Phospho-UBTF (Ser484) Antibody
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中文名稱：磷酸化-UBTF (Ser484)兔多克隆抗體
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貨號：CSB-PA072052
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規格：￥2454
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圖片：
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Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using UBF (Phospho-Ser484) antibody. The lane on the right is treated with the synthesized peptide. -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using UBF (Phospho-Ser484) antibody. The picture on the right is treated with the synthesized peptide.
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) UBTF Polyclonal antibody
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Uniprot No.：
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基因名：
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別名：UBTF; UBF; UBF1; Nucleolar transcription factor 1; Autoantigen NOR-90; Upstream-binding factor 1; UBF-1
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宿主：Rabbit
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反應種屬：Human,Mouse,Rat
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免疫原：Peptide sequence around phosphorylation site of serine 484 (P-E-S(p)-P-K) derived from Human UBF.
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免疫原種屬：Homo sapiens (Human)
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克隆類型：Polyclonal
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純化方式：Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
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濃度：It differs from different batches. Please contact us to confirm it.
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產品提供形式：Liquid
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應用范圍：ELISA,WB,IHC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:3000 IHC 1:50-1:100 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
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基因功能參考文獻：
- TP53INP2 promotes ribosome biogenesis through facilitating rRNA synthesis at the nucleolus PMID: 27172002
- Study demonstrated that HMG box 1, the linker region between the HMG boxes and C-terminal acidic region (AR), and the AR cooperatively regulate the nucleolar localization of UBF. PMID: 28874518
- In seven unrelated affected individuals, all suffering from developmental regression starting at 2.5-7 years, the authors identified a heterozygous variant, c.628G>A in UBTF, encoding p.Glu210Lys in UBF, which occurred de novo in all cases. PMID: 28777933
- RINT-1 interacts with MSP58 and UBF within nucleoli and plays a role in ribosomal gene transcription. PMID: 27530925
- This study provides some compelling evidences in support of HBx-elicited and c-Myc-mediated increase in UBF levels that abets oncogenic onslaught in hepatic cells by increasing rDNA transcription and ribosome biogenesis. PMID: 25890091
- These results suggest that UBF can inhibit gene expression from viral DNA prior to its replication. PMID: 25965800
- we have uncovered a novel role for UBTF1 and UBTF2 in maintaining genome stability through coordinating the expression of highly transcribed Pol I (UBTF1 activity) and Pol II genes (UBTF2 activity). PMID: 25452314
- p14ARF fails to prevent E7-mediated UBF1 phosphorylation, but could facilitate nucleolar pRb inactivation by targeting E7 to the nucleolus. PMID: 24798431
- PIP2 depletion reduces Pol I transcription. PMID: 23591814
- UBF is expressed widely by human multiple fetal tissue,s and the expression level is very high in HL-60 cells. PMID: 21162312
- Downregulation of RUNX2 expression reduces the localization of HDAC1 to the nucleolar periphery and also decreases the association between HDAC1 and UBF. PMID: 22393235
- hALP binds the upstream binding factor (UBF) in vivo and in vitro PMID: 21177859
- depletion of upstream binding factor (UBF; an rRNA transcription factor) decreased the chromatin binding affinity of B23, which in turn led to an increase in histone density at the r-chromatin PMID: 20713446
- The three-dimensional solution structure of the first high mobility group (HMG) box domain in upstream binding factor has been determined by multidimensional NMR. PMID: 11969401
- we have identified an interaction between UBF and TAF1, a factor involved in the transcription of cell cycle and growth regulatory genes. Coimmunoprecipitation and protein-protein interaction assays confirmed that TAF1 binds to UBF. PMID: 12498690
- The DNA binding affinity of UBF's fifth box domain (HMG box 5) is found to be much weaker than that of the first HMG box domain (HMG box 1). PMID: 12590579
- Data show that both UBF1 and UBF2 activate RNA polymerase II-regulated, beta-catenin-responsive promoters. PMID: 12748295
- Proto-Oncogene Proteins c-myc activated transcription from the UBF promoter PMID: 15282543
- results suggest that extensive binding of UBF is responsible for formation and maintenance of the secondary constriction at active NORs, and that UBF mediates recruitment of the pol I machinery to nucleoli independently of promoter elements PMID: 15598984
- Data suggest that the A state of human upstream binding factor HMG Box1 could represent a potential folding intermediate on protein folding pathway. PMID: 15752694
- A potential protein-folding pathway is proposed for upstream binding factor HMG box 1 domain based on the early stages of its pH 2.1 unfolded state characterized by multidimensional heteronuclear magnetic resonance spectroscopy. PMID: 15924431
- SL1 directs preinitiation complex formation, functioning in core promoter binding, RNA polymerase I recruitment, and UBF stabilization. PMID: 15970593
- model for CAST/hPAF49 function in which the network of interactions of Pol I-specific subunits with UBF facilitates conformational changes of the polymerase, leading to stabilization of the Pol I-template complex and, thereby, activation of transcription PMID: 16809778
- UBF activates transcription in the transition between initiation and elongation, at promoter escape by RNA polymerase I (Pol I). PMID: 16858408
- Upon p14ARF overexpression, UBF was found hypophosphorylated, thus unable to efficiently recruit the transcription complex, these data define a new p53-independent pathway that could regulate cell cycle through the negative control of rRNA transcription. PMID: 16924243
- GdmCl-induced equilibrium unfolding transition of HMG box 5 of hUBF was monitored by both circular dichroism and fluorescence spectra. A cooperative two-state unfolding process was observed PMID: 17260958
- possible sites in hUBF HMG box 5 that may interact with the first bromodomain of TAF1 were proposed PMID: 17505112
- transcription factor UBF binds extensively across rDNA throughout the cell cycle, resulting in a specialized form of chromatin that is the hallmark of active nucleolar organizer regions PMID: 17699751
- found a small but significant difference between the emerging daughter cells in the number of UBF-loaded NORs PMID: 18502146
- The authors propose that UBF is recruited to the replication compartments to aid replication of HSV-1 DNA. PMID: 19088274
- The established differential localization of UBF in nucleoli of HeLa cells has a functional meaning. It reflects both the level of rRNA synthesis activities and the architectural role of UBF in nucleoli of these cells. PMID: 19393134
- these results implicate a fluid helix-turn-helix folding model of hUBF Box-5. PMID: 19452555
- central repeated domain of treacle binds with RNA polymerase I, while that the treacle C-terminus is involved in rDNA promoter recognition and UBF recruitment. PMID: 19527688
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亞細胞定位：Nucleus, nucleolus.
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