CASP9 Recombinant Monoclonal Antibody

說明書

中文名稱：

CASP9重組抗體
貨號：

CSB-RA940979A0HU
規格：

￥1320
圖片：
Immunofluorescence staining of HepG2 with CSB-RA940979A0HU at 1:7, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 525-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing HepG2 cells stained with CSB-RA940979A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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靶點詳情

產品詳情

產品描述：

CASP9 Recombinant Monoclonal Antibody（CSB-RA940979A0HU）靶向細胞凋亡關鍵執行因子半胱天冬酶-9（Caspase-9），該蛋白通過線粒體途徑調控程序性細胞死亡，在DNA損傷應答、發育調控及腫瘤發生等過程中發揮核心作用。本抗體采用重組表達技術制備，具有高批次一致性，經ELISA、免疫熒光（IF）和流式細胞術（FC）嚴格驗證，可特異性識別天然及活化狀態下的CASP9蛋白。推薦實驗稀釋比為IF 1:50-1:200、FC 1:50-1:200，實驗數據顯示其在亞細胞定位分析和細胞表面標記檢測中呈現清晰的陽性信號與低交叉反應性。適用于研究細胞凋亡通路調控機制、化療藥物敏感性評估、基因編輯模型驗證等科研領域，特別在腫瘤細胞死亡誘導實驗、神經退行性疾病模型構建及發育生物學研究中具有重要應用價值。該產品為凍干粉劑型，建議根據實驗體系優化復溶濃度，并配合陽性對照以確保結果可靠性。
Uniprot No.：

P55211
基因名：

CASP9
別名：

Caspase-9 (CASP-9) (EC 3.4.22.62) (Apoptotic protease Mch-6) (Apoptotic protease-activating factor 3) (APAF-3) (ICE-like apoptotic protease 6) (ICE-LAP6) [Cleaved into: Caspase-9 subunit p35, Caspase-9 subunit p10], CASP9, MCH6
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human CASP9
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

8G5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IF, FC

推薦稀釋比：

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
基因功能參考文獻：
1. CASP9 mutations cause recurrent folate-resistant neural tube defects. PMID: 29358613
2. Study described rare mutations of the apoptosis gene CASP9 identified in neural tube defect cases and demonstrated that the p.Y251C variant impairs the protein's apoptotic function, suggesting it is a loss-of-function variation; also showed that the p.R191G variant inhibited apoptosis under folate-deficient condition, highlighting the effect of gene-environment interactions in this complex disease. PMID: 29365368
3. these results revealed that caspase9 and activated caspase3 predominantly regulates cell apoptosis in human dental pulp stem cells from deciduous teeth. PMID: 29845240
4. Low CASP9 expression is associated with Colorectal Cancer. PMID: 29801534
5. the results of the present study suggest that miR-96-5p, which is frequently upregulated in hepatocellular carcinoma (HCC), inhibits apoptosis by targeting CASP9. Therefore, miR-96-5p may be a potential therapeutic target for HCC PMID: 29658604
6. CASP9 germline mutations may have played a role at least in part to the susceptibility of development of gliomas in a Li-Fraumeni-like family lacking a TP53 germline mutation. PMID: 27935156
7. The caspase 9 level was significantly lower and related with oxidant status in patients with polycystic ovary syndrome, while the circulating levels of caspases 3 and 7 were statistically similar in both PCOS and control groups. PMID: 27899026
8. Results indicate that the apoptotic protease-activating factor 1 (Apaf-1) apoptosome activates caspase-9 in part through sequestration of the inhibitory caspase recruitment domains (CARDs) domain. PMID: 28143931
9. DES1 plays a key role in palmitic acid-mediated caspase 9 and caspase 3 activation. PMID: 27364952
10. CASP9 expression associated with inhibition of miR-182. PMID: 28298075
11. CASP-9 polymorphism is associated with Primary Brain Tumors. PMID: 28870924
12. High CASP9 expression is associated with Lung Tumorigenesis. PMID: 27197231
13. knockdown of HMGI-C led to the significant induction of apoptosis via mitochondrial pathway by inducing miR34a and cell cycle arrest in MDA-MB-468 cells in vitro. PMID: 27245202
14. Results suggest that the formation of the apoptosome accompanied by the activation of caspase-9 may occur in brains affected by multiple system atrophy (MSA), and that a mitochondria-dependent apoptotic pathway may be partially associated with the pathogenesis of MSA. PMID: 27345387
15. Using recombinant proteins, this study investigated the influence of survivin on the inhibition of caspase-9 by XIAP in vitro. With a fluorescence-based assay for the apoptosome-stimulated activity of caspase-9, the study shows that survivin has no effect on the inhibition of caspase-9 by XIAP, neither in the presence nor in the absence of Smac. PMID: 27865841
16. Data show that caspase 9 (CASP9) single nucleotide polymorphism (rs1052576) TT genotype was seemed to be associated with higher risk of pathological stage. PMID: 28358701
17. The content of caspase 9 gene transcripts in peripheral blood leukocytes and plasma level of TNF-alpha were significantly higher in healthy subjects carrying C allele than in carriers of GG genotype. PMID: 28091912
18. Results show that mRNA and protein levels of HAX-1 in prostate cancer cell lines were significantly higher and inhibits cell apoptosis through caspase-9 inactivation. PMID: 26323553
19. Renal CASP9 expression is increased in diabetes and increased as diabetic nephropathy progressed. PMID: 27141571
20. Inhibition of Caspase-9 restricted, while Apaf-1 promoted, Chlamydia pneumoniae infection in HEp-2, HeLa, and mouse epithelial fibroblast (MEF) cells. PMID: 26290316
21. Levels of caspase-9, caspase-10, MAVS, and pIRF7 in mononuclear cells and the disease activity index (SLEDAI) in the systemic lupus erythematosus patients were determined. PMID: 25370148
22. Expression of mutant caspase-9 correlated with a downregulation of BAFFR (B-cell-activating factor belonging to the TNF family (BAFF) receptor) in B cells and ICOS (inducible T-cell costimulator) in T cells. PMID: 25569260
23. caspase-9 mediates Puma activation to determine the threshold for overcoming chemoresistance in cancer cells. PMID: 25356864
24. we suggest that phosphorylation of caspase-9 may be a useful tool to assess the state of gastrointestinal cancer and the effects of anti-cancer therapy. PMID: 25031754
25. DNA fragmentation, DNA damage, caspase-9 activation and a large increase in the sub-G1 and S cell cycle phases confirmed the occurrence of apoptosis in a time-dependent manner. PMID: 24377517
26. Data indicate a significant association of two single nucleotide polymorphism (SNPs) in aspase9 (CASP9) and two haplotypes of the four SNP combinations with acute myeloid leukemia (AML) susceptibility. PMID: 24879622
27. silica and double-stranded RNA (dsRNA) synergistically induces caspase-9-dependent apoptosis, but not inflammasome activation, of bronchial epithelial cells PMID: 24661197
28. that KAT5 RNAi may result in cleaved casp9 upregulation through p38MAPK activation in Gallbladder carcinoma cells PMID: 24427328
29. overexpression of iASPP and the low expression of caspase-9 in esophageal cancer are closely correlated with tumor invasion and metastasis PMID: 24405603
30. the Atg7.caspase-9 complex performs a dual function of linking caspase-9 to the autophagic process while keeping in check its apoptotic activity. PMID: 24362031
31. The inhibitory effect of Ab42 on the apoptotic pathway is associated with its interaction with procaspase-9 and consequent inhibition of Apaf-1 apoptosome assembly PMID: 24424093
32. The rs4645981 T allele and the rs4645981 T allele carrier might increase the risk of cancer, but the rs1052576 A allele, rs1052576 A carrier, rs2308941 T allele, and rs2308941 T carrier might be protective. PMID: 23479167
33. Interactions of caspase-9 with the BIR3 (baculovirus inhibitory repeat 3) exosite are essential for high-affinity binding. PMID: 23203690
34. In oligozoospermia, spermatogonia presented significant increased active caspase-9 PMID: 23359247
35. The change of caspase-9 expression from colon mucosa, adenoma to cancer suggested it may be involved in the carcinogenesis of colon cancer. PMID: 24592539
36. dCas9 effectors can exert positive or negative regulation on the expression of developmentally relevant genes, which can influence cell differentiation status when impinging on a key node in the regulatory network that governs the cell state. PMID: 24346702
37. BIRC5-31CC and CASP9+83CT/TT genotypes were associated with an increased risk for renal cell carcinoma development in the female group of our southern European study population. PMID: 23645041
38. OSU-03012 induces apoptosis in human esophageal carcinoma cells through a p53/Bax/cytochrome c/caspase-9-dependent pathway. PMID: 23652278
39. EGCG, both alone and in combination with cisplatin, promoted the expression of the pro-apoptotic splice isoform of caspase 9 PMID: 23615977
40. The results demonstrated that 50 microg/mL beta-glucan significantly repressed the expression of the ERCC5 gene, no change in CASP9 expression, and induction of the CYP1A1 gene. PMID: 23424205
41. CASP-9 polymorphisms are associated with susceptibility to low back pain during military training in Chinese soldiers. PMID: 23725396
42. Results found that polymorphisms of CASP9 and CASP10 genes may not contribute to CRC risk in Chinese population. PMID: 23303631
43. Proteolytic processing of the caspase-9 zymogen is required for apoptosome-mediated activation of caspase-9 PMID: 23572523
44. Polymorphism in Caspase 9 (-1263 A>G) was observed to be associated with susceptibility of papillary thyroid carcinoma (PTC). PMID: 22120515
45. Induction of caspase-9b expression is due to activation of hnRNP L via phosphorylation to compete/inhibit hnRNP U association with exon 3 of Casp9 mRNA. PMID: 23396972
46. These results clearly indicated that olive oil phenolic extract and gallic acid were able to inhibit the caspase 9 dependent apoptosis pathway in HeLa cells. PMID: 22086301
47. The overexpression of c-Jun, p73 and Casp-9 in thymic epithelial tumors is closely related with the pathogenesis and biological behavior of the neoplasms. PMID: 22974165
48. Data suggest that CD95L-triggered endogenous ceramide increase in Jurkat leukemia T cells is likely involved in the pro-apoptotic mitochondrial pathway leading to caspase-9 activation. PMID: 22306364
49. caspase-9 is the most important regulator in DLD-1, HCT-116, and HeLa cells PMID: 23038270
50. CASP9 promoter polymorphisms rs4645978 and rs4645981 are associated with breast cancer susceptibility; CASP9 transcriptional regulation is an important factor during breast cancer development PMID: 22981751
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蛋白家族：

Peptidase C14A family
組織特異性：

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
數據庫鏈接：

HGNC: 1511

OMIM: 602234

KEGG: hsa:842

STRING: 9606.ENSP00000330237

UniGene: Hs.329502

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