RAB27A Antibody
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中文名稱：RAB27A兔多克隆抗體
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貨號：CSB-PA946810
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規格：￥1100
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圖片：
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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA946810(RAB27A Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) -
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: K562 cells, Primary antibody: CSB-PA946810(RAB27A Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
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其他：
產品詳情
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Uniprot No.：
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基因名：RAB27A
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別名：GS2 antibody; GTP-binding protein Ram antibody; HsT18676 antibody; MGC117246 antibody; Mutant Ras related protein Rab-27A antibody; Rab-27 antibody; RAB-27A antibody; RAB27 antibody; RAB27A antibody; RAB27A member RAS oncogene family antibody; RAM antibody; Ras-related protein Rab-27A antibody; Ras-related protein Rab27A antibody; RB27A_HUMAN antibody
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宿主：Rabbit
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反應種屬：Human,Mouse,Rat
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免疫原：Fusion protein of Human RAB27A
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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抗體亞型：IgG
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純化方式：Antigen affinity purification
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
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產品提供形式：Liquid
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應用范圍：ELISA,WB,IHC
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推薦稀釋比：
Application Recommended Dilution ELISA 1:2000-1:5000 WB 1:500-1:2000 IHC 1:25-1:100 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
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基因功能參考文獻：
- These results collectively suggest that the active form of Rab27a enhances human parainfluenza virus type 2 growth by promoting transport of F and HN proteins to the plasma membrane. PMID: 29374787
- A novel Rab27a mutation binds melanophilin, but not Munc13-4, causing immunodeficiency without albinism. PMID: 27016801
- We reported the first patient with GS2 in Thailand. The patient was compound heterozygous for two novel mutations, c.109A>T (p.K37X) and c.318T>G (p.S106R). We reported the first case of a successful prenatal diagnosis for GS2. The result revealed that the fetus carried the p.K37X, but not the p.S106R (Fig. C), suggesting that the fetus would be unaffected with GS2. PMID: 28484936
- Findings indicate that rab GTP-binding proteins Rab27A and Rab27B play significant roles in cell invasion, proliferation, and apoptosis, as well as in chemotherapy resistance. PMID: 28902788
- Here, we report on a new RAB27A genetic anomaly observed in seven Saudi Arabia families that had remained negative after extensive molecular genomic DNA testing. Linkage analysis and targeted sequencing of the RAB27A genomic region in several of these patients led to the identification of a common homozygous tandem duplication of 38 kb affecting exon 2-5 and resulting in a premature stop codon PMID: 28585352
- In comparison with the normal tissues, miR-182-5p is expressed at a higher level in gastric cancer (GC) tissues, while RAB27A is expressed at a lower level in cancerous tissues. The down-regulation of miR-182-5p and up-regulation of RAB27A can significantly decrease the viability, migration, invasion, and mitosis of HGC-27 cells. PMID: 28546229
- Rab27A is mediated by NF-kappaB and promotes stemness of colon cancer cells via up-regulation of cytokine secretion PMID: 27556511
- These results demonstrated that miR-145 has an inhibitory role in TNBC malignancy by targeting MMP11 and Rab27a, which might be potential therapeutic and diagnostic targets for TNBC. PMID: 27364572
- These signaling pathways can trigger several secretion modes including single WPB release and multigranular exocytosis. In this review we will give an overview of the WPB lifecycle from biogenesis to secretion and we will discuss several deficiencies that affect the WPB lifecycle. [review] PMID: 28004844
- RAB27A, RAB27B and VPS36 are frequently underexpressed in advanced prostate cancer and are inversely correlated with prostate cancer outcome. There seems to be a close relationship in the expression of RAB27A, RAB27B and VPS36, with RAB27A and RAB27B being dependent on VPS36. PMID: 28197629
- identified small molecule inhibitors of Rab27a-JFC1 binding that were also active in cell-based neutrophil-specific exocytosis assays, demonstrating the druggability of Rab GTPases and their effectors. PMID: 27702998
- MicroRNA-134-3p is a novel potential inhibitor of human ovarian cancer stem cells by targeting RAB27A. PMID: 28043921
- decreased expression of Rab27A and Rab27B, especially Rab27A, closely correlated with tumor progression and are valuable prognostic indicators in colorectal cancer patients. PMID: 26760980
- We report two unrelated teenagers with hemophagocytic lymphohistiocytosis and an identical heterozygous RAB27A mutation (c.259G-->C). PMID: 26880764
- Data suggest that microRNA miR-582-5p may function as a tumor suppressor in the development of colorectal carcinoma (CRC) by targeting RAS-related GTP-binding protein (Rab27a), indicating a novel therapeutic strategy for patients with CRC. PMID: 26384136
- Experimental data are reviewed with a focus on the secretory Rab27 family of small GTPases and their implications in cancer progression. PMID: 23665896
- miR-122 has a Rab27a-dependent function in the hepatitis C virus lifecycle PMID: 26305877
- Rab27A may be used as a valuable prognostic biomarker for colorectal carcinoma patients. PMID: 26070933
- Our data indicate that RAB27A expression is an independent prognostic marker for pancreatic ductal adenocarcinoma PMID: 25428385
- This study identified a common variant located in RAB27A gene influencing FeNO levels specifically in adults and with a biological relevance to the regulation of FeNO levels. PMID: 25431337
- These studies highlight the need for RAB27A sequencing in patients with FHL with normal pigmentation and identify a critical binding site for Munc13-4 on Rab27a, revealing the molecular basis of this interaction. PMID: 25312756
- Data show that cystic fibrosis (CF) neutrophils release less secondary and tertiary granule components and that activation of the low-molecular-mass GTP-binding protein Rab27a, involved in the regulation of granule trafficking, is defective. PMID: 24934256
- Rab27a was identified as a prognostic biomaker by mRNA profiling, correlated with malignant progression and subtype preference in gliomas. PMID: 24587032
- Silencing of the exocytotic RAB family members RAB27A or RAB27B halted miR23b and miR921 secretion and reduced cellular invasion. PMID: 25261234
- EPI64, a candidate GAP that is specific for Rab27. PMID: 24673604
- Rab27a plays an important role in NET formation induced by both Candida albicans infection and PMA treatment by regulating ROS production. PMID: 24404184
- Recruitment of STXBP1 by the Rab27A effector SYTL4 promotes Weibel-Palade body exocytosis. PMID: 24700782
- The results showed that Rab27A(Q78L) is unable to localize on mature melanosomes and that its inhibitory activity on melanosome transport is completely dependent on its binding to the Rab27A effector Slac2-a/melanophilin. PMID: 24584932
- Exosomes derived from Rab27aoverexpressing cancer cells elicited more potent antitumor immune effects. PMID: 24146068
- several crystal structures of the myosin Va or the myosin Vb globular tail domain that gives insights into how the motor is linked to the recycling membrane compartments via Rab11 or the melanophilin adaptor that binds to Rab27a. PMID: 24248336
- Rab27a has a key role in eosinophil degranulation. Furthermore, these findings have implications for Rab27a-dependent eosinophil degranulation in airway inflammation. PMID: 23986549
- Defects in cargo trafficking caused by mutations in RAB27A and UNC13D genes, encoding Rab27a and its effector Munc13-4, cause severe immunodeficiencies in humans. (Review) PMID: 23810987
- Rab27a could improve cell viability, proliferation, and migration of U251 cells and inhibit its apoptosis by promoting secretion of cathepsin D and miR-124 suppression. PMID: 23553027
- Upregulation of the Rab27a-dependent lysosomal trafficking and secretory pathways contributes to the correction of some of the cellular defects induced by lysosomal overload in cystinosis, including endoplasmic reticulum stress. PMID: 23716592
- Altogether, these results indicate that Rab27a plays an important role in herpes simplex virus 1 infection of oligodendrocytic cells. PMID: 23164453
- RAB27A localization and function in melanocytes. [Review] PMID: 23176485
- the intrinsically essential role of RAB27A in human ethnic skin color determination PMID: 22844437
- Rab27 and Rab3 sequentially regulate human sperm dense-core granule exocytosis, and Rab27 is also required for the acrosome reaction. PMID: 22753498
- Upregulated expression of rab4, rab5, rab7, and rab27 correlates with antemortem measures of cognitive decline in individuals with mild cognitve impairment and Alzheimer disease. PMID: 21669283
- The pathologic defect in Griscelli syndrome 3 came from the MLPH R35W substitution, which induced aggregation of melanosomes in the perinuclear area of melanocytes because of failure to interact with RAB27A. PMID: 21883982
- A Rab27a/MyRIP/myosin Va complex is involved in linking von-Willebrand factor (Vwf) to the peripheral actin cytoskeleton of endothelial cells to allow full maturation and prevent premature secretion of vWF. PMID: 21740491
- Data show that point mutations in the binding motif of munc13-4 have severely impaired rab27a binding, allowing dissection of rab27a requirements in munc13-4 function. PMID: 21693760
- Rab27a hasa role in the CMV life cycle and CMV and LRO biogenesis share common molecular mechanisms PMID: 21170347
- Rab27a plays a direct regulatory role in the nascent process of phagocytosis by prolongation of the stage of actin coating via suppression of Coronin 1A. PMID: 21169636
- Molecular analysis revealed a novel homozygous mutation in exon 5, namely, a single-base substitution (g.42996 A>G) leading to an amino acid change (S115G) and thus confirming the diagnosis of Griscelli syndrome type 2. PMID: 21314004
- Loss of Rab27a increased the fraction of mobile lytic granules and the extent of their movement in the cytosol. PMID: 20877725
- TBC1D16 and RAB27A, were identified known drivers of melanoma both are involved in the regulation of vesicular trafficking, which highlights this process as important for proliferation in melanoma. PMID: 21129771
- The Rab27A(K22R) mutant normally binds Munc13-4, but not Slp2-a or Slp4-a, whereas the Rab27A(I44T) mutant shows reduced binding activity to Slp2-a and Munc13-4 but normally binds Slp4-a. PMID: 20370853
- RAB27A mutations were found in 1 of the 21 families with haemophagocytic syndromes without mutations in familial HLH (FHL) causing genes PMID: 19953648
- role in melanosome transport PMID: 11980908
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相關疾病：Griscelli syndrome 2 (GS2)
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亞細胞定位：Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome.
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蛋白家族：Small GTPase superfamily, Rab family
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組織特異性：Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
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