RAB35 Antibody

中文名稱：

RAB35兔多克隆抗體
貨號：

CSB-PA614887LA01HU
規格：

￥440
圖片：
Immunofluorescence staining of Hela cells with CSB-PA614887LA01HU at 1:185, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA614887LA01HU diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA614887LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) RAB35 Polyclonal antibody
Uniprot No.：

Q15286
基因名：

RAB35
別名：

GTP binding protein RAY antibody; GTP-binding protein RAY antibody; H ray antibody; OTTHUMP00000240256 antibody; OTTHUMP00000240257 antibody; OTTHUMP00000240258 antibody; OTTHUMP00000240259 antibody; RAB1C antibody; Rab35 antibody; RAB35 member RAS oncogene family antibody; RAB35; member RAS oncogene family antibody; RAB35_HUMAN antibody; Ras related protein rab 1c (GTP binding protein ray) antibody; Ras related protein Rab 1C antibody; Ras related protein Rab35 antibody; Ras-related protein Rab-1C antibody; Ras-related protein Rab-35 antibody; RAY antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Ras-related protein Rab-35 protein (1-201AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，RAB35 Antibody (CSB-PA614887LA01HU)，的標記方式是Non-conjugated。對于RAB35 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA614887LB01HU	RAB35 Antibody, HRP conjugated	ELISA
FITC	CSB-PA614887LC01HU	RAB35 Antibody, FITC conjugated
Biotin	CSB-PA614887LD01HU	RAB35 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.
基因功能參考文獻：
1. A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration. PMID: 29662076
2. The results provide evidence that MICAL1 plays an essential role in the activation of ROS/Akt signaling and cell invasive phenotype and identify a novel link between RAB35 and MICAL1 in regulating breast cancer cell invasion. PMID: 27430308
3. The involvement of Rab35 in diverse and apparently unrelated cellular functions can be explained by the central role of this GTPase in regulating phosphoinositides and F-actin, both on endosomes and at the plasma membrane[review]. PMID: 27329675
4. Data suggest that Rab35, interacting with TBC1D10A, functions in vascular endothelial cells as a negative regulator of histamine-evoked, Ca2+-dependent Weibel-Palade body exocytosis, most likely acting through the downstream effectors ACAP2 and Arf6. (Rab35 = rab GTP-binding protein 53; TBC1D10A = TBC1 domain family member 10A; ACAP2 = centaurin beta2; Arf6 = ADP-ribosylation factor 6) PMID: 28566286
5. Short-term EGF stimulation if of lung tumor cells can increase the interaction between RUSC2 and GIT2, prolonged stimulation leads to a decrease of their interaction through activating Rab35. PMID: 27238570
6. Here the authors report that EspG interacts specifically with the small GTPases ARF6 and Rab35 during infection. PMID: 27261256
7. Authors propose that the precise spatial and temporal activation of Rab35 acts as a major switch for OCRL recruitment on newborn endosomes, post-scission PtdIns(4,5)P2 hydrolysis, and subsequent endosomal trafficking. PMID: 26725203
8. Rab35 serves as a clathrin-mediated endocytosis detector. Loss of Rab35 input leads to elevated Arf6-GTP and shifts the sorting of clathrin-independent endocytosis cargo proteins to lysosomes. PMID: 25988331
9. the activation-inactivation cycles of Rab35 and ARF6 are required for the uptake of zymosan and that ACAP2 is an important component that links Rab35/ARF6 signaling during phagocytosis of zymosan. PMID: 26229970
10. Two somatic RAB35 mutations found in human tumors generate alleles that constitutively activate PI3K/AKT signaling, suppress apoptosis, and transform cells in a PI3K-dependent manner. PMID: 26338797
11. mutual regulation with arf6 of cell adhesion and migration PMID: 23264734
12. Rab35 acts as a downstream target of Dvl2 and mediates cell migration. PMID: 23353182
13. Activated ARF6 reduces Rab35 loading into the endocytic pathway. PMID: 22226746
14. whereas connecdenn 1 and 2 activate Rab35 for endosomal trafficking, connecdenn 3 uniquely activates Rab35 for its role in actin regulation PMID: 22072793
15. analysis of DENND1B-S complexed with its substrate Rab35 PMID: 22065758
16. the phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) 5-phosphatase OCRL, which is mutated in Lowe syndrome patients, is an effector of the Rab35 GTPase in cytokinesis abscission PMID: 21706022
17. A Small Ras-like GTPase protein Ray was indicated to modulate p53 transcriptional activity of PRPK. PMID: 16600182
18. localized to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway; plays an essential role during the terminal steps of cytokinesis PMID: 16950109
19. EPI64C and Rab35 regulate a recycling pathway in T cells and contribute to immunological synapse formation, most likely by participating in TCR transport to the immunological synapse PMID: 18450757
20. study found that Rab35 regulates the assembly of actin filaments in filopodia formation; effect was mediated by the actin-bundling protein fascin, which directly associated with active Rab35 PMID: 19729655
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亞細胞定位：

Cell membrane; Lipid-anchor; Cytoplasmic side. Membrane, clathrin-coated pit. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome. Melanosome.
蛋白家族：

Small GTPase superfamily, Rab family
數據庫鏈接：

HGNC: 9774

OMIM: 604199

KEGG: hsa:11021

STRING: 9606.ENSP00000229340

UniGene: Hs.524788

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