ACTR1B Recombinant Monoclonal Antibody
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中文名稱：ACTR1B Recombinant Monoclonal Antibody
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貨號：CSB-RA115875A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: HEK293 whole cell lysate(20μg),MCF-7 whole cell lysate(20μg), PC-3 whole cell lysate(20μg), U-87MG whole cell lysate(20μg),U-251MG whole cell lysate(20μg),Jurkat whole cell lysate(20μg),Hela whole cell lysate(20μg), Mouse brain tissue lysate(20μg)
All lanes: ACTR1B antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time：2min -
IHC image of CSB-RA115875A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA115875A0HU diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of Hela cell with CSB-RA115875A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing Hela cells stained with CSB-RA115875A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：ACTR1B
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別名：Actin related protein 1B antibody; Actin-related protein 1B antibody; ACTR 1B antibody; ACTR1B antibody; ACTY_HUMAN antibody; ARP 1B antibody; ARP1 actin related protein 1 homolog B antibody; ARP1 actin related protein 1 homolog B centractin beta (yeast) antibody; ARP1 actin related protein 1 homolog B centractin beta antibody; ARP1 actin related protein 1 yeast homolog B centractin beta antibody; ARP1 yeast homolog B antibody; ARP1B antibody; Beta centractin antibody; Beta-centractin antibody; Centractin beta (Yeast) antibody; Centractin beta antibody; CTRN 2 antibody; CTRN2 antibody; PC 3 antibody; PC3 antibody
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反應種屬：Human, Mouse
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免疫原：A synthesized peptide derived from human ACTR1B
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：14D5
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.
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基因功能參考文獻：
- Data show that 4-oxo-4-HPR inhibited tubulin polymerization and modulated gene expression of spindle aberration associated genes Kif 1C, Kif 2A, Eg5, Tara, tankyrase-1, centractin, and TOGp. PMID: 19996280
- Down-regulation of beta-centractin is associated with hepatocellular carcinoma immune escape PMID: 17619203
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亞細胞定位：Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
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蛋白家族：Actin family, ARP1 subfamily
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數據庫鏈接：
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