RFX1 Antibody

中文名稱：

RFX1兔多克隆抗體
貨號：

CSB-PA019610LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate
All lanes: RFX1 antibody at 3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 105 kDa
Observed band size: 105 kDa

IHC image of CSB-PA019610LA01HU diluted at 1:700 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA019610LA01HU diluted at 1:700 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) RFX1 Polyclonal antibody
Uniprot No.：

P22670
基因名：

RFX1
別名：

AI047719 antibody; AI385641 antibody; EF-C antibody; EFC antibody; Enhancer factor C antibody; MHC class II regulatory factor RFX antibody; MHC class II regulatory factor RFX1 antibody; Regulatory factor X 1 antibody; Regulatory factor X, 1 (influences HLA class II expression) antibody; Regulatory factor X1 antibody; RFX antibody; RFX-1 antibody; Rfx1 antibody; RFX1_HUMAN antibody; Trans-acting regulatory factor 1 antibody; Transcription factor RFX1 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human MHC class II regulatory factor RFX1 protein (36-133AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，RFX1 Antibody (CSB-PA019610LA01HU)，的標記方式是Non-conjugated。對于RFX1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA019610LB01HU	RFX1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA019610LC01HU	RFX1 Antibody, FITC conjugated
Biotin	CSB-PA019610LD01HU	RFX1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter.
基因功能參考文獻：
1. RFX1 interacts with the D sequence of adeno-associated virus inverted terminal repeat and regulates AAV transduction. PMID: 29317724
2. RFX1 expression was significantly lower in HCC tissues compared to the corresponding non-tumor tissues. In vitro studies suggested that knocking down RFX1 facilitated HCC cell invasion, while overexpression of RFX1 reduced the invasion of HCC cells. RFX1 regulated expression of some epithelial-mesenchymal transition markers. PMID: 29764705
3. Findings indicate that RFX1 functions downstream of STAT3 and phosphorylated STAT3 can inhibit RFX1 expression and suggest a unique role for RFX1 in Th17-related autoimmune diseases. PMID: 29422534
4. Results show that reduced miRNA-124 improved microglia activation and up-regulated its downstream target RFX1, indicating that RFX1, as one of the target transcripts, facilitates microglial activation by miR-124 regulation; identified the binding site through which RFX1 directly regulates ApoE, may explain the missing link between decreased miRNA-124 and ineffective Abeta uptaking during aging. PMID: 28003160
5. induction of ciliogenesis increases the expression of RFX and dyslexia candidate genes PMID: 27451412
6. Data show that transcription factor regulatory factor X 1 (RFX1) protein expression can be tightly regulated by polyubiquitination-mediated proteosomal degradation via STIP1 homology and U-box containing protein 1 (STUB1). PMID: 27283392
7. therapeutic targets for childhood severe asthmatics identified thru DNA microarray PMID: 25979195
8. Data indicate that regulatory factor X transcription factor RFX-1 regulates SC-2001-mediated SHP-1 Phosphatase transcription in hepatocellular carcinoma cells. PMID: 24952874
9. regulatory factor 1 directly regulates CD44 expression in glioblastoma PMID: 24526308
10. Taken together, this study suggests ciliogenic RFX transcription factors regulate FGF-1B promoter activity and the maintenance of F1BGFP(+) NSPCs and GBM-SCs. PMID: 22415835
11. RFX1 reduces cell proliferation through inhibiting the TGFbeta2-ERK signaling pathway. RFX1 blocks TGFbeta2 expression through its direct action on TGFbeta2 transcription. PMID: 22582395
12. NLRC5-mediated histocompatibiility class I gene induction requires the W/S and X1, X2 cis-regulatory elements. PMID: 22490869
13. RFX1 recruits SUV39H1 to the promoter regions of the CD11a and CD70 genes in CD4(+) T cells, thereby regulating local H3K9 tri-methylation levels. PMID: 21192791
14. regulates autoimmunity via epigenetic modifications in T cells of systemic lupus erythematosus patients PMID: 20223637
15. We show binding of RFX proteins to an evolutionarily conserved X-box in the ALMS1 proximal promoter and present evidence that these proteins are responsible for ALMS1 transc PMID: 20381594
16. RFX1 may negatively regulate the self-renewal of GBM-SCs through modulating FGF-1B and FGF1 expression levels by binding the 18-bp cis-elements of the F1B promoter. PMID: 20189986
17. Data show that two regulatory factor for X box (RFX1 and 3) binding sites in exon1 of both the mouse and human microtubule-associated protein (MAP1A) gene are important for effective transcriptional repression in non-neuronal cells. PMID: 12411430
18. Disruption of the RFX site within 263P blunts repressor activity in transfected GC cells; however, repression is only abolished when both PSE-A/RFX and PSE-B/NF-1 sites are mutated. PMID: 12624117
19. novel role for the RFX family of transcription factors as modulators of Ras signalling in epithelial cells PMID: 15024578
20. RFX1 gene may be epigenetically silenced in human gliomas and involved in glioma tumorigenesis. PMID: 15334059
21. Six novel S'-Y' regulatory modules have been identified that are controlled by the human major histocompatibility class II-specific regulatory factor RFX complex. PMID: 15528357
22. evidence for a common mechanism for Crt1 and Rfx1 expression and for the conservation of their mode of action in response to a DNA replication block in yeasts and humans PMID: 16287876
23. The binding of alpha Adducin to RFX-I and their nuclear co-localization suggests that Adducin can have a role in modulating the transcriptional regulating activity of RFX-I. PMID: 16289097
24. results demonstrate that RFX1 and RFX5 differentially interact with class I HDACs underlying the different pathways when repressing collagen synthesis PMID: 16464847
25. RFX1 mediates the immediate early response of the Id2 gene by serum stimulation and suggest that the function of RFX1 is regulated intramolecularly in its suppression in growth-arrested cells. PMID: 17630394
26. RFX1 specifically bind to promoter region and negatively regulate the transcription of the human PNRC gene. PMID: 19334528
27. RFX and/or enhanceosome assembly plays a key role independent of transcriptional coactivator CIITA in protecting major histocompatibility class II antigen promoters against DNA methylation. PMID: 19620312
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亞細胞定位：

Nucleus.
蛋白家族：

RFX family
數據庫鏈接：

HGNC: 9982

OMIM: 600006

KEGG: hsa:5989

STRING: 9606.ENSP00000254325

UniGene: Hs.655215

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