CTNNA1 Recombinant Monoclonal Antibody

說明書

中文名稱：

CTNNA1 Recombinant Monoclonal Antibody
貨號：

CSB-RA028796A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate(20μg),HT-29 whole cell lysate(20μg),COLO-205 whole cell lysate(20μg),Hela whole cell lysate(20μg),A549 whole cell lysate(20μg),HEK293T whole cell lysate(20μg)
All lanes: alpha Catenin antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 100 kDa
Observed band size: 115 kDa
Exposure time：20s

Overlay Peak curve showing MCF-7 cells stained with CSB-RA028796A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P35221
基因名：

CTNNA1
別名：

Alpha E-catenin antibody; Cadherin associated protein 102kDa antibody; Cadherin associated protein antibody; Cadherin-associated protein antibody; CAP 102 antibody; CAP102 antibody; Catenin (cadherin associated protein) alpha 1 102kDa antibody; Catenin (cadherin associated protein), alpha 1, 102kDa antibody; Catenin alpha 1 antibody; Catenin alpha-1 antibody; CTNA1_HUMAN antibody; CTNNA 1 antibody; Ctnna1 antibody; FLJ36832 antibody; FLJ52416 antibody; MDPT2 antibody; NY REN 13 antigen antibody; OTTHUMP00000224141 antibody; OTTHUMP00000224147 antibody; Renal carcinoma antigen NY REN 13 antibody; Renal carcinoma antigen NY-REN-13 antibody
反應種屬：

Human, Mouse
免疫原：

A synthesized peptide derived from human CTNNA1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

2B7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. May play a crucial role in cell differentiation.
基因功能參考文獻：
1. These findings provide mechanistic insight into the WNT-mediated regulation of the DNA damage response and suggest a novel role for the alpha-catenin-beta-catenin complex in the nucleus. PMID: 28348105
2. pseudogene CTNNAP1 is a potential tumor suppressor participating in CRC pathogenesis by competing with the parent gene CTNNA1 for microRNA-141. PMID: 27487124
3. Hypermethylation of the CTNNA1 promoter was associated with unfavorable karyotype, and possessed the higher frequency of coexisting with ASXL1 and RUNX1 mutations. PMID: 27129146
4. The results of this study demonstrate that causative variants identified in the CTNNA1 and CYP4V2 genes are also associated with Leber Congenital Amaurosis. PMID: 28453600
5. alpha- and beta-catenins may be important in the early stages of phyllodes tumours development, while E-cadherin may be required for malignant development PMID: 22571452
6. This work identified alpha-catenin as another molecule in addition to E- and P-cadherin that were targeted to inactivate homotypic cell-in-cell structures formation in human tumor cells. PMID: 26192076
7. Progressive loss of e-cadherin/alpha-catenin expression is associated with an aggressive phenotype (low differentiation, increased metastatic activity/advanced stage) in thyroid carcinomas. PMID: 27273957
8. study identifies CTNNA1 gene variants as a cause of macular dystrophy, indicates that CTNNA1 is involved in maintaining RPE integrity PMID: 26691986
9. The results demonstrate a Fas-mediated apoptotic signaling pathway that is enhanced by the age-dependent loss of alpha(E)-catenin in renal tubule epithelial cells. PMID: 25894537
10. Actin-dependent CTNNA1 clustering is a unique molecular mechanism mediating both integrity and reassembly of the cell-cell adhesive interface formed through weak cis- and trans-intercadherin interactions. PMID: 26261181
11. alpha-catenin is a reversible, stretch-activatable sensor that mechanically links cadherin complexes and actin and is an indispensable player in cadherin-specific mechanotransduction at intercellular junctions. PMID: 25544608
12. alpha-catenin functions as a tumor suppressor in E-cadherin-negative basal like breast cancer cells by inhibiting NF-kappaB signaling. PMID: 25483184
13. CTNNA1 hypermethylation was detected in three out of four with isolated del(5q), one with trisomy 11, one with monosomy 7, one out of four with del(20q), and one out of seven with complex abnormalities, but in none with trisomy 8. PMID: 25153418
14. CTNNA1 methylation is a recurrent event but has no influence on prognosis in acute myeloid leukemia PMID: 24685333
15. CTNNA1 expression is specifically downregulated in basal-like breast cancer subtype, correlates with clinical outcome and inversely correlates with TNF and RELB expression. PMID: 24509793
16. A germline truncating allele of alpha-E-catenin (CTNNA1) was identified that was present in two family members with invasive diffuse gastric cancer and four in which intramucosal signet ring cells were detected as part of endoscopic surveillance. PMID: 23208944
17. A discrete trimeric complex of beta-catenin, alpha-catenin and the tumor suppressor APC, forms in the cytoplasm in response to Wnt signaling. PMID: 22469663
18. Data show that E-cadherin and alpha-catenin were predominantly expressed in the cell membranes, whereas beta- and gamma-catenin were found both in the cell membrane and cytoplasm. PMID: 20933443
19. The expressions of E-cd and alpha-cat are significantly lower in prostate cancer than in benign prostatic hyperplasia, and they are not associated with cancerous metastasis, but negatively correlated with the PSA level in PCa patients. PMID: 22774602
20. study presents evidence that in the cadherin-catenin complex alpha-catenin contributes to the binding strength of another catenin, p120, to the same complex; data suggest alpha-catenin-p120 contact within the cadherin-catenin complex can regulate cadherin trafficking PMID: 21937720
21. binding studies suggest that vinculin must be in an activated state to bind to alpha-catenin and that this interaction is stabilized by the formation of a ternary alpha-catenin-vinculin-F-actin complex, which can be formed via the F-actin binding domain PMID: 22493458
22. Report loss of CTNNA1 as lobular carcinoma in situ of the breast progresses to more invasive lesions. PMID: 22080244
23. Data demonstrate that the expression of alpha-E-catenin is increased by Sec6 siRNAs, and E-cadherin and beta-catenin localize mainly at the cell-cell contact region in HSC3 cells, which were transfected with Sec6 siRNA. PMID: 22381337
24. The expression of alpha-catenin was significantly lower in the invasive group than in the non-invasive group of pituitary adenoma. PMID: 16927799
25. These results suggest that JNK affects the association of alpha-catenin with the adherens junction complex and regulates adherens junctions. PMID: 21030692
26. 4 biallelic inactivating alpha-catenin mutations were found among 55 human breast cancer cell lines. All caused premature termination. Loss of alpha-catenin protein expression was associated with the lobular subtype in primary breast cancers. PMID: 19763817
27. The E-cadherin-catenin complex is the factor indicative of metastasis and disease progression in gastric cancer. PMID: 20529814
28. The study objective was to assess changes in the expressions of E-cadherin and alpha-, beta- and gamma-catenin proteins in pancreatic duct carcinoma in correlation with clinicopathological parameters, lymph node involvement and distant metastases. PMID: 20529828
29. Data show that actin bundle formation and subsequent linkage between actin bundles and VE-cadherin through alpha- and beta-catenins are important for the stabilization of VE-cadherin at the cell-cell contacts in cAMP-Epac-Rap1 signal-activated cells. PMID: 20032304
30. An evolutionarily conserved PTEN-C/EBPalpha-CTNNA1 axis controls myeloid development and transformation. PMID: 20371743
31. results show that the association of alpha-catenin with the cadherin-catenin complex is required for efficient leukocyte transendothelial migration PMID: 19918298
32. Abnormal E-cadherin and alpha-catenin and beta-catenin in pancreatic carcinoma tissues. Abnormal E-cadherin and alpha-catenin with differentiation, lymph node and liver metastases. Aberrant beta-catenin with lymph node and liver metastases. PMID: 12532469
33. Pancreatic cancer likely occurs in case of the inactivation of E-cadherin and alpha-catenin genes and abnormal expression of proteins PMID: 14599963
34. alpha-catenin has a role in cell growth control in three-dimensional culture PMID: 14755240
35. Allelic imbalance occurs at two distinct regions of which one includes the CTNNA1 gene in ovarian cancer. PMID: 15297182
36. Our results suggest that alpha-catenin links CCR5 and CXCR4 to the cytoskeleton and is involved in the organization of these receptors at the membrane, thereby possibly affecting HIV-1 infection. PMID: 15541354
37. ubiquitin-independent degradation of alpha-catenin regulates beta-catenin signaling and maintenance of the differentiated phenotype of articular chondrocytes PMID: 15695815
38. Loss of expression in squamous cell carcinoma of the floor of the mouth correlated significantly with poor prognosis PMID: 15916880
39. Downregulation of alpha-catenin expression is common in gastric carcinoma PMID: 15948257
40. These results indicate that the interaction of alpha-catenin and actin functions in the assembly of desmosomes in epithelial cells. PMID: 16273278
41. Cdc42 regulates AJ permeability by controlling the binding of alpha-catenin with beta-catenin and the consequent interaction of the VE-cadherin/catenin complex with the actin cytoskeleton. PMID: 16322481
42. Direct attachment of alpha-catenin to F-actin is required to promote cadherin-mediated contact formation and strong cell-cell adhesive states. PMID: 16798615
43. we explored the implication of three proteins (E-cadherin, a- and b-catenins) that form the cadherin-catenin complex, a receptorial structure strictly involved in tumoral vascular invasion and embolization in this biologic event PMID: 17576040
44. The previously reported characteristics of this mutation, E-cadherin (V832M) do not apply to human epithelial cells expressing this mutant protein. PMID: 17668349
45. prognostic and chemosensitivity marker for invasive bladder cancer PMID: 17760743
46. removal of N-glycans on E-cadherin resulted in elevated tyrosine phosphorylation level of beta-catenin and reduced beta- and alpha-catenins at adherens junctions PMID: 17979184
47. These results suggest induction of SRF-mediated transcription by alpha(E)-catenin either downstream of RhoA or via a parallel pathway. PMID: 18078809
48. Rsults suggest that the lower epithelial alpha-catenin, E-cadherin and (or) ZO-1 expression in patients with atopic asthma contributes to a defective airway epithelial barrier and a higher influx of eosinophils in the epithelium. PMID: 18418437
49. This study reveals for the first time that alpha-catenin is a key regulator of beta-catenin transcriptional activity and that the status of alpha-catenin expression in tumor tissues might have prognostic value for Src targeted therapy PMID: 18566211
50. The mean values of the percentage of positive cells for the tested proteins between E-AD vs. AD did not demonstrate any statistically significant difference except for alpha-catenin. PMID: 19124205
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相關疾病：

Macular dystrophy, patterned, 2 (MDPT2)
亞細胞定位：

[Isoform 1]: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Note=Found at cell-cell boundaries and probably at cell-matrix boundaries.; [Isoform 3]: Cell membrane; Peripheral membrane protein; Cytoplasmic side.
蛋白家族：

Vinculin/alpha-catenin family
組織特異性：

Expressed ubiquitously in normal tissues.
數據庫鏈接：

HGNC: 2509

OMIM: 116805

KEGG: hsa:1495

STRING: 9606.ENSP00000304669

UniGene: Hs.445981

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