Phospho-PAK2 (S141) Antibody

說明書

中文名稱：

磷酸化-PAK2 (S141)兔多克隆抗體
貨號：

CSB-PA020222
規格：

￥1090
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q13177
基因名：

PAK2
別名：

C-t-PAK2 antibody; CB422 antibody; EC 2.7.11.1 antibody; Gamma PAK antibody; Gamma-PAK antibody; hPAK65 antibody; Kinase antibody; p21 (CDKN1A) activated kinase 2 antibody; p21 (CDKN1A)-activated kinase 2a antibody; p21 activated kinase 2 antibody; p21 protein (Cdc42/Rac)-activated kinase 2 antibody; p21 protein Cdc42 Rac activated kinase 2 antibody; p21-activated kinase 2 antibody; p21-activated kinase; 65-KD antibody; p21-activated protein kinase I antibody; p21CDKN1A activated kinase 2 antibody; p27 antibody; p34 antibody; p58 antibody; p65PAK antibody; PAK 2 antibody; PAK-2 antibody; PAK-2p34 antibody; Pak2 antibody; PAK2_HUMAN antibody; PAK65 antibody; PAKgamma antibody; S6 H4 kinase antibody; S6/H4 kinase antibody; Serine threonine protein kinase PAK 2 antibody; Serine/threonine protein kinase PAK 2 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Synthesized peptide derived from Human PAKγ around the phosphorylation site of S141.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, IHC, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:10000

Protocols：

Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation.
基因功能參考文獻：
1. Overexpression of PAK2 in oral squamous cell carcinomas may be associated with an advanced pathology grade. PMID: 29714078
2. the promotion of apoptosis by human cytomegalovirusmiRUS45p in cells was specifically mediated via inhibition of PAK2 expression. PMID: 28765936
3. leukaemic cells explicitly require PAK2 to grow towards an extracellular matrix. PAK2-deficient cells fail to form colonies in methylcellulose and to induce lymphomas in vivo. PAK2 might therefore be the critical isoform in leukaemic cells by controlling tumour growth PMID: 28707321
4. Results show that PAK2 kinase plays an alternative anti-apoptotic role, phosphorylating caspase-7 and promoting unfettered cell growth and chemotherapeutic resistance. PMID: 27889207
5. We found that overexpression of miR-137 inhibited the proliferation of melanoma cells, which could be phenocopied by knockdown of PAK2 using siRNAs. PMID: 26186482
6. PAK2 is a direct effector of TSC1-TSC2-RHEB signaling and a new target for rational drug therapy in TSC. PMID: 26412398
7. Nef exploits PAK2 in a stepwise mechanism in which its kinase activity cooperates with an adaptor function for the exocyst complex to inhibit host cell actin dynamics. PMID: 26350970
8. Cytoplasmic Pak2 may promote cell proliferation in normal endometrium during menstrual cycle. PMID: 26218748
9. Further analyses show that HDAC6 may promote growth of GBM cells through inhibition of SMAD2 phosphorylation to downregulate p21 PMID: 26150340
10. Findings indicate that repression of microRNA miR-134 and consequent up-regulation of p21-activated kinase 2 (Pak2) might contribute to paclitaxel resistance. PMID: 26363097
11. Inhibition of PAK activation at late G2-phase centrosomes caused by Rac1 inactivation coincides with impeded activation of Aurora A and the CyclinB/Cdk1 complex and delayed mitotic entry. PMID: 24840740
12. Results identified Pak2 as a possibly important mediator of ovarian cancer cell migration on extracellular matrix. PMID: 25050916
13. PAK2 activation may be associated with advanced tumor progression and poor prognosis of gastric cancer PMID: 24621074
14. Prostasin repress cancer cells and contribute to chemoresistance by modulating the CASP/PAK2-p34/actin pathway. PMID: 24434518
15. Authors demonstrate that HIV-1 Nef expression mediates phosphorylation of Mek1 on serine298 and Pak2 on serine192/197 in T cell lines as well as primary human T cells. PMID: 23746211
16. thrombin induces monocyte/macrophage migration via PAR1-Galpha12-dependent Pyk2-mediated Gab1 and p115 RhoGEF interactions, leading to Rac1- and RhoA-targeted Pak2 activation. PMID: 24025335
17. PAK2 negatively modulate TGF-beta signaling by attenuating the receptor-Smad interaction and thus Smad activation PMID: 22393057
18. Low-to-moderate penetrance protein coding mutations or non-coding mutations at DLG1 and/or PAK2, or a nearby gene, may reproduce the behavioral characteristics of the 3q29 microdeletion. PMID: 21850710
19. The ability of Nef to associate with PAK2 correlates with the ability to enhance HIV-1 replication. PMID: 21819585
20. highly expressed PAK2 mediates chemotherapeutic resistance in human breast invasive ductal carcinoma by negatively regulating caspase-7 activity PMID: 21555521
21. High PAK2 is associated with melanoma. PMID: 21177766
22. Mechanistic studies of the autoactivation of PAK2: a two-step model of cis initiation followed by trans amplification. PMID: 21098037
23. analysis of evolutionary conserved residues that are crucial for the catalytic activity of PKA and Pak2 PMID: 20209159
24. The association between the CD4 receptor and protein kinase pp58 and the protein-tyrosine kinase within the cell introduces a specific pathway by which T lymphocytes become activated. PMID: 20724730
25. MYO18A is a novel binding partner of the PAK2/betaPIX/GIT1 complex and suggest that MYO18A may play an important role in regulating epithelial cell migration via affecting multiple cell machineries. PMID: 19923322
26. Knockdown of PAK2 enhances loss of cell-cell junctions and increases lamellipodium extension but does not affect migration speed Hepatocyte Growth Factor (HGF) stimulated DU145 prostate carcinoma cells. PMID: 19628037
27. The enzymatic phosphorylation reaction of PAK2 can be best interpreted by a rapid-equilibrium random bi-bi reaction model; the catalysis reaction is partially limited by both the phosphoryl group transfer and the product release steps. PMID: 12549935
28. The opposing effects of Core protein on the transcription pf P21 might be important in the progression of liver disease in HCV-positive patients. PMID: 12823590
29. Caspase-activated PAK-2 is regulated by subcellular targeting and proteasomal degradation PMID: 12853446
30. sites of PAK2 autophosphorylation in the regulatory and the catalytic domains and their kinetic effect; multiple regions of PAK2 are involved in the enzyme-substrate recognition PMID: 12907671
31. Pak2 phosphorylates Myc at three sites (T358, S373, and T400) and affects Myc functions both in vitro and in vivo PMID: 14749374
32. Nef induces signal transduction via the recruitment of a signaling machinery including Pak2 into lipid rafts, thereby mimicking a physiological cellular mechanism to initiate the TCR cascade. PMID: 15047825
33. PAK2 kinase activity is increased in response to TCR stimulation; results suggest a novel role for PAK2 as a positive regulator of T cell activation. PMID: 15187108
34. interacts with Nef proteins from SIV infected chimpanzees PMID: 15194762
35. Pak2 has a role in the down-regulation of translation initiation in apoptosis by phosphorylation of Mnk1 PMID: 15234964
36. PS-GAP is a novel regulator of caspase-activated PAK-2 PMID: 15471851
37. PAK-2 is activated in 1-LN prostate cancer cells by a proteinase inhibitor, alpha 2-macroglobulin PMID: 15908432
38. binding of Cdc42 localizes Pak2 to the endoplasmic reticulum, where autophosphorylation alters association of the two proteins PMID: 16204230
39. Pak2 binds to and phosphorylates initiation factor eIF4G, which inhibits association of eIF4E with m7GTP, reducing translation initiation. PMID: 16281055
40. Nef protein amino acids at positions 85, 89, 187, 188, and 191 (L, H, S, R, and F in the clade B consensus, respectively) are critical for Pak2 association and activation PMID: 16501114
41. Posttranslational myristoylation of PAK2 might be part of a unique series of mechanisms involved in the regulation of the later events of apoptosis. PMID: 16617111
42. c-Abl represents a target downstream of phosphatidylinositol 3-kinase-activated PAK2, which differentiates TGF-beta signaling in fibroblasts and epithelial cell lines. PMID: 16867995
43. This study of tissue-derived HIV-1 Nefs demonstrates that CD4 and MHC-I downregulation are highly conserved Nef functions, while Pak2 association is variable in late stage AIDS patients. PMID: 16979207
44. define a new class of PAK-interacting proteins, which play an important role in actin cytoskeletal reorganization PMID: 17543336
45. interaction of Nef with PAK2 does not play a major role in T-cell activation, viral replication, and apoptosis. PMID: 17881449
46. protein phosphatase 1alpha can act directly on phosphorylated Thr-402 in the activation loop of PAK2 and down-regulate its kinase activity PMID: 18176785
47. Data show RNAi-mediated or dominant-negative suppression of Pak2, major regulators of cytoskeletal signaling downstream of Cdc42 or Rac1, markedly inhibits EC lumen and tube formation. PMID: 18319301
48. Huntingtin exerts anti-apoptotic effects by binding to Pak2, which reduces the abilities of caspase-3 and caspase-8 to cleave Pak2 and convert it into a mediator of cell death. PMID: 19240112
49. PAK-2 activity controls the apoptotic response by regulating levels of activated caspase 3 and thereby its own cleavage to the proapoptotic PAK-2p34 fragment PMID: 19242610
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亞細胞定位：

[Serine/threonine-protein kinase PAK 2]: Cytoplasm. Note=MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.; [PAK-2p34]: Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Note=Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
蛋白家族：

Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
組織特異性：

Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
數據庫鏈接：

HGNC: 8591

OMIM: 605022

KEGG: hsa:5062

STRING: 9606.ENSP00000314067

UniGene: Hs.518530

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