ABCB1 Recombinant Monoclonal Antibody

說明書

中文名稱：

ABCB1重組抗體
貨號：

CSB-RA983422A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA983422A0HU diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of HepG2 cell with CSB-RA983422A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 583-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA983422A0HU重組單克隆抗體是針對ABCB1靶點開發(fā)的高特異性科研試劑。ABCB1基因編碼的P-糖蛋白作為ATP結(jié)合盒轉(zhuǎn)運蛋白家族成員，在多種腫瘤細胞中過表達并介導(dǎo)化療藥物外排，是導(dǎo)致腫瘤多藥耐藥的重要機制。該抗體經(jīng)嚴格質(zhì)控驗證，可精準識別天然構(gòu)象的ABCB1蛋白，在免疫組化（IHC）和免疫熒光（IF）實驗中推薦使用1:50-1:200的稀釋濃度，能清晰顯示靶蛋白在細胞膜上的定位特征，同時支持ELISA平臺進行定量檢測。實驗數(shù)據(jù)顯示其在人源組織樣本中具有優(yōu)異的組織特異性和低背景信號，適用于研究ABCB1在結(jié)腸、肝臟及血腦屏障等生理屏障中的藥物轉(zhuǎn)運功能，也可用于構(gòu)建腫瘤耐藥模型、分析抗癌藥物滲透效率及驗證外排泵抑制劑效果等基礎(chǔ)研究，為探索腫瘤耐藥機制及藥物開發(fā)提供可靠工具。
Uniprot No.：

P08183
基因名：

ABCB1
別名：

Multidrug resistance protein 1 (EC 3.6.3.44) (ATP-binding cassette sub-family B member 1) (P-glycoprotein 1) (CD antigen CD243), ABCB1, MDR1 PGY1
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human ABCB1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

11H10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
基因功能參考文獻：
1. Conformational dynamics of P-glycoprotein in lipid nanodiscs and detergent micelles reveal complex motions on a wide time scale. PMID: 29511086
2. Study demonstrated that TWIST protein expression was elevated in liver cancer tissue specimens and was positively correlated with MDR1 expression. Knockdown of TWIST increased the sensitivity of RHepG2 cells to antineoplastic agents through a reduction in MDR1 expression and drug efflux ability. PMID: 30066890
3. the over-expression of SLC7A11, or supplementation with sufficiently cystine, or treatment with N-acetylcysteine significantly decreased P-gp expression and activity. It was suggested that ROS and SLC7A11/cystine were the two relevant factors responsible for the expression and function of P-gp, and that SLC7A11 might be a potential target modulating ADR resistance. PMID: 28630426
4. Data provide a mechanistic explanation for the differential effects of ABCB1 haplotypes on its promoter activity and underscore the importance of evaluating genetic variants in the context of haplotypes rather than individual SNPs when investigating their effects on gene/protein expression and disease risk. PMID: 30277801
5. CYP3A5, ABCB1 and two POR genotypes were assessed by real-time PCR. PMID: 28094348
6. ABCB1 C3435T polymorphism can affect the elimination of some antipsychotic/antidepressant drugs. PMID: 29723928
7. High BCL11A and MDR1 expression was associated with a poor response to chemotherapy. PMID: 29469608
8. The methylation/expression ratios of ABCB1 were unaffected by increasing BMI values. PMID: 27897267
9. Neither the ABCB1 C3435T nor the SLCO1B1 T521C polymorphism affected edoxaban PK. PMID: 27897269
10. Our results show that ABCB1 C3435T polymorphism may modulate serum THC levels in chronic heavy cannabis users. The exact mechanisms and roles that this may play in cannabis dependence genesis and evolution remain to be elucidated. These results should be controlled in a replication study using a larger population. PMID: 28917442
11. MDR1 is not expressed on erythrocyte membrane. PMID: 29098941
12. The genetic polymorphisms of the multi-drug resistance-1 (MDR-1) and human cytochrome P450 3A (CYP3A4 and CYP3A5) genes were analyzed and compared between steroid sensitive, steroid resistant and control groups. PMID: 30143489
13. This meta-analysis suggests that the MDR1 C > T polymorphism was not associated with the risk of MM. To confirm these findings, further comprehensive and well-designed studies are needed. PMID: 29495954
14. High MDR1 expression is associated with chemoresistance in ovarian cancer. PMID: 29956807
15. 13-cis-retinoic acid, retinol and retinyl-acetate inhibited the Pgp and ABCG2 mediated substrate transport as well as the substrate stimulated ATPase activity of these transporters. PMID: 28145501
16. Haplotype analysis of ABCB1 conducted in patients with bullous pemphigoid demonstrated that the 1236T-2677G-3435T haplotype may protect against development of disease. PMID: 29948283
17. High ABCB1 expression is associated with Bendamustine-resistance in Mantle Cell Lymphoma. PMID: 29695404
18. Data show that multidrug resistance gene 1 (MDR1) expression was associated with worsen survival of esophageal squamous cell carcinoma (ESCC) patients with cisplatin-based chemotherapy. PMID: 29107111
19. ABCB1 variation affected myelosuppression, progression-free survival and overall survival in ovarian cancer patients who were treated with paclitaxel and carboplatin PMID: 29504705
20. Our data showed that the expression of the MDR1 gene was significantly higher in malignant tissue than in the normal tissues of patients with STS. In addition, high MDR1 expression was significantly associated with local advances, as well as poor response to treatment. PMID: 29689707
21. These results indicated that MG132 reversed the MDR of hypopharyngeal carcinoma by downregulating Pgp/Pgp, and the underlying mechanism may be associated with the activation the of the JNK signaling pathway PMID: 29901180
22. P-glycoprotein on peripheral blood leukocytes of systemic lupus erythematosus (SLE) patients with lupus arthritis may have a role in clinical response to methotrexate PMID: 28616661
23. This study elaborated a novel UCA1/miR-129/ABCB1 regulatory axis underlying paclitaxel resistance of ovarian cancer cells. PMID: 29777711
24. ABCB1 3435C>T and ATIC 347C>G SNPs were associated with abnormal hepatic enzyme elevation by methotrexate treatment in patients with rheumatoid arthritis. PMID: 29252093
25. The role of multidrug resistance 1 gene (MDR1 or ABCB1) polymorphism G2677T was studied in relation to paroxetine therapeutic efficacy and its side effects, as well as its association with selected demographic and clinical characteristics of patients with depressive disorder. PMID: 29754150
26. alterations in latrophilin expression occur in AML cells expressing P-gp PMID: 29938681
27. This study demonstrated that the The G2677T T and C3435T T alleles as well as the TT, CTT and TTT haplotypes seemed to be significantly associated with drug-resistance epilepsy in Tunisian epileptic population. PMID: 29198163
28. Pharmacokinetics of aripiprazole is affected by sex and ABCB1 gene phenotype PMID: 29325225
29. There were no significant differences in the bosutinib C0 between genotypes for ABCB1, ABCG2, and CYP3A4 polymorphisms. PMID: 29736778
30. Studies show a connection between UDP-glucose ceramide glucosyltransferase (UGCG) and multidrug resistance protein 1 (MDR1) overexpression and multidrug resistance development [Review]. PMID: 29409484
31. Due to the downregulation of MDR1 and MRP1, the intracellular accumulation of ADM was increased in the transfected cells compared with that in the parental K562/ADM cells..Our research revealed that protein expression of the ERK signaling pathway was inhibited by downregulating TRIB2, indicating that the ERK pathway was involved in cell drug resistance and proliferation. PMID: 29436678
32. Asiatic Acid strongly inhibited P-GP expression by blocking MDR1 gene transcription and increased the intracellular accumulation of the P-GP substrate Rhodamine 123 in MDR1-overexpressing cisplatin (DDP)-resistant lung cancer cells, A549/DDP. PMID: 29768255
33. Ceritinib enhanced the efficacy of substrate chemotherapeutic agent in ABCB1-overexpressing K562/adr leukemia cells both in vitro and in vivo models, but neither in sensitive parental K562 leukemia cells nor in ABCC1-overexpressing HL-60/adr leukemia cells. PMID: 29742496
34. Polymorphism of ABCB1 rs1045642-T variant allele is associated with colorectal cancer. PMID: 29282011
35. There is a certain correlation between the APC gene and ovarian tumors, and the APC gene mediates the apoptosis of tumor cells through the MDR-1/CLCX-1 signaling pathway. PMID: 29921377
36. ABCB1/Pgp expression, activity, and single nucleotide polymorphisms (SNPs) in chronic myeloid leukemia. [review] PMID: 29316665
37. High MDR1 expression is associated with primary myelodysplastic syndrome. PMID: 27605311
38. analysis of the structure of zosuquidar and UIC2-bound human-mouse chimeric ABCB1 PMID: 29440498
39. ABCB1 gene polymorphisms are not associated with response to chemotherapy in breast cancer. PMID: 29113667
40. Patients prescribed with short-term low-dose atorvastatin and carrying ABCB1 (rs1128503) or ABCG2 (rs2231142) SNPs did not show differences in LDL-C response (P>.05). PMID: 28833323
41. Genotypes of UGT1A and ABCB1 were significantly associated with changes in moxifloxacin pharmacokinetic parameters. PMID: 29210323
42. G2677T/A polymorphism of MDR1 may play a significant role in the development of drug-resistant epilepsy in the Polish patients PMID: 28608314
43. No association was found between the ABCB1 SNPs and Crohn's disease in the two populations PMID: 28759738
44. icaritin (ICT) decreased the mRNA and protein levels of multidrug resistance protein 1 (MDR1) in MG-63 doxorubicin-resistant (MG-63/DOX) cells. PMID: 29425587
45. We demonstrate for the first time that the effect of ABCB1 diplotype on tacrolimus disposition is dependent on both CYP3A5 and CYP3A4 genotype. PMID: 27378609
46. these results address key concerns in the field, including that of cross-resistance between taxanes and highlighting a mechanism of cabazitaxel resistance involving ABCB1 PMID: 28698198
47. CYP3A and ABCB1 SNPs were detected in 521 recipients. PMID: 28952408
48. MDR-1 C3435T gene polymorphism was associated with right ventricular dysfunction in patients with chronic obstructive pulmonary disease. PMID: 29791609
49. data suggested that hypomethylation agent decitabine restores drug sensitivity in the P-gp-induced MDR phenotype by depressing of P-gp activity as drug pump partly through MAPK signaling pathway. PMID: 28405849
50. None of the genotypes in ABCB1 1236 C>T, 2677 G>T/A, 3435 C>T, and 4036 A>G correlated with plasma dolutegravir concentration..The speculated peak level of plasma dolutegravir concentration was significantly higher in ABCG2 genetic variant holders, probably, at least in part, because of low expression levels of efflux transporters in the intestines associated with these genetic variants. PMID: 28858994
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相關(guān)疾病：

Inflammatory bowel disease 13 (IBD13)
亞細胞定位：

Cell membrane; Multi-pass membrane protein. Apical cell membrane.
蛋白家族：

ABC transporter superfamily, ABCB family, Multidrug resistance exporter (TC 3.A.1.201) subfamily
組織特異性：

Expressed in liver, kidney, small intestine and brain.
數(shù)據(jù)庫鏈接：

HGNC: 40

OMIM: 171050

KEGG: hsa:5243

STRING: 9606.ENSP00000265724

UniGene: Hs.489033

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