ANXA2 Recombinant Monoclonal Antibody

說明書

中文名稱：

ANXA2重組抗體
貨號：

CSB-RA987782A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: HEK293 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate
All lanes: ANXA2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 41 kDa
Observed band size: 36-45 kDa

IHC image of CSB-RA987782A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA987782A0HU ANXA2重組單克隆抗體是針對膜聯蛋白A2（ANXA2）靶點開發的高特異性科研試劑。該抗體通過重組表達技術制備，具有嚴格的批次間一致性，能夠精準識別ANXA2蛋白的天然構象。實驗驗證顯示其在Western Blot中可檢測到目標蛋白的清晰條帶，免疫組化染色呈現準確的組織定位，ELISA檢測靈敏度滿足常規實驗需求。推薦使用稀釋度為WB 1:500-1:2000，IHC 1:50-1:200。ANXA2作為鈣離子依賴性磷脂結合蛋白，參與細胞膜運輸、細胞骨架重構及血管生成調控，其異常表達與腫瘤轉移、血栓形成等病理過程密切相關。本產品適用于腫瘤微環境研究、細胞遷移機制分析、血管生物學實驗等基礎科研領域，特別推薦用于體外細胞模型研究、蛋白互作分析及病理組織樣本檢測。通過優化抗原表位設計，該抗體有效避免了與其他膜聯蛋白家族成員的交叉反應，為研究ANXA2在腫瘤侵襲轉移中的作用機制提供了可靠工具。
Uniprot No.：

P07355
基因名：

ANXA2
別名：

Annexin A2 (Annexin II) (Annexin-2) (Calpactin I heavy chain) (Calpactin-1 heavy chain) (Chromobindin-8) (Lipocortin II) (Placental anticoagulant protein IV) (PAP-IV) (Protein I) (p36), ANXA2, ANX2 ANX2L4 CAL1H LPC2D
反應種屬：

Human
免疫原：

A synthesized peptide derived from human ANXA2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

10H9
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.
基因功能參考文獻：
1. Mechanistic studies revealed that FOXD2AS1 upregulated the expression of the miR206 target gene ANXA2 in hepatocellular carcinoma by acting as a miR206 sponge. PMID: 30272362
2. study has localised AnxA2/S100A10 complexes to key anatomical locations in the placenta and suggests a role for this complex in amniotic epithelium, trophoblasts and syncytium, in addition to its well-known roles in endothelial cells PMID: 30143909
3. Inhibition of ANXA2 will affect the expression of downstream targets and downstream signaling pathways, leading to the suppression of the malignant progression of tumors. PMID: 30355917
4. our data suggest that overexpression of peroxiredoxin-2, annexin A2, and heat shock protein beta-1 was correlated with tumor invasion, metastasis, and poor prognosis, and therefore, these proteins may serve as potential diagnostic and therapeutic biomarkers. PMID: 29332450
5. aptamer wh6 can block MM cell adhesion to ANXA2 and block the proliferation of MM cells induced by ANXA2 PMID: 29906496
6. The results demonstrated that ANX2 and stromal tenascin C regulated invasion in addition to stemness and anoikis resistance, which are crucial for metastasis in the progression of pancreatic ductal adenocarcinoma. PMID: 29749431
7. highly expressed in gastric cancer, related to tumor size, histological differentiation, lymph node metastasis PMID: 29097873
8. models for annexin A2-mediated vesicle aggregation mechanisms. PMID: 29567212
9. Our profiling data provided a rich source for further study of the molecular mechanisms of acquired drug resistance inneuroblastoma ( NB). Further study may determine the role of ANXA2 as a prognostic biomarker and a potential therapeutic target for patients with multidrug-resistant NB. PMID: 28814318
10. These results suggested that increased ANXA2 and less expressed beta-catenin were correlated to adenomyosis-associated dysmenorrhea PMID: 28547742
11. These data suggested that Annexin A2 induces cisplatin resistance of non-small cell lung cancer (NSCLC)via regulation of JNK/c-Jun/p53 signaling, and provided an evidence that blockade of Annexin A2 could serve as a novel therapeutic approach for overcoming drug resistance in NSCLCs PMID: 28886730
12. Results show that Anxa2-Tyr23 phosphorylation is necessary for proliferation, invasion, and metastasis of breast cancer cells in vitro and in vivo. Tyr23-phosphorylated Anxa2 binds to STAT3 and serves as a key regulator of STAT3 signaling. PMID: 28470457
13. annexin A2 can potentially predict the development of pre-eclampsia. The low annexin A2 levels (<0.89 ng/ml) together with higher blood pressure and proteinuria constitute a higher risk to develop preeclampsia. PMID: 28501283
14. Variants in the Annexin A2 (ANXA2) gene directly influence plasma LDL-C levels. PMID: 28456096
15. this study showed that Annexin A2 inhibition suppresses proliferation and invasion in ovarian cancer via beta-catenin/EMT, proposing the potential role of Annexin A2 in the prevention and treatment of ovarian cancer PMID: 28440436
16. FNDC3B and ANXA2 expression correlate negatively with patient survival in hepatocellular carcinoma PMID: 27385217
17. UBAP2 formed a complex with Annexin A2 and promoted the degradation of Annexin A2 protein by ubiquitination, and then inhibited HCC progression. PMID: 27121050
18. results indicate that ANXA2 plays an important role to enhance the malignant behaviors of Hepatocellular carcinoma cells, and the enhancement is closely based on its remodeling to cell structures PMID: 27060670
19. Thus, tight regulation of AnxA2 function is an integral aspect of cellular homeostasis. The presence of AnxA2 in cancer cell-derived exosomes, as well as the potential regulation of exosomal AnxA2 by phosphorylation or other PTMs, are topics of great interes PMID: 28867585
20. Restoration of miR-101 expression reversed chemoresistance of drug-resistant gastric cancer cells by inhibiting viability and enhancing apoptosis via targeting ANXA2. PMID: 28609840
21. Annexin 2A (ANXA2) is over-expressed in Glioblastoma (GBM) and positively correlates with patient outcome. PMID: 27429043
22. Results confirm that both circRNA expression and even ANXA2 expression could be used as biomarkers of RR-MS with good values of specificity and sensitivity. PMID: 28651352
23. This study demonstrated that the expression level of AnxA2 was significantly higher in recurrent adamantinomatous craniopharyngioma compared with primary ones. AND AnxA2(+) adamantinomatous craniopharyngioma cells exhibited enhanced proliferation and migration ability compared with AnxA2(-) adamantinomatous craniopharyngioma cells. PMID: 27640198
24. Cercvical cancer stem cells express annexin II, an HPV co-receptor. PMID: 27008711
25. Annexin A2 (ANXA2) and prostate-specific antigen (PSA) proteins measured from biopsy tumour regions are unlikely to be good biomarkers for prediction of the clinical outcome of prostate cancer presenting with apparently localized disease. PMID: 29187477
26. anti-ANXA2 antibodies play a role in thrombotic mechanisms leading to recurrent pregnancy loss and placental vascular disease PMID: 27631133
27. results suggest that mycoplasma induces a resistance to multiple drugs in hepatocarcinoma cells which required the interaction of P37 and Annexin A2. The pathway downstream this interaction needs to be explored PMID: 28976984
28. Findings indicate that Munc13-4 supports acute WPB exocytosis by tethering WPBs to the plasma membrane via AnxA2-S100A10. PMID: 28450451
29. Detecting ANXA2 and ANXA4 expression may aid the evaluation of cervical carcinoma prognosis. PMID: 27402115
30. our studies show that this G-motif represents a novel and essential determinant for axonal localization of the Anxa2 mRNA mediated by the SMN complex. PMID: 28258160
31. The data data suggest that interactions between HIV-1 gp120 and A2 exist, though this interaction may be indirect. PMID: 27863502
32. Exosome-associated Annexin II plays an important role in angiogenesis and breast cancer metastasis. PMID: 27760843
33. ANXA2 stromal expression might play a key role in aggressive tumor phenotype associated with increased EMT CTCs release, however, other factors beyond ANXA2 are responsible for coagulation activation mediated by CTCs in breast cancer patients. PMID: 28476852
34. annexin A2 contributes to lung injury and fibrotic disease by mediating the fibrogenic actions of FXa. PMID: 28283478
35. Annexin A2 and HB-EGF are overexpressed and are being secreted into serum in Her-2 negative breast cancer patients. PMID: 27496793
36. results indicate that ANXA2 facilitates PS-ASO trafficking from early to late endosomes where it may also contribute to PS-ASO release PMID: 27378781
37. Annexins A2 and A8 in endothelial cell exocytosis and the control of vascular homeostasis PMID: 27451994
38. Rack1 regulated P-Glycoprotein activity, which was necessary for adriamycin-induced P-Glycoprotein - mediated phosphorylation of Anxa2 and Erk1/2. PMID: 27754360
39. The presence of vascular lesions in lupus nephritis (LN) appears to be associated with significant differences in the vascular expression of ANXA2. PMID: 26511441
40. expression elevated in lung squamous cell carcinoma tissue PMID: 28178129
41. annexin A2 but not follistatin is expressed in hepatocellular carcinoma PMID: 26189841
42. Our results place ANXA2 at the apex of a regulatory cascade that determines glioblastoma mesenchymal transformation and validate aSICS as a general methodology to uncover regulators of cancer subtypes. PMID: 27667176
43. Overexpression of ANXA2 is associated with colorectal cancer. PMID: 27468721
44. Annexin A2, up-regulated by IL-6, promotes ligament ossification in ankylosing spondylitis patients. PMID: 27697640
45. highly specific 30 kDa plant lectin could dissociate AnxA2 from endogenous lectin galectin-3 interaction at the cell surface. This dissociation could down-regulate Bcl-2 family proteins, cell proliferation, and migration simultaneously triggering cell apoptosis PMID: 26438086
46. Data show that ONECUT2, IGF2BP1, and ANXA2 proteins were confirmed to be microRNA-9 (miR-9) targets and aberrantly upregulated in hepatocellular carcinoma (HCC). PMID: 26547929
47. annexin A2 and S100A10 expressions are powerful predictors of serous ovarian cancer outcome. PMID: 26925708
48. expression of ANXA2 is associated with tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma PMID: 26196246
49. Anxa2 binds to STAT3 and promotes epithelial to mesenchymal transition in breast cancer. PMID: 26307676
50. the role of Axna2 is instructive for understanding the development of RA, suppress the effect of Axna2 might provide a new potential measure for treatment of RA. PMID: 26963384
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亞細胞定位：

Secreted, extracellular space, extracellular matrix, basement membrane. Melanosome. Note=In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
蛋白家族：

Annexin family
數據庫鏈接：

HGNC: 537

OMIM: 151740

KEGG: hsa:302

STRING: 9606.ENSP00000346032

UniGene: Hs.511605

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