C5AR1 Recombinant Monoclonal Antibody

說明書

中文名稱：

C5AR1重組抗體
貨號：

CSB-RA003996A0HU
規格：

￥1320
圖片：
The Binding Activity of Human C5AR1-VLPs with Anti-C5AR1 recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human C5AR1 (CSB-MP003996HU) at 10 μg/mL can bind Anti-C5AR1 recombinant antibody, the EC₅₀ is 1.239-3.760 ng/mL.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA003996A0HU是針對C5aR1（C5a anaphylatoxin chemotactic receptor 1）的重組單克隆抗體，經ELISA嚴格驗證，適用于特異性檢測人源C5aR1蛋白。該抗體靶向補體系統關鍵受體C5aR1，該受體通過介導C5a趨化信號參與先天免疫應答、炎癥調控及細胞遷移過程，在感染性疾病、炎癥反應及免疫相關病理機制研究中具有重要價值。本產品采用重組表達技術制備，具有高批次穩定性和抗原結合一致性，ELISA驗證數據顯示其對天然及重組C5aR1蛋白均表現出優異檢測性能。適用于體外實驗中的靶蛋白定量檢測、信號通路研究以及炎癥相關疾病模型的機制探索，可支持免疫學研究中的受體功能分析、藥物靶點驗證及生物標志物檢測等科研需求。該抗體不適用于活體實驗或功能性研究，建議配合陽性對照及優化實驗條件使用。
Uniprot No.：

P21730
基因名：

C5AR1
別名：

C5AR1; C5AR; C5R1; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD antigen CD88
反應種屬：

Human
免疫原：

Recombinant Human C5AR1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

hIgG1
純化方式：

Affinity-chromatography
克隆號：

3A7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA
Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.
基因功能參考文獻：
1. the role of the active fragment complement C5a receptor (C5aR) in dental nerve regeneration in regards to local secretion of nerve growth factor (NGF) upon carious injury. PMID: 27539194
2. X ray structure of a thermostabilized C5aR1 (known as C5aR1 StaR) in complex with NDT9513727 PMID: 29300009
3. this study shows that C5a receptor (CD88) promotes motility and invasiveness of gastric cancer by activating RhoA PMID: 27756879
4. Serum containing active complement enhanced pneumococcal induced proinflammatory cytokine production through C5a release and C5aR crosstalk. PMID: 27721145
5. C5aR plays a role in T helper cell polarization induced by Mycobacterium tuberculosis and this effect is strain- and donor-dependent. PMID: 28237029
6. Panton-Valentine leucotoxin LukS-PV/LukF-PV and the gamma-hemolysin HlgC/HlgB, bind the C5a complement-derived peptide receptor, inducing intracellular calcium release in neutrophils. PMID: 28117704
7. TLR4 and C5aR crosstalk in dendritic cells induces a core regulatory network of RSK2, PI3Kbeta, SGK1, and FOXO transcription factors. PMID: 28733463
8. Results showed that 7-oxygenated cholesterol derivatives have differential effects on monocyte/macrophage expression of IL-8 and C5a receptor and that C5a receptor is involved in 7alphaOHChol-induced IL-8 expression via PI3K and MEK. Study demonstrated expression of IL-8 and C5a receptor primarily by 7alpha-hydroxycholesterol in monocytes/macrophages. PMID: 28323848
9. Reducing RPS19 in tumor cells or blocking the C5a receptor 1-RPS19 interaction decreases RPS19-mediated immunosuppression, impairs tumor growth, and delays the development of tumors in a transgenic model of breast cancer PMID: 28228558
10. C5aR expression in gastric cancer was associated with cancer progression, liver metastasis, and poor prognosis. PMID: 27688239
11. this study shows that downregulation of CD88 after stimulation with IL-8 is more pronounced in adults than in neonates, whereas fMLP induces changes in receptor expression that are of the same magnitude in neutrophils from neonates as from adults PMID: 27606963
12. Relation of C5aR expression to tumor development and poor prognosis of the breast cancer patients PMID: 26494574
13. The objective of the present study was to validate a receptor occupancy (RO) assay for a human anti-C5aR monoclonal antibody drug candidate, NNC0215-0384 (NN0384) PMID: 26084468
14. These data thus suggest that C5aR1 acts as an enhancer of CCR5-mediated HIV entry into macrophages, the targeting of which may prove useful to reduce HIV infection by R5 strains. PMID: 26537334
15. C5AR and C5L2-mediated neutrophil dysfunction is associated with a poor outcome in sepsis. PMID: 25726869
16. that C5aR stimulates cell invasion and migration via ERK1/2-mediated epithelial-mesenchymal transition in hepatocellular carcinoma cells PMID: 26475622
17. Inhibition of C5aR1 leads to a significant reduction of CD66b and CD11b expression indicating a lower neutrophil activation status and block of granule exocytosis. PMID: 26176669
18. that rs10853784 of C5aR1 gene are associated with coronary artery disease in Han population of China PMID: 25924896
19. 96.7% of the metastatic RCCs had C5aR expression, vs. 50.5% of the non-metastatic ones. C5a triggered ERK- and PI3Kdependent invasion of the C5aR-expressing renal carcinoma cells. The C5a-C5aR axis plays a crucial role in renal carcinoma cell invasion. PMID: 25682807
20. These results illustrate the novel activity of the C5a-C5aR axis that promotes human NPC cell proliferation through PCAFmediated STAT3 acetylation. PMID: 25174320
21. C5a/C5aR1 signalling plays a key role in disrupting blood-brain barrier integrity through different cascades including NF-KappaB translocation leading to altered tight junction proteins, Claudin-5 and ZO-1, and actin reorganization in the lupus setting PMID: 26059553
22. ). Low frequency of the C5aR 450 CT genotype, which apparently is a feature of certain kidney diseases, appears to be associated with good graft outcome in kidney transplantation. PMID: 25582053
23. C5AR1 SNP -1330T/G may serve as a useful pharmacodynamic predictor of nonsedating H1-antihistamine efficacy in chronic spontaneous urticaria (CSU), and -1330T alleles may be taken as a risk factor for CSU. PMID: 25455139
24. Porphyromonas gingivalis induces C5aR-TLR2 coassociation in neutrophils. PMID: 24922578
25. inhibition of TAFIa activity, however, was substrate-specific, because neither mAb inhibited the cleavage of thrombin-activated osteopontin and C5a by TAFIa, thus sparing the anti-inflammatory activity of TAFIa PMID: 24134522
26. C5aR and C5L2 may have roles in adiposity in women PMID: 24523571
27. The uPAR-C5aR axis, via the underlying NFkappaB transcriptional program, controls osteogenic differentiation with functional impact on vascular calcification in vivo. PMID: 24192237
28. High levels of CD88 are associated with poor prognosis of NSCLC. PMID: 23706417
29. C5aR cleavage is induced by neutrophil serine protease. The consequent impairment of C5aR activity contributes to suboptimal local neutrophil priming and bacterial clearance. PMID: 24446515
30. complement can regulate Muller cells through C5aR, which may contribute to the pathogenesis of retinal diseases, including DR. PMID: 24265019
31. C5aR is a regulatory switch that modulates TLR4 signaling via the Galphai/c-Raf/MEK/ERK signaling axis in human macrophages but not monocytes. PMID: 24043889
32. The C5aR binding Panton-Valentine Leukocidin component, LukS-PV, is a potent inhibitor of C5a-induced immune cell activation. PMID: 23684309
33. Data indicate that C5aR was aberrantly expressed in various cancers. PMID: 23287562
34. CD88 receptor expression is increased in brain from Alzheimer's disease patients compared to controls or vascular dementia patients. PMID: 23394121
35. C5aR-C5L2 homodimerization versus heterodimerization contribute to differential responses obtained following ASP vs C5a stimulation. PMID: 23268185
36. An RP S19 oligomer inhibitor and a C5aR inhibitor blocked the association of the K562 basophilic EB-like cells. PMID: 23346183
37. C5aR is shed during sepsis, resulting in the appearance of a previously undetected circulating C5aR in the serum of patients that negatively correlates with survival. PMID: 23479227
38. the neutrophil C5aR acts as a dual pro-inflammatory and pro-apoptosis receptor during the initiation and the resolution phases of acute inflammation, respectively--{review} PMID: 23402022
39. C5a receptor-dependent cell activation by physiological concentrations of desarginated C5a PMID: 23041570
40. C5aR was expressed in the thick ascending limb of Henle's loop and first part of the distal convoluted tubule. Under inflammatory conditions, C5aR was de novo expressed in proximal tubuli. PMID: 22960554
41. Complement C5a upregulates prolabor mediators in human gestational tissues via CD88-mediated NFKB activation. PMID: 22441801
42. Analysis of the 3'UTR of the C5aR mRNA showed that it contained two AU-rich elements (AREs), however site directed mutagenesis showed that these had no effect on basal expression. PMID: 22608366
43. The aim of this study was to investigate the genetic alterations and mRNA expression pattern of C5aR and C5L2 genes in neutrophils from attack-free familial Mediterranean fever patients. PMID: 22187344
44. the monocyte C5aR selectively activates the classical pathway with the binding of C5a and the alternative pathway with the binding of C5a/RP S19. PMID: 21613290
45. C5a, a complement activation product, is a useful marker in predicting the severity of necrotizing enterocolitis. PMID: 21628869
46. Among a cohort of 60 critically ill patients, C5a-mediated neutrophil dysfunction (as determined by reduced CD88 expression) was a strong predictor for subsequent acquisition of nosocomial infection PMID: 21292772
47. Data show that when a Gi/PI3K pathway is partially blocked, C5a receptors stimulate an alternative p38MAPK pathway. PMID: 20473571
48. expression profiles of CRegs and CD88 on leukocytes are specifically altered after polytrauma in humans, indicating a trauma-induced "complementopathy PMID: 19864971
49. The elevated local and systemic C5a levels, and additional individual local C5a increase during the exacerbation support the importance of C5a in Chronic Obstructive Pulmonary Disease. PMID: 20500690
50. Data show that C5L2 is predominantly intracellular, while C5aR is expressed on the plasma membrane, and that internalized C5aR following ligand binding is co-localized with both C5L2 and beta-arrestin. PMID: 20044484
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle.
蛋白家族：

G-protein coupled receptor 1 family
數據庫鏈接：

HGNC: 1338

OMIM: 113995

KEGG: hsa:728

STRING: 9606.ENSP00000347197

UniGene: Hs.2161

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