RFC1 Antibody

說明書

中文名稱：

RFC1兔多克隆抗體
貨號：

CSB-PA005621
規格：

￥1090
圖片：
Western Blot analysis of K562 cells using RFC1 Polyclonal Antibody.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P35251
基因名：

RFC1
別名：

A1 140 kDa subunit antibody; A1 antibody; A1 P145 Activator 1 large subunit antibody; Activator 1 140 kDa subunit antibody; Activator 1 large subunit antibody; Activator 1 subunit 1 antibody; DNA binding Protein PO GA antibody; DNA-binding protein PO-GA antibody; MHC binding factor beta antibody; MHCBFB antibody; PO GA antibody; RECC1 antibody; Replication factor C (activator 1) 1, 145kDa antibody; Replication factor C 140 kDa subunit antibody; Replication factor C antibody; Replication factor C large subunit antibody; Replication factor C subunit 1 antibody; Replication factor C1 antibody; RF-C 140 kDa subunit antibody; RFC antibody; RFC1 antibody; RFC1_HUMAN antibody; RFC140 antibody; RFC140 Replication Factor C 140 kDa subunit antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Synthesized peptide derived from the C-terminal region of Human RFC1.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
ELISA	1:20000

Protocols：

Western Blotting (WB) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.; Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
基因功能參考文獻：
1. Human CTF18-RFC clamp-loader complexed with non-synthesising POLE efficiently loads the PCNA sliding clamp. PMID: 28199690
2. RFC depletion had a negative impact on LANA's ability to replicate and maintain viral DNA in cells containing artificial KSHV episomes or in infected cells, leading to loss of virus. PMID: 25071216
3. phosphorylation of serine 51 on hLigI plays a critical role in regulating the interaction between hLigI and RFC, which is required for efficient DNA replication and repair. PMID: 22952233
4. report that the large subunit (RFC1) of replication factor C is cleaved in Hutchinson-Gilford progeria syndrome cells, leading to the production of a truncated RFC1 of ~ 75 kDa PMID: 22168243
5. We identified a variant in each of 3 genes (RFC1, SCN1A, ANPEP) potentially implicated in susceptibility to severe neurological complications in West Nile Virus disease. PMID: 21881118
6. Small interfering RNA-mediated knockdown of RFC1 impairs upstream removal of UV lesions and abrogates the downstream recruitment of DNA polymerase delta. PMID: 20713449
7. analysis of the DNA-bound BRCA1 C-terminal region structure from human replication factor C p140 and model of the protein-DNA complex PMID: 20081198
8. interaction with histone deacetylase HDAC1 PMID: 12045192
9. RFC (p140) has an important role as a regulator of transcription and NF-kappaB activity PMID: 12509469
10. destabilization of the RF-C complex by CDKs may inactivate the RF-C complex at the end of S phase PMID: 12947101
11. NMR resonance assignments of the BRCT region of the large subunit PMID: 15772763
12. Ctf18-RFC interacts physically with pol eta, which allows DNA replication forks to overcome interference by various template structures, including damaged DNA and DNA-protein complexes that maintain chromosome cohesion PMID: 17545166
13. Data show that replication factor C (RFC), via several stimulatory motifs per molecule, potently activates flap endonuclease 1 (FEN1), making RFC a critical partner with FEN1 for the processing of eukaryotic Okazaki fragments. PMID: 19208620
14. a mutant version of hLigI, which mimics the hyperphosphorylated M-phase form of hLigI, does not interact with and is not inhibited by RFC, demonstrating that inhibition of ligation is dependent upon the interaction between hLigI and RFC PMID: 19223468
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亞細胞定位：

Nucleus.
蛋白家族：

Activator 1 large subunit family
組織特異性：

Wide tissue distribution. Undetectable in placental tissue.
數據庫鏈接：

HGNC: 9969

OMIM: 102579

KEGG: hsa:5981

STRING: 9606.ENSP00000371321

UniGene: Hs.507475

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