SEMA4B Antibody
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中文名稱：SEMA4B兔多克隆抗體
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貨號：CSB-PA868280LA01HU
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規格：￥440
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圖片：
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Western Blot
Positive WB detected in: Rat luing tissue
All lanes: SEMA4B antibody at 6.3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 93, 82 kDa
Observed band size: 93 kDa -
IHC image of CSB-PA868280LA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
IHC image of CSB-PA868280LA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA868280LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) SEMA4B Polyclonal antibody
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Uniprot No.：
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基因名：SEMA4B
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別名：SEMA4B antibody; KIAA1745 antibody; SEMAC antibody; UNQ749/PRO1480 antibody; Semaphorin-4B antibody; Semaphorin-C antibody
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宿主：Rabbit
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反應種屬：Human, Rat
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免疫原：Recombinant Human Semaphorin-4B protein (163-253AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產品，SEMA4B Antibody (CSB-PA868280LA01HU)，的標記方式是Non-conjugated。對于SEMA4B Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:100-1:300 IF 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
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基因功能參考文獻：
- SEMA4B may induce FoxO1 nuclear retention through suppressing PI3K/Akt signaling pathway, which subsequently inhibited cell growth through the direct nuclear target of FoxO1, p21. PMID: 25746385
- Decrease in SEMA4b levels are associated with metastasis of non-small cell lung cancer. PMID: 25095981
- showed that HIF-1alpha recognized a hypoxia-responsive element (HRE) of SEMA4B gene, which is required for HIF-1-repressed SEMA4B expression PMID: 24474252
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亞細胞定位：Membrane; Single-pass type I membrane protein.
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蛋白家族：Semaphorin family
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數據庫鏈接：
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