EEF1G Recombinant Monoclonal Antibody
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中文名稱：EEF1G Recombinant Monoclonal Antibody
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貨號：CSB-RA149810A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(20μg),Hela whole cell lysate(20μg), A549 whole cell lysate(20μg), COLO-205 whole cell lysate(20μg),HT-29 whole cell lysate(20μg),PC-3 whole cell lysate(20μg)
All lanes: eEF1B gamma antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time：20s -
Immunofluorescence staining of Hela cell with CSB-RA149810A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing BxPC-3 cells stained with CSB-RA149810A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產(chǎn)品詳情
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Uniprot No.：
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基因名：EEF1G
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別名：2610301D06Rik antibody; AA407312 antibody; eEF 1B gamma antibody; EEF 1G antibody; eEF-1B gamma antibody; EEF1G antibody; EF 1 gamma antibody; EF 1G antibody; EF-1-gamma antibody; EF1 gamma antibody; EF1G antibody; EF1G_HUMAN antibody; Elongation factor 1 gamma antibody; Elongation factor 1-gamma antibody; Eukaryotic translation elongation factor 1 gamma antibody; GIG 35 antibody; GIG35 antibody; MGC103354 antibody; MGC114210 antibody; MGC144723 antibody; MGC144724 antibody; MGC94929 antibody; Pancreatic tumor related protein antibody; PRO1608 antibody; Translation elongation factor eEF 1 gamma chain antibody
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反應種屬：Human
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免疫原：A synthesized peptide derived from human EEF1G
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：6F1
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應用范圍：ELISA, WB, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
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靶點詳情
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功能：Probably plays a role in anchoring the complex to other cellular components.
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基因功能參考文獻：
- Results show that eEF1Bgamma binds not only Che-1 and p53 transcripts but also their promoters. Its depletion significantly decreases p53 protein accumulation and slightly impacts on Che-1 accumulation in the mitochondria. These data support the notion that eEF1Bgamma, besides its canonical role in translation, is an RNA-binding protein and a key player in cellular stress responses. PMID: 27639846
- eEF1gamma, in addition to its role in translation elongation complex, is involved in regulating Vimentin gene by contacting both pol II and the Vimentin promoter region and shuttling/nursing the Vimentin mRNA PMID: 21217813
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組織特異性：Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver.
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數(shù)據(jù)庫鏈接：
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