TRIM8 Antibody

中文名稱：

TRIM8兔多克隆抗體
貨號：

CSB-PA871629LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA871629LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA871629LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) TRIM8 Polyclonal antibody
Uniprot No.：

Q9BZR9
基因名：

TRIM8
別名：

AA408830 antibody; BB006755 antibody; GERP antibody; Glioblastoma-expressed RING finger protein antibody; Lioblastoma expressed ring finger protein antibody; Probable E3 ubiquitin-protein ligase TRIM8 antibody; RING finger protein 27 antibody; RNF27 antibody; RP11-47A8.4 antibody; TRIM8 antibody; TRIM8_HUMAN antibody; Tripartite motif containing 8 antibody; Tripartite motif protein TRIM8 antibody; Tripartite motif-containing protein 8 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Probable E3 ubiquitin-protein ligase TRIM8 protein (367-517AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，TRIM8 Antibody (CSB-PA871629LA01HU)，的標記方式是Non-conjugated。對于TRIM8 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA871629LB01HU	TRIM8 Antibody, HRP conjugated	ELISA
FITC	CSB-PA871629LC01HU	TRIM8 Antibody, FITC conjugated
Biotin	CSB-PA871629LD01HU	TRIM8 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase that participates in multiple biological processes including cell survival, differentiation, apoptosis, and in particular, the innate immune response. Participates in the activation of interferon-gamma signaling by promoting proteasomal degradation of the repressor SOCS1. Plays a positive role in the TNFalpha and IL-1beta signaling pathways. Mechanistically, induces the 'Lys-63'-linked polyubiquitination of MAP3K7/TAK1 component leading to the activation of NF-kappa-B. Modulates also STAT3 activity through negative regulation of PIAS3, either by degradation of PIAS3 through the ubiquitin-proteasome pathway or exclusion of PIAS3 from the nucleus. Negatively regulates TLR3/4-mediated innate immune response by catalyzing 'Lys-6'- and 'Lys-33'-linked polyubiquitination of TICAM1 and thereby disrupting the TICAM1-TBK1 interaction.
基因功能參考文獻：
1. TRIM8 activates STAT3 by suppressing the expression of PIAS3, an inhibitor of STAT3, most likely through E3-mediated ubiquitination and proteasomal degradation. PMID: 28100038
2. we provided evidence that TRIM8 and its regulators miR-17-5p and miR-106b-5 participate to a feedback loop controlling cell proliferation through the reciprocal modulation of p53, miR-34a and N-MYC. PMID: 28327152
3. A pathogenic effect of a heterozygous truncating mutation in the tripartite motif containing 8 (TRIM8) gene on the postnatal development of the human brain. PMID: 27346735
4. Our study provides evidences that TRIM8 may participate in the carcinogenesis and progression of glioma and that the transcriptional repression of TRIM8 might have potential value for predicting poor prognosis in glioma patients. PMID: 26077989
5. These findings provide the first mechanistic link between TRIM8 and the drug resistance of clear cell Renal Cell Carcinoma PMID: 25277184
6. Nucleo-cytoplasmic trafficking of TRIM8, a novel oncogene, is involved in positive regulation of TNF induced NF-kappaB pathway. PMID: 23152791
7. TRIM8 is a p53 direct target gene that induces p53 stabilization and promotes the degradation of MDM2 protein. PMID: 22262183
8. Tripartite motif 8 (TRIM8) modulates TNFalpha- and IL-1beta-triggered NF-kappaB activation by targeting TAK1 for K63-linked polyubiquitination. PMID: 22084099
9. TRIM8 modulates translocation of phosphorylated STAT3 into the nucleus through interaction with Hsp90beta and consequently regulates transcription of Nanog in embryonic stem cells. PMID: 21689689
10. These findings indicate that TRIM8 enhances the STAT3-dependent signal pathway by inhibiting the function of PIAS3. PMID: 20516148
11. interacts with SOCS-1 PMID: 12163497
12. Gerp transcribes interferon-gamma in epithelial and lymphoid cells and is expressed almost ubiquitously in tissues. PMID: 15345195
13. Among genes correlated to nodal metastatic progression, we verified in vitro that NM23-H3 reduced cell motility and TRIM8 were a growth suppressor. PMID: 17900511
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亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

TRIM/RBCC family
組織特異性：

Widely expressed.
數據庫鏈接：

HGNC: 15579

OMIM: 606125

KEGG: hsa:81603

STRING: 9606.ENSP00000302120

UniGene: Hs.336810

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