EBI3 Recombinant Monoclonal Antibody

說明書

中文名稱：

EBI3重組抗體
貨號：

CSB-RA256644A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: JK whole cell lysate
All lanes: EBI3 antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA256644A0HU EBI3重組單克隆抗體是經(jīng)ELISA和Western Blot驗證的高特異性科研工具。該抗體靶向EBI3（Epstein-Barr病毒誘導(dǎo)基因3），該蛋白作為IL-12細(xì)胞因子家族成員，通過與IL-27α或p28亞基形成異源二聚體IL-27/IL-35，在調(diào)節(jié)T細(xì)胞分化、抗感染免疫及自身免疫平衡中發(fā)揮關(guān)鍵作用。實驗數(shù)據(jù)顯示，該抗體在Western Blot中可有效識別天然及重組EBI3蛋白，經(jīng)多批次驗證呈現(xiàn)清晰單一目標(biāo)條帶，推薦工作濃度為1:500-1:2000，并采用含BSA的穩(wěn)定緩沖體系確保檢測一致性。其高靈敏度和低交叉反應(yīng)特性使其適用于探究EBI3在免疫調(diào)節(jié)通路中的分子機(jī)制、Th細(xì)胞功能研究、感染性疾病模型構(gòu)建以及自身免疫相關(guān)蛋白互作分析等領(lǐng)域，為炎癥性疾病、腫瘤微環(huán)境等基礎(chǔ)研究提供可靠檢測方案。
Uniprot No.：

Q14213
基因名：

EBI3
別名：

Interleukin-27 subunit beta (IL-27 subunit beta) (IL-27B) (Epstein-Barr virus-induced gene 3 protein) (EBV-induced gene 3 protein), EBI3, IL27B
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from Human EBI3
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

12H5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
基因功能參考文獻(xiàn)：
1. The IL-35 levels in CagA(+) H. pylori-infected participants from peptic ulcer and H. pylori-infected asymptomatic groups were lower than individuals infected with CagA(-) strains. PMID: 29938865
2. IL-35 may be involved in the pathogenesis of Primary biliary cirrhosis. PMID: 29445068
3. IL-35 is a relatively newly discovered member of the IL-12 family which are unique in structure as they are dimer formed by two subunits; recent findings have shown abnormal expression of IL-35 in inflammatory autoimmune diseases and functional analysis suggested that IL-35 is critical in the onset and development of these diseases. [Review] PMID: 29729445
4. IL-35 expression was significantly increased in patients with chronic hepatitis C and was positively correlated with the levels of viral RNA PMID: 28644966
5. Pancreatic ductal carcinoma cells produce IL35 to recruit monocytes via CCL5 and induce macrophage to promote angiogenesis via expression of CXCL1 and CXCL8. PMID: 28989066
6. The study revealed that post-therapeutic recovery of circulating IL-35 concentration might be an independent predictor for effective response to IST in pediatric AA. PMID: 28211781
7. The plasma levels of interleukin-35 were significantly higher in the hepatocellular carcinoma patients than the controls. PMID: 27699510
8. this study introduces IL-35 as a new treatment for pemphigus PMID: 27855302
9. over-expression of EBI3 could reduce the apoptosis of Treg/CD4(+)T/CD8(+)T cells and prevent radiation-induced immunosuppression of cervical cancer HeLa cells by inhibiting the activation of PD-1/PD-L1 signaling pathway PMID: 28351328
10. Data show that interleukin-35 (Epstein-Barr virus-induced gene 3 (EBI3) and the interleukin-12 Subunit p35 (p35) subunit) levels were significantly elevated in the patients with influenza infection. PMID: 26844658
11. higher decidual mRNA expression in preeclampsia PMID: 26472010
12. IL-35 was elevated in bone marrow of adult AML patients and this increase was correlated with the clinical stages of malignancy, suggesting that IL-35 is involved in pathogenesis of AML. PMID: 26431888
13. Elevated circulating IL-35, particularly at early phase, its decrease after treatment initiation, and a positive association between synovial fluid IL-35 and disease activity support an involvement of IL-35 in the pathogenesis of RA. PMID: 26204444
14. Data suggest that the toll like receptor 3 (TLR3)-interferon regulatory factor 6 protein (IRF6)-interleukin-23 subunit p19 (p19)/EBI3 protein axis may regulate keratinocyte functions in the skin. PMID: 26303210
15. EBI3 gene rs4740 polymorphism is closely associated with susceptibility to pulmonary tuberculosis and the elevation and enrichment of EBI3 in the lung derived from macrophages may contribute to the exacerbation of mycobacterial infection. PMID: 25937126
16. EBI3 Downregulation Contributes to Type I Collagen Overexpression in Scleroderma Skin. PMID: 26355156
17. IL-35 mRNA and protein were higher in tuberculous pleural effusions than in malignant ones. PMID: 25935866
18. IL-35 can effectively suppress the proliferation and IL-4 production of activated CD4+CD25- T cells in allergic asthma, and that IL-35 may be a new immunotherapy for asthma patients. PMID: 26044961
19. IL-35 is highly expressed in chronic HBV CD4(+) T-cells and plays an important role in the inhibition of the cellular immune response in chronic HBV. PMID: 25869609
20. The levels of EBI3 and IL-12p35 mRNAs in peripheral blood mononuclear cells in moderate or hyper-responders were significantly higher than those in non- or hypo-responders. PMID: 25575066
21. The results suggest that the decreased expression of IL-35 could be involved in the pathogenesis of childhood asthma. PMID: 24970690
22. IL-17 and IL-35 may be critically involved in the pathogenesis of hepatitis B-related LC. PMID: 25323532
23. IL35 appears to contribute to the loss of immunological self-tolerance in ITP patients by modulating T cells and immunoregulatory cytokines. PMID: 25640666
24. IL-35 levels are dramatically decreased in immune thrombocytopenia patients, suggesting that IL-35 may be involved in the pathogenesis of this disease. PMID: 24994465
25. Interleukin-35 induces regulatory B cells that suppress autoimmune disease. [il-35] PMID: 24743305
26. ingestion of apoptotic cells by DCs leads to increased expression of IL-12p35 and Ebi3 without affecting IL-12p40. PMID: 24782489
27. circulating IL-35 in PDAC patients significantly increased, suggesting that regulating the expression of IL-35 may provide a new possible target for the treatment of PDAC patients, especially for the resectable ones. PMID: 24121041
28. The increased expression of IL-35 in chronic and aggressive periodontitis suggests its possible role in pathogenesis of periodontitis. PMID: 24376289
29. EBI3-overexpression in MRL/lpr mice induces generation of regulatory T cells, causing suppression of autoimmune and inflammatory reactions by affecting the T helper (Th)1 cell/Th2 cytokine balance. PMID: 23845089
30. in contrast to TGF-beta, IL-35 is not constitutively expressed in tissues but it is inducible in response to inflammatory stimuli PMID: 22438968
31. the findings from the past decade identify IL-27 as a critical immunoregulatory cytokine, especially for T cells, whereas some controversy is fueled by results challenging the view of IL-27 as a classical silencer of inflammation. PMID: 23904441
32. expressed by trophoblast cells PMID: 23619469
33. The findings of this study suggest that SNPs in FOXP3 and EBI3 genes modify the risk for development of chronic rhinosinusitis. PMID: 23562195
34. these results reveal a novel functional role for IL-35 in suppressing cancer activity, inhibiting cancer cell growth, and increasing the apoptosis sensitivity of human cancer cells through the regulation of genes related to the cell cycle and apoptosis. PMID: 23154182
35. The findings of this study support the potential role of regulatory T cells and genetic variations in the regions around FOXP3 and EBI3 genes in modifying the risk for AR development in Chinese patients. PMID: 22836044
36. This study demonstrated that interleukin-35 expression could be detected in the CD4(+) T cells from peripheral blood of chronic hepatitis B patients. PMID: 21285006
37. IL-27 expression is one host immune factor produced in response to influenza A virus infection and that elevated IL-27 levels inhibit viral replication. PMID: 22343630
38. Data show that Epstein-Barr virus-induced gene 3 (EBI3) is differentially expressed among Burkitt lymphoma and diffuse large B-cell lymphoma. PMID: 21931777
39. a new heterodimeric cytokine that consists of EBI3, an IL-12p40-related protein, and p28, a newly discovered IL-12p35-related polypeptide (IL-27) PMID: 12121660
40. results suggest that increased numbers of Epstein-Barr virus-infected cells in areas of active inflammatory bowel disease are secondary to influx or local proliferation of inflammatory cells & do not contribute significantly to local production of EBI3 PMID: 15170639
41. findings indicate that the restricted Th1 responses in newborns owing to deficient IL-12 production may be compensated for, in part, by enhanced IL-27 secretion PMID: 18167155
42. The genome-wide mRNA expression profile under the condition of short-term stimulation (4h) with IL-18 using the Affymetrix GeneChip((R)) Array System, was characterized. PMID: 18336908
43. Our data support a possible role of Ebi3 in atherogenesis PMID: 19556516
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Type I cytokine receptor family, Type 3 subfamily
數(shù)據(jù)庫鏈接：

HGNC: 3129

OMIM: 605816

KEGG: hsa:10148

STRING: 9606.ENSP00000221847

UniGene: Hs.501452

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