SMG7 Antibody
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中文名稱：SMG7兔多克隆抗體
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貨號：CSB-PA842128LA01HU
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規(guī)格：￥440
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圖片：
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IHC image of CSB-PA842128LA01HU diluted at 1:600 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
IHC image of CSB-PA842128LA01HU diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA842128LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) SMG7 Polyclonal antibody
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Uniprot No.：
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基因名：SMG7
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別名：breast cancer-associated antigen SGA-56M antibody; C1orf16 antibody; EST1 like protein C antibody; EST1 telomerase component homolog C antibody; EST1-like protein C antibody; EST1C antibody; ever shorter telomeres 1C antibody; FLJ23717 antibody; hSMG-7 antibody; nonsense mediated mRNA decay factor (C. elegans) antibody; Protein SMG7 antibody; SGA56M antibody; SMG 7 antibody; SMG-7 homolog antibody; Smg7 antibody; SMG7_HUMAN antibody
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宿主：Rabbit
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反應(yīng)種屬：Human
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免疫原：Recombinant Human Protein SMG7 protein (694-809AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產(chǎn)品，SMG7 Antibody (CSB-PA842128LA01HU)，的標記方式是Non-conjugated。對于SMG7 Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, IHC, IF
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推薦稀釋比：
Application Recommended Dilution IHC 1:500-1:1000 IF 1:200-1:500 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.
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基因功能參考文獻：
- Nonsense-mediated mRNA decay (NMD) substrates with PTCs undergo constitutive SMG6-dependent endocleavage, rather than SMG7-dependent exonucleolytic decay. In contrast, the turnover of NMD substrates containing upstream ORFs and long 3' UTRs involves both SMG6- and SMG7-dependent endo- and exonucleolytic decay, respectively; extent to which SMG6 and SMG7 degrade NMD substrates is determined by the mRNA architecture. PMID: 28461625
- Transcriptome-wide identification of nonsense-mediated mRNA decay-targeted human mRNAs reveals extensive redundancy between SMG6- and SMG7-mediated degradation pathways. PMID: 27864472
- We showed SMG7 mRNA levels in peripheral blood mononuclear cells correlated inversely with antinuclear antibody titres of patients with systemic lupus erythematosus. The inverse relationship between levels of the risk allele-associated SMG7 mRNAs and antinuclear antibody suggested the novel contribution of mRNA surveillance pathway to systemic lupus erythematosus pathogenesis. PMID: 26783109
- Depletion of nonsense-mediated mRNA decay pathway components Upf1, Smg5, and Smg7 led to increased levels of viral proteins and and virus release. PMID: 25211080
- The study demonstrates that SMG5-SMG7 and SMG6 exhibit different and non-overlapping modes of UPF1 recognition, thus pointing at distinguished roles in integrating the complex nonsense-mediated mRNA decay interaction network. PMID: 25013172
- Data show that phosphorylated hUPF1, the human ortholog of UPF1/SMG-2, forms a complex with human orthologs of the Caenorhabditis elegans proteins SMG-5 and SMG-7. PMID: 14636577
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亞細胞定位：Cytoplasm. Nucleus. Note=Predominantly cytoplasmic, and nuclear. Shuttles between nucleus and cytoplasm.
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數(shù)據(jù)庫鏈接：
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