UBE2M Antibody

說(shuō)明書

中文名稱：

UBE2M兔多克隆抗體
貨號(hào)：

CSB-PA025468OA01HU
規(guī)格：

￥440
圖片：
IHC image of CSB-PA025468OA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA025468OA01HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) UBE2M Polyclonal antibody
Uniprot No.：

P61081
基因名：

UBE2M
別名：

hUbc12 antibody; NEDD8 carrier protein antibody; NEDD8 conjugating enzyme Ubc12 antibody; NEDD8 protein ligase antibody; NEDD8-conjugating enzyme Ubc12 antibody; UBC-RS2 antibody; UBC12 antibody; UBC12_HUMAN antibody; ube2m antibody; ubiquitin carrier protein M antibody; Ubiquitin conjugating enzyme E2 M antibody; Ubiquitin-conjugating enzyme E2 M antibody; ubiquitin-protein ligase M antibody; yeast UBC12 homolog antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Peptide sequence from Human NEDD8-conjugating enzyme Ubc12 protein (11-28AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，UBE2M Antibody (CSB-PA025468OA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于UBE2M Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA025468OB01HU	UBE2M Antibody, HRP conjugated	ELISA
FITC	CSB-PA025468OC01HU	UBE2M Antibody, FITC conjugated
Biotin	CSB-PA025468OD01HU	UBE2M Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
基因功能參考文獻(xiàn)：
1. the DCN1-UBC12 interaction is inhibited by DI-591, a high-affinity, cell-permeable small-molecule inhibitor that binds to purified recombinant human DCN1 and DCN2 proteins with K i values of 10-12 nM PMID: 29074978
2. Furthermore, the authors characterized SENP8/DEN1 as the protease that counteracts Ubc12 auto-neddylation, and observed aberrant neddylation of Ubc12 and other NEDD8 conjugation pathway components in SENP8-deficient cells. PMID: 28475037
3. GlyRS preferentially binds and protects Ubc12. PMID: 27348078
4. Polymorphisms in the UBE2M gene are associated with cetuximab efficacy in colorectal cancer. PMID: 26206335
5. UBE2M is required to maintain genome integrity by activating multiple Cullin ligases throughout the cell cycle. PMID: 25025768
6. Distinct preferences of UBC12 and UBE2F peptides for inhibiting different DCNLs, including the oncogenic DCNL1. PMID: 23201271
7. Our results demonstrate a role for UBE2M in mediating cytotoxicity of gemcitabine in human urothelial carcinoma cells. PMID: 21477582
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蛋白家族：

Ubiquitin-conjugating enzyme family, UBC12 subfamily
數(shù)據(jù)庫(kù)鏈接：

HGNC: 12491

OMIM: 603173

KEGG: hsa:9040

STRING: 9606.ENSP00000253023

UniGene: Hs.406068

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