Phospho-EIF2S1 (Ser49) Antibody

說明書

中文名稱：

磷酸化-EIF2S1 (Ser49)兔多克隆抗體
貨號：

CSB-PA279986
規(guī)格：

￥2454
圖片：
Western blot analysis of extracts from Hela cells untreated or treated with starvation using eIF2a(phospho-Ser49) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser49) Antibody (left) or the same antibody preincubated with blocking peptide (right).

Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(phospho-Ser49) Antibody.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2α (Phospho-Ser49) Antibody.
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) EIF2S1 Polyclonal antibody
Uniprot No.：

P05198
基因名：

EIF2S1
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Peptide sequence around phosphorylation site of Serine 49 (L-L-S(p)-E-L) derived from Human eIF2a.
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
濃度：

It differs from different batches. Please contact us to confirm it.
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC,IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:1000
IHC	1:50-1:100
IF	1:100-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S1/eIF-2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming.
基因功能參考文獻：
1. Our study revealed that p-eIF2alpha was upregulated in breast cancer and represented a novel predictor of prognosis in patients with triple-negative subtype. PMID: 28294178
2. hypusine-eIF5A-facilitated translation elongation promotes arsenite-induced polysome disassembly and stress granule assembly in cells subjected to adverse environmental conditions PMID: 20376341
3. analysis of the ER stress response in immunogenic cell death and the potential value of eIF2alpha phosphorylation as a biomarker for this clinically relevant variant of apoptosis [review] PMID: 25749194
4. The data highlight independent interactions of PP1 and eIF2alpha with GADD34, demonstrating that GADD34 functions as a scaffold both in vitro and in cells PMID: 26095357
5. These experiments connect embryonic stem cell growth factors to eIF2alpha phosphorylation. PMID: 26406898
6. We identified EIF2A phosphorylation as a novel early molecular event occurring in response to NAMPT inhibition and mediating protein synthesis arrest. PMID: 26542945
7. The results suggest that dephosphorylation of eIF2a by GADD34 plays an important role in doxorubicin resistance of MCF-7/ADR cells. PMID: 26743901
8. The Newcastle disease virus-induced translation shutoff at late infection times was attributed to sustaining phosphorylation of eIF2a, which is mediated by continual activation of PKR and degradation of PP1. PMID: 26869028
9. GHRH and GHRH-R loops are involved in placental choriocarcinoma cell line viability and apoptosis through Akt and eIF2a pathways. PMID: 26917260
10. These findings suggest that phosphorylated-eIF2alpha regulates synaptic actions of nicotine in both mice and humans, and that reduced phosphorylated-eIF2alpha may enhance susceptibility to nicotine (and other drugs of abuse) during adolescence. PMID: 26928076
11. This review consolidates current information regarding eIF2alpha phosphorylation in neurons and its impact in neurodegenerative diseases. PMID: 26994324
12. Increased phosphorylation of eIF2alpha in chronic myeloid leukemia cells stimulates secretion of matrix modifying enzymes. PMID: 27802179
13. Stress-resistant translation of c-Src mRNA is mediated by eIF2A. PMID: 27899592
14. Results mechanistically link multiple forms of dystonia and put forth a new overall cellular mechanism for dystonia pathogenesis, impairment of eIF2alpha signaling, a pathway known for its roles in cellular stress responses and synaptic plasticity. PMID: 27939583
15. Single amino acid substitution in LC-CDR1 induces Russell body phenotype that attenuates cellular protein synthesis through eIF2alpha phosphorylation and thereby downregulates IgG secretion despite operational secretory pathway traffic. PMID: 28379093
16. These results demonstrate a previously unrecognized role of IL24 in inhibition of translation, mediated through both phosphorylation of eIF2alpha and dephosphorylation of 4E-BP1, and provide the first direct evidence for translation control of gene-specific expression by IL24 PMID: 28461326
17. eukaryotic elongation factor 2 has a role in proliferation and invasion of lung squamous cell carcinoma PMID: 27542262
18. a novel, positive role for PKR activation and eIF2alpha phosphorylation in human globin mRNA splicing, is reported. PMID: 28374749
19. Data show that eIF2Balpha and eIF2Bbeta bind to adjacent surfaces on eIF2alpha-N-terminal domains (NTDs). PMID: 29036434
20. Data show that eIF5-mimic protein (5MP) represses non-AUG translation by competing with translation initiation factor 5 (eIF5) for the Met-tRNAi-binding factor eIF2. PMID: 28981728
21. These data indicate that PERK regulates radioresistance in oropharyngeal carcinoma through NF-kB activation-mediated phosphorylation of eIF2alpha PMID: 28418119
22. The stem-loop of noncoding RNA 886 is structural feature not only critical for inhibiting PKR autophosphorylation, but also the phosphorylation of its cellular substrate, EIF-2alpha. PMID: 28069888
23. overexpression of eIF5 and 5MP induces translation of ATF4. PMID: 27325740
24. Through pathway analysis it was suggested the involvement of eIF2 and mTOR in host cells upon Chlamydia trachomatis infection. PMID: 27134121
25. PERK-eIF2alpha-ATF4 signaling pathway mediated by endoplasmic reticulum stress involved in osteoblast differentiation of periodontal ligament cells under cyclic mechanical force. PMID: 27079961
26. EnR stress assessed by expression of PERK and p-eIF2alpha was significantly associated with tumor infiltrating lymphocytes (TILs) in HER2-positive breast cancer. PMID: 27272779
27. we unravels a new miRs-based mechanism that helps maintain intracellular proteostasis and promote cell survival during ER stress through upregulation of miR-30b-5p and miR-30c-5p which target eIF2alpha and thereby inhibit the p-eIF2alpha/ATF4/CHOP pro-apoptotic pathway, identifying miR-30b-5p and miR-30c-5p as potentially new targets for anti-cancer therapies. PMID: 26898246
28. Our work suggests that OLA1 is a novel translational GTPase and plays a suppressive role in translation and cell survival, as well as cancer growth and progression. PMID: 26283179
29. a delay in eIF2-bound GTP hydrolysis should occur. In this work, we reconstructed this situation and found that such a delay leads to the redistribution of initiation complexes in favor to downstream AUG codons. PMID: 26717981
30. The reactive oxygen species-generating NADPH oxidase-4 (Nox4) is induced downstream of ATF4, binds to a PP1-targeting subunit GADD34 at the endoplasmic reticulum, and inhibits PP1 activity to increase eIF2alpha phosphorylation and ATF4 levels. PMID: 26742780
31. Classical swine fever virus (CSFV) infection increased the phosphorylation of eukaryotic translation initiation factor (eIF)2alpha and its kinase PKR. The activation of PKR during CSFV infection is beneficial to the virus. PMID: 25899421
32. eIF2alpha phosphorylation plays a role in hypoxia-induced translational attenuation PMID: 12370288
33. phosphorylation of eIF2alpha during early brain reperfusion is carried out by PERK, these findings suggest that there is prolonged activation of the unfolded protein response in the reperfused brain. PMID: 12687390
34. multiple domains in I-1 target cellular PP1 complexes, and I-1 has a role as a cellular regulator of eIF2alpha phosphorylation PMID: 15345721
35. Tyrosine phosphorylation acts as a molecular switch to full-scale activation of the eIF2alpha RNA-dependent protein kinase. PMID: 16373505
36. Our results demonstrate that Ebp1 is a new dsRNA-binding protein that acts as a cellular inhibitor of eIF2alpha phosphorylation suggesting that it could be involved in protein translation control PMID: 16631606
37. Double-stranded RNA-dependent protein kinase phosphorylation of the alpha-subunit of eukaryotic translation initiation factor 2 mediates apoptosis PMID: 16717090
38. We propose that SG modeling can occur via both eIF2alpha phosphorylation-dependent and -independent pathways that target translation initiation. PMID: 16870703
39. functional eIF2alpha played an essential role in PS-341-induced Noxa expression PMID: 16928686
40. stress-induced phosphorylation of eIF2 alpha is directly coupled to mitochondrial apoptosis regulation via translational repression of MCL-1 PMID: 17553788
41. the induction of the PKR/eIF2alpha cellular response may be a previously unrecognized general feature of at least the Dependovirus genus of the Parvovirinae PMID: 17715234
42. PKR and PKR-like endoplasmic reticulum kinase induce the proteasome-dependent degradation of cyclin D1 via a mechanism requiring eukaryotic initiation factor 2alpha phosphorylation PMID: 18063576
43. ATF4 contributes to basal ATF5 transcription, and eIF2 kinases direct the translational expression of multiple transcription regulators by a mechanism involving delayed translation reinitiation PMID: 18195013
44. MEK functions to enhance GCN2-dependent eIF2alpha phosphorylation rather than suppressing dephosphorylation PMID: 18287093
45. Proteasomal but not lysosomal inhibitors enhanced GADD34 stability and eukaryotic initiation factor 2alpha (eIF-2alpha) dephosphorylation, a finding consistent with GADD34's role in assembling an eIF-2alpha phosphatase PMID: 18794359
46. GADD34 translation is regulated by a unique 5'UTR uORF mechanism to ensure proper GADD34 expression during eIF2alpha phosphorylation PMID: 19131336
47. Strategies that maintain eIF2alpha in a hyperphosphorylated state may be a novel therapeutic approach to maximize bortezomib-induced apoptosis and reduce residual disease and recurrences in multiple myeloma. PMID: 19190324
48. Unlike other DNA damage response-inducing agents, RITA treatment of cells induced a p53-dependent increase in phosphorylation of the eif2 alpha, requiring PKR-like endoplasmic reticulum kinase activity, and led to the downregulation of HIF-1alpha PMID: 19223463
49. UV-induced eIF2alpha phosphorylation by activation of both PERK and GCN2 via oxidative stress and l-arginine starvation signaling pathways. PMID: 19586904
50. PKR is activated in adenovirus-infected cells with multiple layers of regulation imposed on eIF2alpha phosphorylation by the E1B-55K/E4orf6 complex, which also regulates viral late protein synthesis PMID: 19605483
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亞細胞定位：

Cytoplasm, Stress granule.
蛋白家族：

EIF-2-alpha family
數(shù)據(jù)庫鏈接：

HGNC: 3265

OMIM: 603907

KEGG: hsa:1965

STRING: 9606.ENSP00000256383

UniGene: Hs.151777

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