PRKG1 Antibody

說明書

中文名稱：

PRKG1兔多克隆抗體
貨號：

CSB-PA619797ESR2HU
規格：

￥440
圖片：
Western blot
All lanes: cGMP-dependent protein kinase 1 antibody at 3μg/ml + Mouse lung tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 77, 78, 45 kDa
Observed band size: 77 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) PRKG1 Polyclonal antibody
Uniprot No.：

Q13976
基因名：

PRKG1
別名：

cGK 1 antibody; cGK1 antibody; cGKI alpha antibody; cGKI antibody; cGKI beta antibody; cGMP-dependent protein kinase 1 antibody; cGMP-dependent protein kinase I antibody; DKFZp686K042 antibody; FLJ36117 antibody; KGP1_HUMAN antibody; KGPB antibody; MGC71944 antibody; pkg antibody; PRKG1 antibody; PRKG1B antibody; PRKGR1A antibody; PRKGR1B antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Recombinant Human cGMP-dependent protein kinase 1 protein (1-300AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates IRAG1 and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.
基因功能參考文獻：
1. our study provides the first genome-wide evidence regarding a mechanism by which traumatic life events interact with human genetic variation of PRKG1 in relation to alcohol misuse PMID: 28265120
2. Disulfide-activated PKGIalpha regulates cardiac diastolic relaxation and fine-tunes the Frank-Starling response. PMID: 27782102
3. PRKG1, known to induce apoptosis when activated, is increased in all patient derived glioblastoma spheres PMID: 27564115
4. redox-mediated guidance required PKG1 and consequently, its deficiency in vivo resulted in defective repair and enhanced neuropathic pain after nerve injury. PKG1-dependent repair functions will outweigh its signaling functions in spinal nociceptive LTP, so that inhibition of PKG1 is no option for neuropathic pain. PMID: 27978504
5. Oxidant signaling underlies PKGIalpha modulation of Ca2+ sparks and BKCa in myogenically active arterioles PMID: 27729549
6. These affinity and activation measurements show that beta-phenyl-1,N2-ethenoguanosine 3',5'-monophosphate (PET-cGMP) is most selective for PKG I, whereas 8-(4-chlorophenylthio)guanosine-3'-5'-cyclic monophosphate (8-pCPT-cGMP) is most selective for PKG II. PMID: 28793191
7. Hence, SO2 exerted its vasodilatory effects at least partly by promoting disulfide-dependent dimerization of sGC and PKG, resulting in an activated sGC/cGMP/PKG pathway in blood vessels. These findings revealed a new mode of action and mechanisms by which SO2 regulated the vascular tone. PMID: 27009048
8. these data indicate PKG as an intermediary in EGFR-mediated cell death, likely via apoptotic pathway. PMID: 27381222
9. The heterozygous c.530G>A:p.Arg177Gln PRKG1 variant was associated with early-onset thoracic aortic aneurysm and dissection in a Spanish family. PMID: 27442293
10. cAMP is a partial agonist for PKG, and we elucidate the mechanism for cAMP partial agonism through the comparative NMR analysis of the apo, cGMP-, and cAMP-bound forms of the PKG cyclic nucleotide-binding domain B. PMID: 26370085
11. Protein kinase G has a role in regulating production of H2S, which governs oxygen sensing PMID: 25900831
12. The C42-C42' disulfide bond dramatically stabilizes PRKG1. PMID: 26132214
13. The Golgi apparatus regulates PKGIalpha compartmentation and proteolysis. PMID: 25855081
14. Study of genetic heterogeneity in systemic lupus erythematosus, the top associations in European ancestry were protein kinase, cyclic GMP-dependent, type I (PRKG1) rs7897633 (P(Meta) = 2.75 x 10(-8)) and purine nucleoside phosphorylase (PNP) rs1049564 (P(Meta) = 1.24 x 10(-7)). PMID: 25338677
15. Expression levels of miR-517a-3p and its target mRNA PRKG1 were inversely correlated in NK cells before and after obstetric delivery. PMID: 25273530
16. miR-20a expression is up-regulated in response to hypoxia in both mouse and human pulmonary arterial smooth muscle cells PMID: 25447536
17. Data show that functional cGMP/protein kinase G type 1 (PKG1) is expressed in pancreatic adenocarcinoma cells. PMID: 24826884
18. These findings characterize PKG as a novel regulator of AR-mediated transcription by enhancing AR cofactor p44/WDR77's function. PMID: 23755100
19. Neutron diffraction reveals hydrogen bonds critical for cGMP-selective activation: insights for cGMP-dependent protein kinase agonist design. PMID: 25271401
20. Leucine296 and Arginine297 are key residues of PKG I that mediate cGMP selectivity. PMID: 24239458
21. These data suggest that increased PKG II activity attenuates bFGFinduced proliferation and migration by inhibiting the MAPK/ERK signaling pathway, whereas PKG I does not PMID: 23404188
22. nitration of Tyr(247) and the attenuation of cGMP binding is an important mechanism regulating in PKG-1alpha activity and SMC proliferation/differentiation PMID: 24469460
23. Authors show for the first time that PKG is also able to stably interact with a viral substrate, West Nile virus NS5, in cell culture and in vitro. PMID: 23876037
24. These findings indicate that PRKG1 risk alleles associate with salt-sensitivity related to a loss of the inhibitory control of renal Na(+) reabsorption, suggestive of a blunt pressure-natriuresis response. PMID: 24060892
25. left ventricular systolic radial function was associated with common polymorphisms in PRKG1. PMID: 24060898
26. Recurrent gain-of-function mutation in PRKG1 causes thoracic aortic aneurysms and acute aortic dissections. PMID: 23910461
27. activation of PKGI with cGMP regulated SSG1 intracellular distribution. PMID: 23831687
28. ANP attenuates the inflammatory actions of histamine via endothelial GC-A/cGMP/cGKI signaling and inhibitory phosphorylation of TRPC6 channels. PMID: 23814119
29. The data suggest that PKGIbeta enhances breast cancer cell motility and invasive capacity, at least in part, by phosphorylating CaD. PMID: 23418348
30. PKG positively regulates proteasome activities and proteasome-mediated degradation of misfolded proteins, likely through posttranslational modifications to proteasome subunits. PMID: 23770744
31. overexpression of PCs, furin and PC5, but not PC7, which are all expressed in SMC, increase PKGI cleavage in a dose-dependent manner PMID: 23686857
32. The cyclic GMP-dependent kinase Ialpha -induced RhoA activation is dependent on the activity of ROCK and serine/threonine phosphatases. PMID: 23195829
33. Studies indicate the presence of the switch helix (SW), a novel motif that promotes the formation of a domain-swapped dimer in the asymmetric unit. PMID: 23416533
34. Low myocardial PKG activity in heart failure with preserved ejection fraction was associated with raised cardiomyocyte F(passive) and was related to increased myocardial nitrosative/oxidative stress. PMID: 22806632
35. RhoA is a PKGIalpha target and direct binding of activated PKGIalpha to RhoA is central to cGMP-mediated inhibition of the VSMC Rho kinase contractile pathway PMID: 23066013
36. Downregulation of PKG1 was associated with malignant and benign breast tumors. PMID: 22791569
37. These results suggest that DAPK2 is one of the targets of cGK-I in apoptosis induction. PMID: 22580283
38. PKGIbeta inhibited cell growth and inducd apoptosis in breast cancer cells. PMID: 22095901
39. cGMP-dependent protein kinase (PKG) Ialpha transfection significantly attenuated the hypoxia-induced down-regulation of the expressions of SM-alpha-actin, MHC and calponin. PMID: 22293199
40. found in smooth muscle wall of blood vessels transversing the clitoral supepithelial and stromal space PMID: 21697861
41. Cyclic 3',5'-guanosine monophosphate-dependent protein kinase inhibits colon cancer cell adaptation to hypoxia. PMID: 21563174
42. cGMP dependent kinase 1 alpha interacts with and phosphorylates transient receptor potential cation channel subfamily C member 7 PMID: 21402151
43. data suggest that activation of PKG signaling in pericytes inhibits focal adhesion formation by inhibiting Rac1 PMID: 21451103
44. NO, depending on its concentration, may act in human PBMCs as a stimulator of IL-6 expression and modulator of NF-KB involving the sGC/cGMP/PKG pathway PMID: 21414799
45. The mechanism of cyclic nucleotide binding to PKG was investigated by determining crystal structures of the amino-terminal cyclic nucleotide-binding domain (CNBD-A) of human PKG I bound to either cGMP or cAMP. PMID: 21526164
46. The novel cGMP/PKG/ROS/calmodulin/CaMKII signaling pathway may regulate cardiomyocyte excitability by opening K(ATP) channels and contribute to cardiac protection against ischemia-reperfusion injury. PMID: 21479273
47. These findings provide evidence of a physical and compartmentalized association between SERT and PKGIalpha that supports. PMID: 19656393
48. A novel mechanism has been elucidated that is able to increase PKG-I protein content in contractile cells, with hypotensive consequences in rat thoracic aorta. PMID: 21160032
49. disruption of the IP3R1-IRAG calcium regulation system is a novel cause of dysfunctional osteoclasts unrelated to defects in attachment proteins or acid secretion PMID: 20567233
50. structure provides molecular details of this unique leucine/isoleucine zipper, revealing specific hydrophobic and ionic interactions that mediate dimerization and demonstrating the topology of the GKAP interaction surface. PMID: 20826808
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相關疾?。?/div>
Aortic aneurysm, familial thoracic 8 (AAT8)
亞細胞定位：

Cytoplasm.
蛋白家族：

Protein kinase superfamily, AGC Ser/Thr protein kinase family, cGMP subfamily
組織特異性：

Primarily expressed in lung and placenta.
數據庫鏈接：

HGNC: 9414

OMIM: 176894

KEGG: hsa:5592

UniGene: Hs.407535

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