oprF Antibody

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文獻引用 (1) 說明書

中文名稱：

oprF兔多克隆抗體
貨號：

CSB-PA318417LA01EZX
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in Recombinant protein
All lanes: oprF antibody at 5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39.3 kDa
Observed band size: 44 kDa
其他：

產(chǎn)品詳情
產(chǎn)品評價
引用文獻
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

The OprF polyclonal antibody is a rabbit antibody that specifically recognizes Pseudomonas aeruginosa OprF protein. Its immunogen is the recombinant Pseudomonas aeruginosa OprF protein (25-350aa). It has been validated for use in ELISA and WB assays. The antibody has been purified using protein G and has a purity level of over 95%.

OprF is one of the major porins in the outer membrane of P. aeruginosa, participating in the uptake of nutrients and antibiotics, as well as in the efflux of toxins and metabolic waste products. It has also been implicated in virulence and pathogenesis in P. aeruginosa.
產(chǎn)品名稱：

Rabbit anti-Pseudomonas aeruginosa oprF Polyclonal antibody
Uniprot No.：

P13794
基因名：

oprF
別名：

oprF antibody; PA1777 antibody; Outer membrane porin F antibody
宿主：

Rabbit
反應(yīng)種屬：

Pseudomonas aeruginosa
免疫原：

Recombinant Pseudomonas aeruginosa Outer membrane porin F protein (25-350AA)
免疫原種屬：

Pseudomonas aeruginosa

標記方式：

Non-conjugated

本頁面中的產(chǎn)品，oprF Antibody (CSB-PA318417LA01EZX)，的標記方式是Non-conjugated。對于oprF Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA318417LB01EZX	oprF Antibody, HRP conjugated	ELISA
FITC	CSB-PA318417LC01EZX	oprF Antibody, FITC conjugated
Biotin	CSB-PA318417LD01EZX	oprF Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻

Biophysical and proteomic analyses of Pseudomonas syringae pv. tomato DC3000 extracellular vesicles suggest adaptive functions during plant infection M Janda,mBio,2023

產(chǎn)品評價

靶點詳情

功能：

Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.
基因功能參考文獻：
1. OprF (an outer membrane porin, highly conserved in the Pseudomonas) binds human C3b. PMID: 25964476
2. LecB binds to the outer membrane protein OprF PMID: 23056489
3. oprF transcription was increased in response to low NaCl or high sucrose concentrations, and this induced transcription was strongly impaired in the absence of SigX. PMID: 22685281
4. Prevention of disulfide bond formation in OprF increases its pore-forming activity. PMID: 20978537
5. This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production PMID: 21189321
6. Data show that the recombinant plasmid pIRES-tPA-OprF-MyD88 has been successfully constructed and tPA-OprF and MyD88 protein can be highly expressed in transfected cells. PMID: 19257978
7. findings suggest that OprF could adopt two alternative conformations in the outer membrane and that folding is thermoregulated PMID: 15528532
8. findings demonstrate that interferon-gamma binds to an outer membrane protein in Pseudomonas aeruginosa, OprF, resulting in the expression of a quorum-sensing dependent virulence determinant, the PA-I lectin PMID: 16051797
9. analysis of OprF from Pseudomonas aeruginosa PMID: 16397890
10. OprF exists in two different conformations PMID: 16595653
11. analysis of Pseudomonas aeruginosa porin OprF PMID: 16617058
12. Major outer membrane protein F (OprF) is identified as a protein with important functions; mutant Pseudomonas aeruginosa deficient in OprF negates the antibacterial role of host neutrophil elastase both in vitro and in a pneumonia model. PMID: 18802098
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亞細胞定位：

Cell outer membrane; Multi-pass membrane protein.
蛋白家族：

OmpA family
數(shù)據(jù)庫鏈接：

KEGG: pae:PA1777

STRING: 208964.PA1777

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