ADAM10 Recombinant Monoclonal Antibody

說(shuō)明書

中文名稱：

ADAM10重組抗體
貨號(hào)：

CSB-RA001273MA1HU
規(guī)格：

￥1320
圖片：
Overlay Peak curve showing Hela cells stained with CSB-RA001273MA1HU (red line) at 1:400. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA001273MA1HU ADAM10重組單克隆抗體是針對(duì)去整合素金屬蛋白酶10（ADAM10）靶點(diǎn)開發(fā)的高特異性科研試劑。該抗體經(jīng)ELISA和流式細(xì)胞術(shù)（FC）雙重驗(yàn)證，在FC應(yīng)用中推薦使用1:20-1:500的寬范圍稀釋梯度，展現(xiàn)優(yōu)異的檢測(cè)靈敏度和批次穩(wěn)定性。ADAM10作為調(diào)控Notch、E-cadherin等重要膜蛋白水解加工的關(guān)鍵蛋白酶，廣泛參與細(xì)胞黏附、信號(hào)轉(zhuǎn)導(dǎo)及病理生理過(guò)程。本產(chǎn)品適用于體外研究ADAM10在腫瘤微環(huán)境調(diào)控、神經(jīng)突觸可塑性以及炎癥反應(yīng)中的動(dòng)態(tài)表達(dá)特征，尤其適合通過(guò)流式細(xì)胞術(shù)追蹤活細(xì)胞表面ADAM10的實(shí)時(shí)表達(dá)變化。其重組單克隆特性保證了抗原表位識(shí)別的高度一致性，為神經(jīng)退行性疾病機(jī)制研究、免疫細(xì)胞功能分析及藥物靶點(diǎn)篩選提供可靠工具，滿足分子互作研究和功能驗(yàn)證實(shí)驗(yàn)中對(duì)靶蛋白精確定量檢測(cè)的需求。
Uniprot No.：

O14672
基因名：

ADAM10
別名：

A disintegrin and metalloprotease domain 10 antibody; A disintegrin and metalloproteinase domain 10 antibody; AD 10 antibody; AD10 antibody; AD18 antibody; ADA10_HUMAN antibody; ADAM 10 antibody; ADAM metallopeptidase domain 10 antibody; ADAM10 antibody; CD 156c antibody; CD156c antibody; CD156c antigen antibody; CDw156 antibody; disintegrin and metalloproteinase domain containing protein 10 antibody; Disintegrin and metalloproteinase domain-containing protein 10 antibody; HsT 18717 antibody; HsT18717 antibody; Kuz antibody; Kuzbanian antibody; Kuzbanian protein homolog antibody; Kuzbanian; Drosophila; homolog of antibody; MADM antibody; Mammalian disintegrin metalloprotease antibody; Mammalian disintegrin-metalloprotease antibody; RAK antibody
反應(yīng)種屬：

Human
免疫原：

Recombinant Human ADAM10 protein
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Mouse IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

6A8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, FC

推薦稀釋比：

Application	Recommended Dilution
FC	1:20-1:500

Protocols：

ELISA Protocol
Flow cytometry (FC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3. Mediates the proteolytic cleavage of IL6R and IL11RA, leading to the release of secreted forms of IL6R and IL11RA. Enhances the cleavage of CHL1 by BACE1. Cleaves NRCAM. Cleaves TREM2, resulting in shedding of the TREM2 ectodomain. Involved in the development and maturation of glomerular and coronary vasculature. During development of the cochlear organ of Corti, promotes pillar cell separation by forming a ternary complex with CADH1 and EPHA4 and cleaving CADH1 at adherens junctions. May regulate the EFNA5-EPHA3 signaling.; (Microbial infection) Promotes the cytotoxic activity of S.aureus hly by binding to the toxin at zonula adherens and promoting formation of toxin pores.
基因功能參考文獻(xiàn)：
1. SNHG20 could function as an oncogenic long non-coding RNA by regulating miR-140-5p-ADAM10 axis and MEK/ERK signaling pathway in cervical cancer. PMID: 29604594
2. restoration of ADAM10 expression partially reversed the effects of miR152 on cell proliferation and apoptosis in rheumatoid arthritisfibroblastlike synoviocytes. PMID: 29693139
3. Mechanisms underlying ADAM10 downregulation by miR-140-5p and suggests that dysfunctional regulation of ADAM10 expression is exacerbated by AD-related neurotoxic effects. PMID: 29253717
4. elevated expression of ADAM10 was associated with the pathogenesis and development of immune thrombocytopenia PMID: 29223855
5. Report overexpression of ADAM10 in oral squamous cell carcinomas, especially in OSCC with metastasis. PMID: 29895129
6. High ADAM10 expression is associated with meningococcal purpura fulminans. PMID: 29630665
7. The findings of the present study suggested that miR320a may function as a tumor suppressor in GC progression and potential therapeutic strategies for GC may be based on the miR320a/ADAM10 axis. PMID: 29152656
8. Insulin-like growth factor-1 activates different catalytic subunits p110 of PI3K in a cell-type-dependent manner to induce lipogenesis-dependent epithelial-mesenchymal transition through the regulation of ADAM10 and ADAM17. PMID: 28819788
9. mechanistic experiments revealed that ADAM10-RNAi resulted in an increase in E-cadherin and a decrease in N-cadherin and vimentin expression. Our study implies that high expression of ADAM10 promotes the proliferation and migration of hypopharyngeal squamous cell carcinoma (HSCC). These findings may help to provide a method for treatment of HSCC PMID: 28656294
10. Notch is ligand activated and undergoes DTX4-mediated ubiquitylation and bilateral endocytosis before ADAM10 processing PMID: 28611181
11. therapies against ADAM10 and ADAM17 may promote cancer stem cell migration away from the tumourigenic niche resulting in a differentiated phenotype that is more susceptible to treatment. PMID: 27541285
12. Presence of anti-ADAM10 auto-Antibodies seems to reflect the increased tumor expression of the immunogenic immature-ADAM10 in a group of Colorectal cancer patients, and is associated with a favourable prognosis in patients at stage III of the disease. PMID: 27517630
13. ADAM10 and ADAM17 are the best characterized members of the ADAM (A Disintegrin and Metalloproteinase) - family of transmembrane proteases. Both are involved diverse physiological and pathophysiological processes.For ADAM17 phosphatidylserine exposure is required to then induce its shedding function. PMID: 28624437
14. A better understanding of the regulatory mechanisms controlling the expression, subcellular localization and activity of ADAM10 will likely uncover suitable drug targets which will allow a more specific and fine-tuned modulation of its proteolytic activity PMID: 28624438
15. In the present study, the authors show that deletion of a triple serine (3S) motif (Ser-359 to Ser-361) adjacent to the cleavage site is sufficient to prevent IL-6R cleavage by ADAM17, but not ADAM10. We find that the impaired shedding is caused by the reduced distance between the cleavage site and the plasma membrane. PMID: 27151651
16. Here, I review some of the proposed functions of ADAM10 associated with intestinal crypt homeostasis and tumorigenesis within the gastrointestinal tract in vivo. PMID: 28739265
17. Study reports the structure of the ADAM10 ectodomain, providing fundamental insights into how substrate selectivity and regulation of catalytic activity is achieved in this important representative of the ADAM family of metalloproteases. PMID: 29224781
18. Data suggest that ADAM10 associates directly with all members of a subgroup of tetraspanins having eight cysteines in the large extracellular domain ('TspanC8'): Tspan5, Tspan10, Tspan14, Tspan15, Tspan17, and Tspan33. [REVIEW] PMID: 28687716
19. Results found ADAM10 expression under the regulation of MIR-655 which binds the 3'-UTR of ADAM10 mediating the progression of hepatocellular carcinoma. PMID: 27259866
20. Data suggest that activation of the metalloproteinase ADAM10 by signal peptide peptidase-like 3 (SPPL3) triggered by mutant BRAF(V600E) was a critical transformation event. PMID: 28292959
21. The ADAM17 messenger RNA (mRNA) and protein levels were significantly higher in the inferior turbinate than in nasal polyps (p < 0.05). The ADAM10 mRNA and protein levels did not differ significantly between NPs and inferior turbinates (p > 0.05). ADAM10 and ADAM17 were expressed primarily in inflammatory cells, submucosal glandular cells, and lining epithelial cells. PMID: 27012683
22. study confirms the importance of ICOSL shedding in ICOS/ICOSL function and expression and it identifies ADAM10 as the most important sheddase for controlling ICOSL levels PMID: 28814605
23. Inhibition of ADAM10 suppressed the expansion of NK cells and reduced the expression of CD16. PMID: 28982863
24. Platelet ADAM10 protein expression in patients with AD [Alzheimer's Disease] was positively influenced by serotoninergic medication PMID: 26555131
25. Tspan3 is a central endocytic membrane component regulating the expression of ADAM10, presenilin and the amyloid precursor protein. PMID: 27818272
26. Endothelial Tspan5- and Tspan17-ADAM10 complexes may regulate inflammation by maintaining normal VE-cadherin expression and promoting T lymphocyte transmigration. PMID: 28600292
27. Regulation of ADAM10 by the TspanC8 subgroup of tetraspanins, namely Tspan5, 10, 14, 15, 17 and 33 is reviewed. PMID: 28620033
28. active ADAM10 form marks cancer stem-like cells with active Notch signaling, known to mediate chemoresistance. PMID: 27503072
29. Data show that tetraspanin 33 (tspan33) is an early activation marker, and that disintegrin and metalloproteinase domain-containing protein 10 (ADAM10) protein expression does not correlate with Tspan33 expression in B cells. PMID: 28449222
30. High ADAM10 expression is associated with metastasis of hepatocellular carcinoma. PMID: 28184920
31. The dysregulation of ADAM10, Fas and FasL could be useful indicators of the progression and severity of OSCC. PMID: 27628319
32. 1,25D3 causes ectodomain shedding of TLR4 and thereby decreases the responsiveness of cells to LPS. ADAM10, activated by extracellular Ca2+ influx, was implicated in the ectodomain cleavage of TLR4. PMID: 28427048
33. The overexpression of MTERF4 induced a significant increase in the levels of APP protein and secreted Abeta 42 in HEK293-APPswe cells compared with control cells these results suggest that MTERF4 promotes the amyloidogenic processing of APP by inhibiting ADAM10 in HEK293-APPswe cells; therefore, MTERF4 may play an important role in the pathogenesis of Alzheimer's disease. PMID: 27894840
34. Pre-incubation with simvastatin prior to treatment with IL-1beta + Oncostatin M decreased the level of CD44 fragmentation, decreased the proportion of CD44 that transits into the lipid raft fractions, decreased ADAM10 activity and diminished the interaction between CD44 and ADAM10. PMID: 27242325
35. ADAM17 and ADAM10 cleave Nectin-4 and release soluble Nectin-4 (sN4). PMID: 28232483
36. Data show that mononuclear leukocytes (PBMC) AXL receptor tyrosine kinase (Axl) is rescued by combined matrix metalloproteases ADAM10 and TACE (ADAM17) inhibition. PMID: 27237127
37. we found that ADAM10 expression was associated with a more rapid metastatic progression confirming its role in uveal melanoma metastasis. PMID: 27546281
38. the TLR4/Gal-1 signaling pathway regulates lactate-mediated EMT processes through the activation of ADAM10 and ADAM17 in colon cancer cells. PMID: 27837433
39. Studies demonstrate a role for ADAM10 in the ectodomain shedding of LRP1 in the brain and the clearance of Abeta across the blood-brain barrier, which may provide a novel strategy for attenuating Abeta accumulation in the AD brain PMID: 27503326
40. In response to trastuzumab, both HER3 and the metalloprotease ADAM10 are simultaneously upregulated. The proteolytic activity of the latter then releases the HER3 ligand heregulin from the cell surface to activate HER3 and confer resistance to trastuzumab by inducing compensatory growth factor receptor signaling. PMID: 26863569
41. Active ADAM10 promotes the carcinogenesis, invasion, metastasis and proliferation of ESCC and controls invasion and metastasis at least in part through the shedding of E-cadherin activity. PMID: 26986985
42. We now show that ADAM10 is critical for alpha-hemolysin-mediated activation of the NLRP3 inflammasome in human monocytes as siRNA knockdown or chemical blockade of ADAM10-alpha-hemolysin interaction leads to diminished inflammasome activation and cell death by reducing the available ADAM10 on the cell surface. PMID: 27043625
43. ADAM10 was overexpressed in the posterior band of sections from some patients with temporomandibular joint disk disorders. PMID: 26947053
44. ADAM10 activity contributes to house dust mite-induced shedding of chemokines, including CCL20 PMID: 26296735
45. The results of this study demonistrated that age-dependent increase in ADAM10 levels and activity in platelets. PMID: 26757187
46. higher expression levels in the allergic nasal mucosa PMID: 26250527
47. The production of ADAM10-positive microvesicles from smoke-exposed neutrophils provides a novel molecular mechanism for the vastly accelerated risk of AAA in smokers. PMID: 26422658
48. activated microRNA-494-targets Bmi1 and ADAM10 by silibinin and ablates cancer stemness in head and neck squamous cell carcinomas PMID: 26090866
49. These findings indicate that different mechanisms regulate shear- and ligand-induced shedding and shear forces found within the vasculature can regulate ADAM10 activity. PMID: 26840909
50. Data suggest that large extracellular loop of Tspan14 mediates interaction with ADAM10, promotes ADAM10 maturation/trafficking to cell surface, and affects ADAM10 substrate specificity; ADAM10/Tspan14 interact in platelets/vascular endothelial cells. PMID: 26668317
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相關(guān)疾病：

Reticulate acropigmentation of Kitamura (RAK); Alzheimer disease 18 (AD18)
亞細(xì)胞定位：

Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle. Cell projection, axon. Cell projection, dendrite. Cell junction, adherens junction. Cytoplasm.
組織特異性：

Expressed in the brain (at protein level). Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 188

OMIM: 602192

KEGG: hsa:102

STRING: 9606.ENSP00000260408

UniGene: Hs.172028

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