ALYREF Recombinant Monoclonal Antibody
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中文名稱：ALYREF Recombinant Monoclonal Antibody
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貨號：CSB-RA146127A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: HEK293 whole cell lysate(20μg), Hela whole cell lysate(20μg),NTERA-2 whole cell lysate(20μg), A549 whole cell lysate(20μg), HL-60 whole cell lysate(20μg)
All lanes: THOC4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 27 kDa
Observed band size: 30 kDa
Exposure time：1min -
Overlay Peak curve showing HepG2 cells stained with CSB-RA146127A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：ALYREF
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別名：Ally of AML-1 and LEF-1 antibody; Ally of AML1 and LEF1 antibody; ALY antibody; ALY/REF antibody; Aly/REF export factor antibody; BEF antibody; bZIP enhancing factor antibody; bZIP-enhancing factor BEF antibody; REF antibody; THO complex 4 antibody; THO complex subunit 4 antibody; Tho4 antibody; thoc4 antibody; THOC4_HUMAN antibody; Transcriptional coactivator Aly/REF antibody; Transcriptional coactivator antibody
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反應種屬：Human
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免疫原：A synthesized peptide derived from human ALYREF
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：8F12
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability. Involved in mRNA export of C5-methylcytosine (m5C)-containing mRNAs: specifically recognizes and binds m5C mRNAs and mediates their nucleo-cytoplasmic shuttling.; Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation.
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基因功能參考文獻：
- Dysregulation of ALYREF-mediated mRNA export upon NSUN2 depletion could be restored by reconstitution of wild-type but not methyltransferase-defective NSUN2. PMID: 28418038
- ALYREF mainly binds to the 5' and the 3' regions of the mRNA. ALYREF is likely recruited to mRNAs by PABPN1 and the 3' processing machinery. PMID: 28934468
- The competition between hMTR4 and ALYREF determines exosome recruitment and functions in creating balanced nuclear RNA pools for degradation and export. PMID: 28801509
- small hairpin RNA-mediated down-regulation of TAP or Aly reduced nuclear export of HDAg-L and assembly of HDV virions. Furthermore, a peptide, TAT-HDAg-L(198-210), containing the 10-amino acid TAT peptide and HDAg-L(198-210), inhibited the interaction between HDAg-L and TAP and blocked HDV virion assembly and secretion. PMID: 27807029
- A short conserved motif in ALYREF directs cap- and exon junction complex-dependent assembly of export complexes on spliced mRNAs. PMID: 26773052
- the data provides the first site-specific description of how viral mRNA is locked by a herpes viral adaptor protein in complex with cellular ALYREF, giving herpesvirus access to the cellular mRNA export machinery PMID: 24550725
- a spatial map is produced in living cells of the sites for the interaction of two TREX subunits, Alyref and Chtop, with Nxf1. PMID: 23826332
- ALY is a novel E2F2-interacting protein and a relevant modulator of E2F-responsive gene expression. PMID: 23297349
- The interaction of the cellular export adaptor protein Aly/REF with ICP27 contributes to the efficiency of herpes simplex virus 1 mRNA export. PMID: 23637401
- High ALY expression is associated with lymph node metastasis in oral squamous cell carcinoma. PMID: 23242234
- Authors propose that Aly, THO and UAP56 form a highly integrated unit to associate with the spliced mRNA and function in mRNA export. PMID: 23222130
- REF/Aly-ORF57 interaction is not essential for KSHV lytic replication but may contribute to target RNA stability independent of effects on RNA export, suggesting a novel role for REF/Aly in viral RNA metabolism. PMID: 22761374
- When the TREX components UAP56 or Aly are knocked down, spliced mRNA, as well as total polyA+ RNA, accumulates in nuclear speckle domains. PMID: 20981025
- Results describe the subcellular localization of ICP27 and its colocalization with cellular RNA export factors Aly/REF and TAP/NXF1. PMID: 20015986
- suggest a central role for ALY in T-cell enhancer function and oncogene activation PMID: 17229714
- Aly is a physiological target of nuclear PI3K signaling, which regulates its subnuclear residency, cell proliferation, and mRNA export activities through nuclear Akt phosphorylation and phosphoinositide association. PMID: 18562279
- Adaptor Aly functions in the Tap-p15-mediated nuclear export of HSP70 mRNA. PMID: 19165146
- The authors demonstrate that ORF57 recruits several members of hTREX, namely Aly, UAP56 and hTHO-complex proteins, onto the viral mRNAs to assemble an export-competent ribonucleoprotein particle. PMID: 19264631
- TAP/NXF1, but not Aly/REF, is required for RNA export during HSV-1 infection. PMID: 19369354
- These results indicate that ICP27 RGG box methylation regulates interaction ALY and SRPK1 proteins. PMID: 19553338
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亞細胞定位：Nucleus. Nucleus speckle. Cytoplasm.
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蛋白家族：ALYREF family
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組織特異性：Expressed in a wide variety of cancer types.
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數據庫鏈接：
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