMID: 27415424
42. Hur role in the epithelial-to-mesenchymal transition in pancreatic cancer PMID: 28965949
43. exposing glioma cells to the HuR inhibitor tanshinone group compound 15,16-dihydrotanshinone-I or to the newly identified compound 5 disrupts HuR multimerization modules and reduces tumor cell survival and proliferation. PMID: 28790173
44. We propose that the extensive binding of CircPABPN1 to HuR prevents HuR binding to PABPN1 mRNA and lowers PABPN1 translation, providing the first example of competition between a circRNA and its cognate mRNA for an RBP that affects translation. PMID: 28080204
45. HuR contributes to efficient oxidative phosphorylation by regulating of CoQ10 biosynthesis. PMID: 26690054
46. Data (including data from studies using knockout mice or cells from knockout mice) suggest HUR post-transcriptionally regulates CCR6 expression by binding to and stabilizing CCR6 mRNA and by promoting CCR6 translation in helper T-cells; knock-out of HUR reduces CCR6 expression on helper T-cells, impairs their migration to CNS, and prevents experimental autoimmune encephalomyelitis. (CCR6 = C-C chemokine receptor type 6) PMID: 28684423
47. Taken together, our results indicate that HuR activation is an early event in familial adenomatosis polyposis (FAP)--adenoma but is not present in inflammatory bowel disease (IBD)-dysplasia. Furthermore, our results offer a preclinical proof of concept for HuR inhibition as an effective means of FAP chemoprevention, with caution advised in the setting of IBD PMID: 28428272
48. Negative regulation of RNA-binding protein HuR by tumor-suppressor ECRG2 PMID: 26434587
49. Data suggest that ERBB2 is post-transcriptionally up-regulated in tamoxifen-resistant estrogen receptor-positive breast cancer cells; ERBB2 expression is decreased post-transcriptionally by miRNA26a/b binding to its 3prime- untranslated region; ERBB2 expression is increased post-transcriptionally by HuR binding to its 3prime-untranslated region. (ERBB2 = epidermal growth factor receptor 2; HuR = Hu antigen R) PMID: 28637868
50. by modulating extracellular export of miR-122, HuR could control stress response in starved human hepatic cells. PMID: 27402548
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亞細胞定位：

Cytoplasm. Nucleus. Cytoplasm, Stress granule.
蛋白家族：

RRM elav family
組織特異性：

Ubiquitous. Detected in brain, liver, thymus and muscle.
數據庫鏈接：

HGNC: 3312

OMIM: 603466

KEGG: hsa:1994

STRING: 9606.ENSP00000385269

UniGene: Hs.184492

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