STIM1 Antibody, Biotin conjugated

說明書

中文名稱：

STIM1兔多克隆抗體, Biotin偶聯
貨號：

CSB-PA022829LD01HU
規格：

￥880
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) STIM1 Polyclonal antibody
Uniprot No.：

Q13586
基因名：

STIM1
別名：

D11S4896E antibody; GOK antibody; OTTHUMP00000164512 antibody; OTTHUMP00000229140 antibody; OTTHUMP00000230742 antibody; SIM antibody; STIM 1 antibody; STIM1 antibody; Stim1 stromal interaction molecule 1 antibody; STIM1_HUMAN antibody; STIM1L antibody; Stromal interaction molecule 1 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Stromal interaction molecule 1 protein (2-350AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Biotin
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA
Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit ORAI1. Involved in enamel formation. Activated following interaction with STIMATE, leading to promote STIM1 conformational switch.
基因功能參考文獻：
1. these data suggest that the loss of STIM1 and impaired store-operated calcium entry contribute to ER Ca(2+) dyshomeostasis under diabetic conditions PMID: 30131394
2. deletion of the oncogenic KRAS allele resulted in enhanced STIM1 expression and greater Ca(2+) influx. PMID: 29748135
3. Pore helix rotation by stromal interaction molecule 1 (STIM1) indicates the dynamic coupling between CRAC channel Orai1 gating and ion selectivity. PMID: 28220789
4. the conserved portion of the Orai N terminus is essential for STIM1, as it fine-tunes the open Orai channel gating, thereby establishing authentic CRAC channel activity. PMID: 29237734
5. The constitutive activation of Orai1 in Stormorken syndrome mainly involves the CRAC-activating domain CAD/SOAR of STIM1, the exposure of which is regulated by the molecular interplay between three cytosolic STIM1 coiled-coil (CC) domains. PMID: 29483506
6. Each STIM1 protein monomer within the dimer interacts independently with single Orai1 subunits to mediate cross-linking between Orai1 channels. PMID: 29581306
7. Results show that phosphorylation of STIM1 at Y361 is required to trigger store-operated Ca(2+) entry, and for STIM1-Orai1 interaction and recruitment of Orai1 to STIM1 puncta. Also, STIM1 phosphorylation at Y361 residue is required for increasing vascular permeability. PMID: 28218251
8. The phosphorylation of the T389 residue in STIM1 is an essential feature determining the activity of store-independent ARC channels, and is mediated by an AKAP79/PKA-dependent process. PMID: 27847114
9. An Orai1 C-terminal STIM1-binding site, situated far from the N-terminal pore helix, alone provides the trigger that is necessary and sufficient for channel gating. PMID: 27929067
10. Stim1 silencing also reduced cell viability, caused cell apoptosis and cell cycle arrest in Multiple myeloma cell lines. PMID: 30114708
11. Study shows that STIM1 and ORAI1 promotes membrane ruffling by showing that phospho-STIM1 localizes at the leading edge of cells, and that both phospho-STIM1 and ORAI1 co-localize with cortactin. PMID: 28341841
12. Results show that STIM1 is overexpressed in prostate cancer tissues and, reveal that it may act as a novel regulator of migration and invasion of prostate cancer cells through the activation of the PI3K/Akt signaling pathway. PMID: 29048678
13. In patients with ORAI1 and STIM1 loss of function mutations, effector functions were preserved in polymorphonuclear neutrophils. PMID: 26670474
14. This activation was sensitive to Src kinase inhibition, but not to CAMKII nor PKC inhibition, a result that sets STIM1 and SOCE as downstream targets of the axis Src-Raf-MEK-ERK, rather than upstream regulators. PMID: 28866365
15. STIM1 plays an important role in paraquat induced cell cycle arrest and cell death in acute lung injury. PMID: 29709426
16. we demonstrated that STIM1 could be involved in breast tumorigenesis and progression PMID: 28869106
17. Study provide clear evidence that STIM1 has a cholesterol-binding domain located inside the SOAR region and modulates Orai1 channels. A functional association of STIM1 and SOAR to cholesterol was shown, indicating a close proximity of SOAR to the inner layer of the plasma membrane. In contrast, the depletion of cholesterol induces the SOAR detachment from the plasma membrane and enhances its association to Orai1. PMID: 27459950
18. Our study clarifies the roles of major STIM1 functional domains in maintaining a quiescent configuration of STIM1 to prevent preactivation of SOCE. PMID: 28231751
19. The results of this study concluded that the phenotype associated with activating STIM1 mutations frequently includes extra-neuromuscular features such as hypocalcaemia, hypo-/asplenia and platelet dysfunction regardless of mutation domain. PMID: 28624464
20. Ca(2+) unbinding in the STIM1 luminal domains initiates the conformational change allowing SOAR domain liberation and clustering, leading to Orai1 channel activation. PMID: 28724757
21. calmodulin (CaM) associates with the core region of STIM1 in a Ca2+-dependent manner. Consequently, calcium-bound CaM disrupts the STIM1-Orai1 complex and disassembles STIM1 oligomers, thereby inducing deactivation of the store-operated calcium entry. PMID: 29051492
22. STIM1 knockdown may inhibit the migration and invasion of A549 cells in vitro, and metastasis in vivo. PMID: 28713917
23. STIM1 is significantly upregulated in GC and that STIM1 overexpression is associated with a poor prognosis in GC patients with LNM and an advanced TNM stage. Therefore, STIM1 may be a useful prognostic marker for GC. PMID: 28534934
24. Here, we summarize the recent discoveries on the structure-function relationship of Orai1, as well as its interaction with the native channel opener STIM1 and chemical modulator 2-aminoethoxydiphenyl borate PMID: 27753099
25. the elevated expression of STIM1 might be involved in lung tumorigenesis. PMID: 27863410
26. TRPC1-STIM1 activation modulates transforming growth factor beta-induced epithelial-to-mesenchymal transition and cell migration. PMID: 27793015
27. Data show that the G98S and V107M mutations generate constitutively permeable ORAI1 channels, whereas T184M alters the channel permeability only in the presence of STIM1. PMID: 28058752
28. STIM1 contributes to the development of OTSCC. PMID: 28108672
29. Our findings demonstrate for the first time that STIM1 and store-operated calcium entry signaling are involved in the TGF-beta-induced suppression of cell proliferation PMID: 26919241
30. Cultured hippocampal neurons expressing mutant PS1 had attenuated CCE that was associated with destabilized dendritic spines, which were rescued by either gamma-secretase inhibition or overexpression of STIM1. PMID: 27601731
31. These findings suggest that the surface location of SARAF is dependent on the expression of STIM1 in the plasma membrane. PMID: 27414851
32. sp(2) -Iminosugar alpha-glucosidase inhibitor 1-C-octyl-2-oxa-3-oxocastanospermine inhibits breast cancer cell migration via beta1-integrin, Stim1, and FAK signaling pathways. PMID: 28145580
33. The STIM1 protein play important roles in TGF-beta-induced EMT and these effects are related to both store-operated calcium entry and non-store-operated calcium entry. PMID: 28479254
34. STIM1-dependent control of Ca2+ clearance regulates NFAT activity during T-cell activation PMID: 27528628
35. RASSF4 controls store operated calcium entry and endoplasmic reticulum-plasma membrane junctions through ARF6-dependent regulation of PM PI(4,5)P2 levels, pivotal for a variety of physiological processes. PMID: 28600435
36. conformational changes in STIM1 upon calcium release PMID: 28151650
37. STIM1-induced Ca(2+) signaling activates Pyk2 to inhibit the interaction of VE-PTP and VE-cadherin and hence increase endothelial permeability. PMID: 28385807
38. Elevated Orai1 and STIM1 expressions upregulate MACC1 expression to promote tumor cell proliferation, metabolism, migration, and invasion in human gastric cancer. PMID: 27431311
39. It represents as a target of cisplatin and blockade of Stim1-mediated Ca(2+) entry may be a useful strategy to improve the efficacy of cisplatin to treat osteosarcoma. PMID: 28326487
40. STIM1L and TRPC1/4 are working together in myotubes to ensure efficient store refilling and a proper differentiation program. PMID: 28185894
41. SARAF modulates TRPC1, but not TRPC6, channel function in a STIM1-independent manner PMID: 27506849
42. What is less clear is how the spatial and temporal spread of intracellular Ca(2+) is shaped and regulated by differential expression of the individual SOCE genes and their splice variants, their heteromeric combinations and pre- and posttranslational modifications. This review focuses on principle mechanisms regulating expression, splicing, and targeting of Ca(2+) release-activated Ca(2+) (CRAC) channels. PMID: 26911279
43. The focus is on the activation of the STIM proteins, the subsequent coupling of STIM1 to Orai1, and the consequent structural rearrangements that gate the Orai channels into the open state to allow Ca(2+)permeation into the cell. [review] PMID: 26825122
44. Mutations in the STIM1 and ORAI1 genes cause calcium dyshomeostasis in tubular aggregate myopathy. (Review) PMID: 27879676
45. STIM1 knockdown did not alter proliferation or apoptosis, but promoted cell adhesion and inhibited migration and invasion in the gastric cancer cells. STIM1 knockdown did not alter the expression or phosphorylation of mitogen-activated protein kinase (MEK) or extracellular signal-regulated kinase (ERK), implying that STIM1 affected gastric cancer cell migration through a pathway independent of the MEK/ERK pathway. PMID: 27035326
46. SARAF overexpression attenuated store operated Ca2+ entry and the STIM1-Orai1 interaction in cells endogenously expressing STIM1 and Orai1 while RNAi-mediated SARAF silencing induced opposite effects. PMID: 27068144
47. Calsequestrin-1 monomers suppress Store-Operated Ca2+ Entry by interacting with STIM1 and attenuating STIM1 aggregation via its C-terminal amino acid 362-396. PMID: 27185316
48. the STIM1-Orai1 system has a role in intra-cellular calcium elevation induced by ATP in cultured human keratinocytes PMID: 26738614
49. Coupling of the endoplasmic reticulum (ER) Ca(2+)sensor, stromal interaction molecule 1 (STIM1), to the Ca(2+)-selective channel, Orai1, is regulated by these elements and depends on membrane organization, both at the plasma membrane and at the ER PMID: 27068962
50. STIM11-469 and Orai1 W76A mutants do not reduce channel open probability. PMID: 26809794
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相關疾病：

Immunodeficiency 10 (IMD10); Myopathy, tubular aggregate, 1 (TAM1); Stormorken syndrome (STRMK)
亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Cytoplasm, cytoskeleton. Sarcoplasmic reticulum.
組織特異性：

Ubiquitously expressed in various human primary cells and tumor cell lines.
數據庫鏈接：

HGNC: 11386

OMIM: 160565

KEGG: hsa:6786

STRING: 9606.ENSP00000300737

UniGene: Hs.501735

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