CKB Recombinant Monoclonal Antibody
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中文名稱：CKB Recombinant Monoclonal Antibody
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貨號：CSB-RA936751A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: HEK293 whole cell lysate(30μg),Hela whole cell lysate(30μg), PC-3 whole cell lysate(30μg),Mouse brain tissue lysate(30μg),Rat brain tissue lysate(30μg)
All lanes: Creatine kinase B type antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 43 kDa
Observed band size: 48 kDa
Exposure time：2min -
Overlay Peak curve showing Hela cells stained with CSB-RA936751A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：CKB
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別名：B CK antibody; B-CK antibody; BB-CK antibody; BCK antibody; Brain creatine kinase antibody; Ckb antibody; CKBB antibody; Creatine kinase B antibody; Creatine kinase B chain antibody; Creatine kinase B type antibody; Creatine kinase B-type antibody; Creatine Kinase BB Isoenzyme antibody; Creatine kinase brain antibody; Creatine kinase brain type antibody; Creatine phosphokinase BB antibody; Epididymis luminal protein 211 antibody; Epididymis secretory protein Li 29 antibody; HEL 211 antibody; HEL S 29 antibody; KCRB_HUMAN antibody
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反應種屬：Human, Mouse, Rat
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免疫原：A synthesized peptide derived from human CKB
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：3E4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work.
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基因功能參考文獻：
- The data revealed a varying but significant increase of CK activity in CKBE individuals as compared to controls, reaching an almost 800-fold increase in two CKBE individuals which also had increased erythrocyte creatine. PMID: 28364583
- Association and oligomerization of Prx II could take part in recovery and protection of the CK BB enzyme activity from inactivation during heat-induced stress. PMID: 29227081
- membrane localization of BCK seems to be an important and regulated feature for the fueling of membrane-located, ATP-dependent processes, stressing again the importance of local rather than global ATP concentrations. PMID: 27318991
- These findings support increased CK activity as protection against ischaemia-reperfusion injury, in particular, protection via CKMT2 in a cardiac-relevant cell line, which merits further investigation in vivo. PMID: 28806770
- SRC, LYN and CKB expression or DNA methylation could be useful markers for predicting tumor progression. PMID: 26460485
- CK-MB levels were higher after ERCP in non-ischemic patients compared with a myocardial ischemia group. Creatine phosphokinase levels did not differ significantly between groups. PMID: 25141318
- CK-B catalytic activity also helps in the formation of protrusive ruffle structures which are actin-dependent and abundant on the surface of both unstimulated and LPS-activated macrophages. PMID: 25538032
- increased serum Ckbb reflects failure of osteoclasts or suppression of osteoclasts in children with osteogenesis imperfecta during neridronate treatment PMID: 24518563
- Estimation of CK and its CK isoenzyme fractions can aid in quick and accurate diagnosis of tubal ectopic pregnancy. PMID: 23876027
- effects of osmolytes on human brain-type creatine kinase folding PMID: 22885020
- We found that most patients with macular telangiectasia-2 possess retinal autoantibodies, the most prevalent of which were directed against AGL, RBP3, and CK-B. PMID: 23882694
- analysis of SNPs and their effect on creatine kinase structure and function PMID: 23049898
- study found promoter SNPs of CKB and TPI1 were weakly associated with schizophrenia;in addition, IFNG polymorphisms were associated with schizophrenia; results suggest that IFNG and proteins affected by IFNG may play a role in the pathogenesis of schizophrenia PMID: 22623148
- de-methylated CKB gene is inherited that leads to high CKB expression levels in myeloic precursor cells in the bone marrow PMID: 22088263
- His103 and Phe107 in hASB9-2 are essential for binding to CKB. PMID: 22418839
- Cigarette smoke induced carbonylation and subsequent degradation of creatine kinase B are involved in the regulation of senescence in bronchial epithelial cells. PMID: 21980054
- The results suggested that the intra- and inter-subunit domain interactions modified the behavior of kinetic refolding. PMID: 21931810
- Data demonstrate that the downregulation of CKB may play an important role in colon cancer progression by promoting EMT. PMID: 21308735
- CKB was up-regulated in women older than 38 years, and its expression in cumulus cells was associated with embryo quality PMID: 20721618
- These observations suggested that the ability to generate the oxidized form could protect BBCK against the intracellular oxidative stress. PMID: 20923681
- Report the effects of prior calcium channel blocker therapy on creatine kinase-MB (myocardial form) levels after percutaneous coronary interventions. PMID: 19337554
- A single troponin I value at 3 days from symptom onset is a better predictor of infarct size compared to peak values and CK-MB. PMID: 20588136
- Phosphocreatine metabolism in the normal appearing white matter in multiple sclerosis is impaired due to decreased CK-B levels. PMID: 20520825
- The correlation of CK-BB as a disease biomarker in both CNS and peripheral tissues from Huntington's disease mice and patients may provide a powerful means to assess disease progression and prognosis. PMID: 20460152
- 2D fingerprinting & mass spectrometry reveal specific targets of protein oxidation in Alzheimer's disease brain, including creatine kinase BB, suggesting involvement of oxidatively modified proteins in neurodegeneration. PMID: 12160938
- Expression of CKB mRNA and CK-B sometimes occurred in blastic transformation of the hematopoietic system PMID: 15996648
- CKB was expressed in 78% of colon tumors PMID: 16424007
- GM130 and BB-CK co-localize specifically in a transient fashion during early prophase of mitosis, when GM130 plays an important role in Golgi fragmentation that starts also at early prophase. PMID: 17036164
- The asymmetric unit contained two molecules of CKB, giving a crystal volume per protein mass (Vm) of 1.80 A3 Da-1 and a solvent content of 31.6%. PMID: 18309274
- Autoantibodies to EsteD and BB-CK produced in experimental autoimmune uveoretinitis -induced mice were also detected in some endogenous uveitis patients, suggesting that these proteins might be autoantigens. PMID: 18552983
- Using a yeast 2-hybrid it was discovered that the C-terminal domain of KCC3, that is lost in most hereditary motor and sensory neuropathy with agenesis of the corpus callosum-causing mutations, directly interacts with brain-specific creatine kinase. PMID: 18566107
- The Tat-CK fusion protein markedly increased endogenous CK activity levels within PC12 cells. PMID: 18682038
- Three structural aspects of human-brain-type-creatine-kinase were identified by X-ray crystallography: the ligand-free-form at 2.2A; the ADP-Mg2+, nitrate, and creatine complex (transition-state-analogue complex; TSAC); and the ADP-Mg2+-complex at 2.0A. PMID: 18977227
- Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19165527
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亞細胞定位：Cytoplasm, cytosol. Mitochondrion.
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蛋白家族：ATP:guanido phosphotransferase family
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數據庫鏈接：
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