Phospho-CAMK2A (Thr286) Antibody

說明書

中文名稱：

磷酸化-CAMK2A (Thr286)兔多克隆抗體
貨號：

CSB-PA293488
規格：

￥2454
圖片：
Western blot analysis of extracts from 293 cells untreated or treated with PMA using CaMKII(Phospho-Thr286) Antibody.

Western blot analysis of extracts from Rat brain tissue treated with Lambda Phosphotase or calf intestinal phosphatase (CIP),using CaMKII (Phospho-Thr286) Antibody.
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CAMK2A Polyclonal antibody
Uniprot No.：

Q9UQM7
基因名：

CAMK2A
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Peptide sequence around phosphorylation site of threonine 286 (Q-E-T(p)-V-D) derived from Human CaMKII.
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
濃度：

It differs from different batches. Please contact us to confirm it.
產品提供形式：

Liquid
應用范圍：

ELISA,WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission. Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity. Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity.
基因功能參考文獻：
1. SK current is increased via the enhanced activation of CaMKII in patients with atrial fibrillation. PMID: 29737974
2. Study first demonstrated that CaMKII-alpha was over-expressed in human colon cancers and was associated with cancer differentiation. PMID: 28970726
3. oxidative stress activates the TRPM2-Ca(2+)-CAMK2 cascade to phosphorylate BECN1 resulting in autophagy inhibition PMID: 27245989
4. two peptides (SIAPNV(-COOH) and SIVMNV(-COOH)) were identified to have considerably improved affinity with K d increase by ~tenfold relative to wild type peptide. Thus, the two peptides are considered as promising lead entities to develop therapeutic molecular agents with high efficacy and specificity to target CaMKIIalpha-MUPP1 interaction. PMID: 26984442
5. The importance of CAMK2A and CAMK2B and their auto-phosphorylation in human brain function. PMID: 29100089
6. This study demonstrate that this ASD-linked de novo CAMK2A mutation disrupts multiple CaMKII functions, induces synaptic deficits, and causes ASD-related behavioral alterations. PMID: 28130356
7. CaMKII-mediated recruitment and upregulation of CYLD is expected to remove K63-linked polyubiquitins and facilitate proteasomal degradation at the postsynaptic density. PMID: 24614225
8. CAMK2A SNPs were associated with Alzheimer disease and mild cognitive impairment. AG genotype at the CAMK2A-rs3822606 was associated with AD risk. PMID: 24384746
9. CaMKII phosphorylates SCN5A in vitro on 23 novel serine sites as was identified by mass spectrometry; reduced S516 phosphorylation has been found in human heart failure. PMID: 25815641
10. Ca2+/calmodulin-dependent protein kinase-II (CaMKII) has a key role in the plasticity of glutamatergic synapses of the brain. PMID: 25290264
11. we describe for the first time, two patients with MFD and ID and for whom a deletion encompassing TCOF1 and CAMK2A has been identified PMID: 23695276
12. a novel regulation of CaMKII by another second messenger system and indicate its involvement in excitotoxic neuronal cell death. PMID: 24855644
13. Overexpression of a T253D phosphomimic form of calcium/calmodulin-dependent protein kinase type II subunit alpha significantly decreases proliferation, and cells accumulate in mitosis, specifically in metaphase. PMID: 24407174
14. results suggest that the CAMK2A gene may influence spatial and non-SWM performance in humans without any corresponding gross changes in frontal cortex or hippocampal anatomy. PMID: 22824813
15. found seven significant associations between CAMK2A SNPs and alcohol dependence, one of which in an autophosphorylation-related area of the gene. PMID: 23459588
16. These results suggest that Osterix is a novel target of CaMKII and the activity of Osterix can be modulated by a novel mechanism involving CaMKII during osteoblast differentiation. PMID: 23402759
17. Rem2 has a role in neuronal plasticity hrough co-trafficking with CaMKIIa PMID: 22815963
18. The decrease in CaMKII signaling in the absence of CAPN3 is associated with a reduction of muscle adaptation response. PMID: 22505582
19. The role of CaMKII in regulating GLUT4 expression in skeletal muscle. PMID: 22496345
20. Findigs show that the F-actin-binding protein alpha-actinin-2 targets CaMKIIalpha to F-actin in cells by binding to the CaMKII regulatory domain. PMID: 22427672
21. Inactivating the alphaCamKII transgene eliminates both the IA-type potassium current-mediated firing decrease and the elevated behavioral response to cocaine. PMID: 22573680
22. Kv4.3 K channels contribute to cell apoptosis and necrosis through activating CaMKII. PMID: 22023388
23. Characterization of a central Ca2+/calmodulin-dependent protein kinase IIalpha/beta binding domain in densin that selectively modulates glutamate receptor subunit phosphorylation. PMID: 21610080
24. 20 single nucleotide polymorphisms had suggestive associations with conduct disorder, nine of which were located in known genes, including CAMK2A PMID: 21611732
25. Mice heterozygous for a null mutation of the alpha-isoform of calcium/calmodulin-dependent protein kinase II (alpha-CaMKII+/-) have profoundly dysregulated behaviours and impaired neuronal development in the dentate gyrus PMID: 18803808
26. Knockdown of spinal CaMKIIalpha attentuates opioid-induced hyperalgesia. PMID: 20053885
27. Four distinct isoforms of CAMKII were isolated. Two of them were characterized as CaMKII alpha and beta subunits.CaMKII expression is developmentally regulated in human fetal and adult brain PMID: 11710563
28. the NPY Y(1) receptor induces the expression of CRE containing target genes through the CaM kinase-CREB pathway PMID: 11814622
29. role in cell communication PMID: 11889801
30. CaMKII alpha mRNA expression is significantly reduced in the prefrontal cortex of patients with bipolar illness. PMID: 11930170
31. Calcium/calmodulin-dependent protein kinase II binds to Raf-1 and modulates integrin-stimulated ERK activation PMID: 12954639
32. measured differences in CaMKII binding affinities for CaM play a minor role in the autophosphorylation of the enzyme, largely dictated by autophosphorylation rate for alpha, beta, gamma and delta isoforms PMID: 14722083
33. CaMKII-alpha may be related with beta-amyloid more closely than being involved in tau hyperphosphorylation in Alzheimer's disease PMID: 15621017
34. novel mechanism for Ca2+-dependent negative-feedback regulation of NR2B-containing NMDARs in a CaMKII activity- and autophosphorylation-dependent manner that may modulate NMDAR-mediated synaptic plasticity PMID: 15866054
35. activation of the IKK/NFkappaB signaling cascade by SSTR2 requires a complicated network consisting of Galpha(14), protein kinase C, CamkII, ERK, and c-Src PMID: 16115892
36. EGF has the ability to abrogate the PP2A function in the maintenance of beta1 integrin-mediated cell adhesion by dissociation of PP2A-IQGAP1-CaMKII from beta1 integrin-Rac through activation of CaMKII PMID: 16557530
37. CD44 interaction with LARG and EGFR plays a pivotal role in Rho/Ras co-activation, PLC epsilon-Ca2+ signaling, and Raf/ERK up-regulation required for CaMKII-mediated cytoskeleton function and in head and neck squamous cell carcinoma progression PMID: 16565089
38. To characterize the human alphaCaMKII promoter, a promoter-reporter gene assay using different cell lines, was developed. PMID: 17221287
39. Skeletal muscle CaMKII kinase isoform expression and serum response factor phosphorylation is higher with endurance-type exercise training, adaptations that are restricted to active muscle. PMID: 17627985
40. alpha-CaMKII controls the growth of human osteosarcoma by regulating cell cycle progression PMID: 17632540
41. These findings revealed that TNF-alpha induced VCAM-1 expression via multiple signaling pathways. PMID: 18227124
42. hCaMKIINalpha suppresses tumor growth by inducing cell cycle arrest via p27 stabilization PMID: 18305109
43. Phosphorylation or a phosphorylation mimicking mutation on NR2B (NR2B-S1303D) abolishes the Ca(2+)/calmodulin-independent binding whereas it allows the Ca(2+)/calmodulin-dependent binding of alpha-CaMKII in vitro. PMID: 19453375
44. Data show that data provide a novel mechanism whereby CaMKII may regulate the proteasome in neurons to facilitate remodeling of synaptic connections through protein degradation. PMID: 19638347
45. Amphetamine sensitization in rats, an animal model of schizophrenia, results in significant increase in CaMKII beta and a nonsignificant increase in CaMKII alpha mRNA. PMID: 17603807
46. The expression of CaMKIIalpha is significantly elevated in depression (29%), but not in schizophrenia or bipolar disorder, compared to healthy controls. PMID: 16247765
47. The expression of CaMKII alpha was significantly elevated in depression. PMID: 16247765
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亞細胞定位：

Cell junction, synapse. Cell junction, synapse, postsynaptic density. Cell projection, dendritic spine. Cell projection, dendrite.
蛋白家族：

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
數據庫鏈接：

HGNC: 1460

OMIM: 114078

KEGG: hsa:815

STRING: 9606.ENSP00000381412

UniGene: Hs.743976

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