Phospho-ELK1 (Ser389) Antibody

說明書

中文名稱：

磷酸化-ELK1 (Ser389)兔多克隆抗體
貨號：

CSB-PA239814
規格：

￥2454
圖片：
Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using Elk1(Phospho-Ser389) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1(Phospho-Ser389) Antibody(left) or the same antibody preincubated with blocking peptide(right).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) ELK1 Polyclonal antibody
Uniprot No.：

P19419
基因名：

ELK1
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Peptide sequence around phosphorylation site of serine 389 (P-R-S(p)-P-A) derived from Human Elk-1.
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
濃度：

It differs from different batches. Please contact us to confirm it.
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:1000
IHC	1:50-1:100

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation.
基因功能參考文獻：
1. enhanced ETS factor activity and the transcription of ETS family target genes related to spliceosome function and cell death induction via alternate MCL1 splicing, is reported. PMID: 29118074
2. Authors found that miR-185-5p decreases HBV S1p activity by targeting ELK1. PMID: 30308183
3. High ELK1 expression is associated with thyroid cancer progression. PMID: 30015900
4. ID1, CTCF and ELK1 may be associated with prostate cancer, and may be potential therapeutic targets for the treatment of this disease. PMID: 29956775
5. Study demonstrates that miR-135a regulates cell proliferation in breast cancer by targeting ELK1 and ELK3 oncogenes, and suggests that miR-135a potentially can act as a tumor suppressor. PMID: 29892795
6. Phospho-ELK1 overexpression serves as a predictor of poor prognosis in patients with urothelial carcinoma of the upper urinary tract. PMID: 29518027
7. TAB1 was identified as a functional target of miR-134, and the expression of TAB1 was increased by the transcription factors of NF-kappaB1, c-Rel, and ELK1 via miR-134. PMID: 28206956
8. This study indicated that lncrna-TCONS_00026907 was upregulated in cervical cancer and TCONS_00026907 promoted the progression of cervical cancer through inhibition of miR-143-5p and promotion of ELK1. PMID: 28544557
9. FOXE1 interacts with ELK1 on thyroid relevant gene promoters, establishing a new regulatory pathway for its role in adult thyroid function. Co-regulation of TERT suggests a mechanism by which allelic variants in/near FOXE1 are associated with thyroid cancer risk. PMID: 27852061
10. Data suggest that inability of lithium, an anti-manic agent, to regulate circadian rhythms in cells from patient with bipolar disorder reflects reduced ERK1/2 activity and MAP kinase signaling through ELK1. (ERK = extracellular signal-related kinase; ELK1 = ETS-domain protein ELK1) PMID: 27216486
11. we have shown that miR-1275 is a novel negative regulator of human visceral preadipocyte differentiation, which appears to act via post-transcriptional silencing of ELK1. PMID: 27154547
12. Purified ELK1 and AR bound with a dissociation constant of 1.9 x 10(-8) m A purified mutant ELK1 in which the D-box and DEF motifs were disrupted did not bind AR. PMID: 27793987
13. Study identified ELK1 as a novel target for miR- 150 which is up-regulated in apoptotic human umbilical cord vein endothelial cells. PMID: 28110404
14. Study identified a core region encompassing nt - 118 to + 108 of IPO4 gene that is necessary for its promoter activity. Transcription factors binding to this region were screened, resulting in the identification of two members of the Ets family, Ets-like transcription factor-1 and GA binding protein, which repress or activate its promoter activity, respectively. PMID: 28254634
15. High expression of ELK1 is associated with cholangiocarcinoma. PMID: 27658773
16. Overexpression of PAD4 constrains the activity of EMT via suppressing Elk1 expression. PMID: 27176594
17. These data reveal a novel role for Elk1 regulating ITGB6 expression and highlight how dysregulation of Elk1 can contribute to human disease. PMID: 26861876
18. Downregulated expression of transcriptional activator ELK-1 may play an important role in the pathogenesis of atrial fibrillation. PMID: 26617947
19. ELK1 is likely to be activated in prostate cancer cells and promote tumor progression. Furthermore, silodosin that inactivates ELK1 in prostate cancer cells not only inhibits their growth but also enhances the cytotoxic activity of gemcitabine. PMID: 26864615
20. results suggest that ELK1 plays an important role in bladder tumorigenesis and cancer progression. PMID: 26342199
21. Negative feedback regulation of AXL by miR-34a modulates apoptosis in lung cancer cells by activating the transcription factor ELK1 via the JNK signaling pathway. PMID: 26667302
22. These findings suggest that PKCalpha expression in HCC could be stimulated by the formation of MZF-1/Elk-1 complex, which directly binds to the PKCalpha promoter. PMID: 26010542
23. interleukin-1beta (IL-1beta)-induced IER3 expression is mediated by the ERK1/2 target, transcription factor Elk-1. PMID: 25066273
24. Data show that afatinib reduced Elk-1 transcription factor binding to the CIP2A protein promoter and suppressed CIP2A transcription. PMID: 25537503
25. TNF-alpha modulation of intestinal epithelial tight junction barrier is regulated by ERK1/2 activation of Elk-1. PMID: 24121020
26. Analysis implies a role of ELK-1 in the differences between pluripotent stem cells with distinct X chromosome inactivation statuses. PMID: 23871667
27. Elk-1 interacts with the cell cycle kinase Aurora-A, and when Aurora inhibitors are used, P-S383-Elk-1 fails to localize to the poles and remains associated with DNA. PMID: 23322625
28. our data suggest that preferential binding of activated Elk-1 to the IL10 rs3122605-G allele upregulates IL10 expression and confers increased risk for SLE in European Americans. PMID: 24130510
29. Two members of the ETS (E-26) family (PEA3 and ELK-1) regulate the expression of miRNA-200b. PEA3 promotes the expression of miRNA-200b, and ELK-1 is a transcriptional repressor of miRNA-200b. PMID: 24072701
30. FBXO25 mediates ELK-1 degradation through the ubiquitin proteasome system and thereby plays a role in regulating the activation of ELK-1 pathway in response to mitogens PMID: 23940030
31. The binding of Ets1 and Elk1 together to the proximal CIP2A promoter is absolutely required for CIP2A expression in cervical, endometrial and liver carcinoma cell lines PMID: 23117818
32. Sorafenib induces endometrial carcinoma apoptosis by inhibiting Elk-1-dependent Mcl-1 transcription and inducing Akt/GSK3beta-dependent protein degradation. PMID: 23463670
33. Authors define the minimal promoter region of EVI1 and demonstrate that RUNX1 and ELK1, two proteins with essential functions in hematopoiesis, regulate EVI1 in AML. PMID: 22689058
34. Ethanol increases Pol III transcription through a response element which is composed of the overlapping Elk1 and AP-1 binding sites of the TBP promoter. The binding sites may play a role in ethanol-induced deregulation of Pol III genes in liver tumors. PMID: 23454483
35. Strikingly, promoters bound by ELK1 without ERK2 are occupied by Polycomb group proteins that repress genes involved in lineage commitment. PMID: 23727019
36. although ELK1 and GABPA ultimately control the same biological process, they do so by regulating different cohorts of target genes associated with cytoskeletal functions and cell migration contro PMID: 23284628
37. Elk-1 pT417 is present in epithelial cell nuclei of various normal and cancer tissues and the number of pT417-positive cells correlates with differentiation grade of colonic adenocarcinomas. PMID: 23114923
38. The ETS domain transcription factor ELK1 directs a critical component of growth signaling by the androgen receptor in prostate cancer cells. PMID: 23426362
39. Elk1 is positively associated with estrogen receptor and Cyclin D1 expression in breast cancer. Luminal A/B Her-2 negative subtypes showed more Elk-1 activity compared to Her-2 and Basal subtype. No clinicopathologic or prognostic associations were found. PMID: 23127278
40. This is demonstrated for the unique binding mode where a novel role for ELK1 in controlling cell migration is revealed. PMID: 22589737
41. demonstrate that ELK-1 expression arises by a combination of leaky scanning and reinitiation, with the latter mediated by the small upstream ORF2 conserved in both spliced isoforms PMID: 22354998
42. Although the findings showed elevated expression of Elk-1 and PKCalpha in 5637 cells, the regulator of PKCalpha in bladder cancer cells is yet to be determined. PMID: 22559731
43. association between the expression of PKCalpha and the expression of the transcription factors Elk-1 and MZF-1 in breast cancer cell lines PMID: 22242952
44. Activation of the Elk-1 led to an increased survival and a proliferative response with the EGF stimulation, and knocking-down the Elk-1 caused a decrease in survival of U138 glioblastoma cells. PMID: 22085529
45. Formation of ternary complex of human biliverdin reductase-protein kinase Cdelta-ERK2 protein is essential for ERK2-mediated activation of Elk1 protein, nuclear factor-kappaB, and inducible nitric-oxidase synthase (iNOS). PMID: 22065579
46. DJ-1 regulates SOD1 expression through Erk1/2-Elk1 pathway in its protective response to oxidative insult. PMID: 21796667
47. the ERK/ELK-1 cascade is involved in p53-independent induction of p21 and BAX gene expression. PMID: 21642427
48. Genome-wide analysis reveals PADI4 cooperates with Elk-1 to activate c-Fos expression in breast cancer cells. PMID: 21655091
49. AC3-33 is a novel member of the secretory family and inhibits Elk1 transcriptional activity via ERK1/2 MAP PMID: 20680465
50. preferential activation of PTPRZ1 by HIF-2 results at least in part from cooperative binding of HIF-2 and ELK1 to nearby sites on the PTPRZ1 promoter region PMID: 20224786
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亞細胞定位：

Nucleus.
蛋白家族：

ETS family
組織特異性：

Lung and testis.
數據庫鏈接：

HGNC: 3321

OMIM: 311040

KEGG: hsa:2002

STRING: 9606.ENSP00000247161

UniGene: Hs.181128

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