SP3 Antibody

中文名稱：

SP3兔多克隆抗體
貨號：

CSB-PA22589A0Rb
規格：

￥440
圖片：
IHC image of CSB-PA22589A0Rb diluted at 1:500 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) SP3 Polyclonal antibody
Uniprot No.：

Q02447
基因名：

SP3
別名：

D130027J01Rik antibody; DKFZp686O1631 antibody; GC binding transcription factor Sp 3 antibody; GC binding transcription factor Sp3 antibody; MGC105187 antibody; OTTMUSP00000014207 antibody; SP 3 antibody; Sp 3 transcription factor antibody; SP3 antibody; Sp3 transcription factor antibody; SP3_HUMAN antibody; Specificity protein 3 antibody; SPR 2 antibody; SPR-2 antibody; SPR2 antibody; Transcription factor SP 3 antibody; Transcription factor Sp3 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Transcription factor Sp3 protein (301-384AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，SP3 Antibody (CSB-PA22589A0Rb)，的標記方式是Non-conjugated。對于SP3 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA22589B0Rb	SP3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA22589C0Rb	SP3 Antibody, FITC conjugated
Biotin	CSB-PA22589D0Rb	SP3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
基因功能參考文獻：
1. expression of the nearby cyclindependent kinase inhibitor 1C (CDKN1C) gene was revealed to be upregulated after SP3 knockdown in cells that possessed non-risk alleles. This suggests that CDKN1C is potentially one of the functional targets of SNP rs163184, which modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a PMID: 29207083
2. High SP3 expression is associated with hepatocellular carcinoma. PMID: 28844709
3. we established a new regulatory model of COL1A1 regulation by HIF-1, and bring out its relationship with Sp3 transcription factor. In a fundamental level, these findings give insights in the mechanisms controlling COL1A1 gene expression. PMID: 27521280
4. Data indicate that the antitumor activity of trefoil factor 2 (TFF2) is mediated by an interaction with the transcription factor Sp3 (Sp3) in gastric cancer cells. PMID: 27668303
5. demonstrate that a 186bp region is the minimal essential region and that Sp3-GC1 binding is essential to the basal expression of KLF5 PMID: 27940107
6. Sp3 was found to be a major regulator of BNIP3 in prostate cancer. PMID: 26012884
7. Chromosome 2q31.1 is located between the genes that code for Sp3 transcription factor, which regulates expression of genes linked to glomerular function and the pathogenesis of nephropathy, and the CDCA7 transcription factor. PMID: 24029427
8. SP3 interacts with TNIP1 promoter and contributes to its basal and inducible expression. PMID: 23464785
9. Findings indicate a role for transcription factor Sp3 in sarcomas as a driver for expression of the metastasis-related gene AFAP1L1 (actin filament-associated protein 1-like 1). PMID: 23326307
10. transcription factor Sp3 acts to reduce the expression of many genes with Sp3 binding sites in their promoters by inhibiting the transition of paused RNA PolII to productive elongation. PMID: 23401853
11. Transcriptional control of the multi-drug transporter ABCB1 by transcription factor Sp3 in different human tissues PMID: 23133566
12. AP-1 and Sp3 are key regulators of IL-1beta-mediated modulation of xylosyltransferase I expression. PMID: 23223231
13. this study indicated that Sp3 was a major regulator of TFF2 expression. PMID: 23000412
14. Sp1 or Sp3 siRNA knockdown reduced 1alpha,25-dihydroxyvitamin D3-regulated hCYP24A1 promoter activity. PMID: 22871965
15. Extracellular signal-regulated kinase mitogen-activated protein kinase-dependent SOCS-3 gene induction requires c-Jun, signal transducer and activator of transcription 3, and specificity protein 3 transcription factors. PMID: 22311708
16. the two factors Klf4 and Sp3 exert an overlapping repressor function through their binding to the Notch1 promoter. PMID: 20442780
17. results suggest that Sp3 plays a key role in the expression of NOX4 in various cell lineages in humans. PMID: 21235713
18. Transcription of the transforming growth factor beta activating integrin beta8 subunit is regulated by SP3, AP-1, and the p38 pathway PMID: 20519498
19. human proximal FOXP3 promoter is controlled by activation through the TCR involving PKC and the NF-kappaB subunit p65 and by inhibition through a negative feedback loop and SP3. PMID: 20462637
20. Sp3- mediated transcriptional repression is due, at least in part, to competition for promoter-specific transcription factors PMID: 11773047
21. Results suggest that Sp1 and Sp3 associate with the hTERT promoter, recruiting HDAC for the localized deacetylation of nucleosomal histones and transcriptional silencing of the hTERT gene in normal human somatic cells. PMID: 12151407
22. CK2 phosphorylation of HDAC2 recruited by Sp1 or Sp3 could regulate HDAC activity and alter the balance of histone deacetylase and histone acetyltransferase activities and dynamic chromatin remodeling of estrogen-regulated genes. PMID: 12176973
23. This protein plays a role in the identification of regulatory elements in the human adipose most abundant gene transcript-1(apM-1) promoter. PMID: 12378384
24. 5' UTR of Sp3 transcription factor PMID: 12411611
25. This protein and Sp1 are involved in up-regulation of human deoxyribonuclease II transcription during differentiation of HL-60 cells. PMID: 12694199
26. Acetylation acts as a switch that controls the repressor and activator role of Sp3. PMID: 12837748
27. TFIIB intewracts with SP1/SP3 at the SP1 site. PMID: 12972613
28. In conclusion, we provide evidence for AML-1, PU.1, and Sp3 cooperatively and directly mediating BPI-expression during myeloid differentiation. PMID: 14623259
29. SP3 acts as a positive regulator on the core promoter of human ZPK gene PMID: 14697235
30. S-nitrosoglutathione increases Sp3 binding to DNA and transcription of CFTR at physiological concentrations, but inhibits Sp3 binding and CFTR transcription at nitrosative stress levels. PMID: 14766015
31. An SP3 binding site in the IGFBP4 gene was identified and the role of SP3 in regulating its promoter activity in CaCo-2 cells was studied. PMID: 14767471
32. Sp3 isoform ratios and activity are controlled at the translational level, which regulates expression of genes during mitosis and has effects on cell cycle regulation and tumorigenesis PMID: 14767558
33. Sp3 binding is regulated by methylation in the core-promoter region of the chondromodulin-I gene PMID: 15107420
34. four isoforms derive from alternative translational start sites at positions 1, 37, 856, and 907; the transcriptional activity of all the Sp3 isoforms is regulated by SUMO modification PMID: 15247228
35. Sp3 isoforms are sumoylated in vivo and this post-translational modification plays an important role in the regulation of Sp3-mediated transcription. PMID: 15494207
36. SP3 protein negatively regulates beta myosin heavy chain gene expression during skeletal muscle inactivity. PMID: 15572681
37. 10 genes were down-regulated following treatment of the T-ALL cells with 0.15 and 1.5 microg/mL of metal ores at 72 h. DNA-binding transcriptional activator activity. PMID: 15747776
38. Sp3 is a strong activator of dopamine transporter transcriptional activity. PMID: 15816870
39. cytochrome b5 gene transcription is regulated by Sp3, GATA-6, and steroidogenic factor 1 in human adrenal NCI-H295A cells PMID: 15831526
40. Results indicate important role for Sp1, Sp3, and NF-Y in transcription regulation of Sp3 proximal promotor PMID: 16024108
41. Sp3 D-domain modulates its protein levels and activation of the p21(CIP1/WAF1) promoter PMID: 16081043
42. increased pAPCP promoter activity in the MCF10A cell line in response to DMBA treatment is mediated by Sp3 PMID: 16150893
43. Sp3 and Sp4 cooperatively interact with ERalpha to activate VEGFR2 PMID: 16574784
44. PADI3 expression is driven by Sp3 bindin to the promoter region PMID: 16671893
45. Sp3 and Sp1 transcription factors have a crutial role in the expression of Protein S gene PMID: 16672217
46. Sp3 binds to the gamma-ENaC promoter and Sp3 binding is enhanced by butyrate PMID: 17241874
47. Data show that increased generation of C18-ceramide by hCerS1 expression mediates the association and recruitment of deacetylated Sp3/HDAC1 to the hTERT promoter, resulting in local histone H3 deacetylation and repression of the promoter. PMID: 17548428
48. IL-1beta decreases type II TGFbeta receptor expression by inducing Sp3 via NFkappaB PMID: 18053089
49. Results show activation of the Sp3 proximal promoter upon overexpression of NF-1, c-Myb, B-Myb, c-Jun and c-Fos, and repression after overexpression of E2F/DP1. PMID: 18342022
50. Observation suggested that deficient expression of SP3 gene occurs in Chinese MS patients, and that the SP3 expression may correlate with the clinical manifestations of MS and play roles in its immunological pathogenesis. PMID: 18393243
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亞細胞定位：

Nucleus. Nucleus, PML body. Note=Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.
蛋白家族：

Sp1 C2H2-type zinc-finger protein family
組織特異性：

Ubiquitously expressed.
數據庫鏈接：

HGNC: 11208

OMIM: 601804

KEGG: hsa:6670

STRING: 9606.ENSP00000310301

UniGene: Hs.531587

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