PRKACA Antibody
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中文名稱(chēng)：PRKACA兔多克隆抗體
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貨號(hào)：CSB-PA235804
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規(guī)格：￥2024
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圖片：
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Western blot analysis of extracts from Jurkat cells and COLO cells, using KAPC A/B antibody.
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱(chēng)：Rabbit anti-Homo sapiens (Human) PRKACA Polyclonal antibody
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Uniprot No.：
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基因名：
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宿主：Rabbit
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反應(yīng)種屬：Human,Mouse,Rat
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免疫原：Synthesized peptide derived from N-terminal of Human KAPC A/B.
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免疫原種屬：Homo sapiens (Human)
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克隆類(lèi)型：Polyclonal
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純化方式：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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濃度：It differs from different batches. Please contact us to confirm it.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA,WB
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:3000 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, SOX9 and VASP. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in chondrogenesis by mediating phosphorylation of SOX9. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock.; Phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation.
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基因功能參考文獻(xiàn)：
- Ezrin-anchored PKA phosphorylates serine 369 and 373 on connexin 43 to enhance gap junction assembly, communication, and cell fusion. PMID: 29259079
- CaV1.4 channels are indeed modulated by PKA phosphorylation within the inhibitor of Ca(2+)-dependent inactivation (ICDI) motif. PMID: 27456671
- In this study, the authors linked for the first time the loss of RIIbeta protein levels to the PRKACA mutation status and found the down-regulation of RIIbeta to arise post-transcriptionally. PMID: 28250426
- the presence of lipofuscin in cortisol-producing adenomas (CPAs) responsible for Cushing syndrome with and without the PRKACA (pLeu206Arg) somatic mutation, was investigated. PMID: 28834963
- Mechanistically, Sirt1 expression elevates phosphorylation of the alpha subunit of protein kinase A (PKA alpha), and this event is essential for Sirt1-induced phosphorylation of beta Catenin. PMID: 28583374
- PKA, under the conditions of experimental approach appears to function as a master upstream kinase that is sufficient to initiate the complex pattern of intracellular signaling pathway and gene expression profiles that accompany ovarian granulosa cells differentiation PMID: 27324437
- CTR activates AKAP2-anchored cAMP-dependent protein kinase A, which then phosphorylates tight junction proteins ZO-1 and claudin 3. PMID: 28428082
- These results indicate that Mixed fibrolamellar hepatocellular carcinoma (mFL-HCC) is similar to pure FL-HCC at the genomic level and the DNAJB1:PRKACA fusion can be used as a diagnostic tool for both pure and mFL-HCC PMID: 27029710
- PRKACA mutations are highly specific for cortisol over-secretion, while they are absent or very rare in the context of other adrenal diseases. Patients carrying these somatic mutations are affected by a more severe phenotype and are identified at a younger age. PMID: 27871112
- Somatic mutations in PRKACA, coding for the catalytic alpha subunit of protein kinase A (PKA), have been recently identified as the most frequent genetic alteration in cortisol-secreting adrenocortical adenomas, which are responsible for adrenal Cushing's syndrome. PMID: 27813054
- HIF1a transcriptional activity is stimulated by Protein kinase A-dependent phosphorylation PMID: 27245613
- cigarette smoke extracts activate the PKA, CREB, and IL-13Ralpha2 axis in lung endothelial cells. PMID: 27986643
- Data indicate a subpopulation of the CaV1.2 channel pore-forming subunit (alpha1C) within nanometer proximity of protein kinase A (PKA) at the sarcolemma of murine and human arterial myocytes. PMID: 28119464
- we propose that the PKA-Smurf1-PIPKIgamma pathway has an important role in pulmonary tumorigenesis and imposes substantial clinical impact on development of novel diagnostic markers and therapeutic targets for lung cancer treatment. PMID: 28581524
- cAMP/PKA signaling attenuated respiratory syncytial virus-induced disruption of structure and functions of the model airway epithelial barrier by mechanisms involving the stabilization of epithelial junctions and inhibition of viral biogenesis PMID: 28759570
- The mutated PRKACA proteins lost their ability to bind to PRKAR1A, and thereby lead to constitutive activation of the PKA pathway. Together with previous reports of PRKAR1A mutations in syndromic cardiac myxoma, our study demonstrates the importance of the PKA pathway in the tumourigenesis of cardiac myxoma. PMID: 28369983
- differential regulation of PKA and cell stiffness in unconfined versus confined cells is abrogated by dual, but not individual, inhibition of Piezo1 and myosin II. PMID: 27160899
- Data show that the adenylate cyclase (AC) pathway as genes relating to the antitumor activity of xanthohumol (XN) against tumor cells, and the pathway regulates various cellular functions via activating protein kinase A (PKA)-dependent phosphorylation. PMID: 28122154
- PRKACA mutations are present in cortisol-producing adenomas and bilateral adrenal macronodular hyperplasia. PRKACA mutation is associated with more severe autonomous cortisol secretion. PMID: 27296931
- Dual co-expression of human fetal Tau with PKA in Escherichia coli results in multisite Tau phosphorylation including also naturally occurring sites which were not previously considered in the context of 14-3-3 binding. Tau protein co-expressed with PKA displays tight functional interaction with 14-3-3 isoforms of a different type. PMID: 28575131
- This review will discuss the effects of PKA phosphorylation on wild-type CFTR, the consequences of cystic fibrosis mutations on PKA phosphorylation, and the development of therapies that target PKA-mediated signaling. [review] PMID: 27722768
- This interaction is promoted by EPAC1 activation, triggering its translocation to the plasma membrane and binding to NHERF1. Our findings identify a new CFTR-interacting protein and demonstrate that cAMP activates CFTR through two different but complementary pathways - the well-known PKA-dependent channel gating pathway and a new mechanism regulating endocytosis that involves EPAC1. PMID: 27206858
- The inhibitory effects of BMP4 on PDGF-induced cell proliferation, collagen synthesis, and calpain-2 activation are impaired in pulmonary artery smooth muscle cells from pulmonary arterial hypertension patient. PMID: 28235949
- immunohistochemical staining for StAR is a reliable pathological approach for the diagnosis and classification of adrenocortical adenomas with cAMP/PKA signaling-activating mutations. PMID: 27606678
- description of PRKACA mutations in 2 cases of primary aldosteronism (PA) patients: a novel variant (p.His88Asp) in a case of sudden onset of PA and a mutation (p.Leu206Arg) in context of hypokalemic aggravation of long-term hypertension; these genetic alterations were not found in a subsequent series of 120 aldosterone-producing adenomas and thereby appear to be very rare events PMID: 27270477
- PKA signaling is pivotal in pigmentation process itself in cultured melanoma cells, while the importance of Wnt/beta-catenin signaling should be emphasized in the context of development and differentiation PMID: 27567978
- Studies indicate a functional interaction between leucine-rich repeat kinase 2 (LRRK2) and protein kinase A (PKA) cross-talk in neuron and microglia. PMID: 28202680
- Studies indicate the 3'-5'-Cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) signalling pathway at distinct mitochondria subdomains represented by the outer and inner mitochondrial membranes. PMID: 28202681
- CKbeta phosphorylation by MCF-7 cell lysate was inhibited by a PKA-specific inhibitor peptide, and the intracellular phosphorylation of CKbeta was shown to be regulated by the level of cyclic adenosine monophosphate (cAMP), a PKA activator PMID: 27149373
- GPER enhances melanogenesis via PKA by upregulating microphthalmia-related transcription factor-tyrosinase in melanoma PMID: 27378491
- testosterone rapidly increased whole-cell HCAEC SKCa and BKCa currents via a surface androgen receptor, Gi/o protein, and protein kinase A PMID: 28223151
- PRKACA mutation is associated with Adrenocortical Adenomas. PMID: 27389594
- Differentiation of mesenchymal stem cells into beige/brown adipocytes can be related via PKA modulation. PMID: 27498007
- FL-HCCs in children and young adults uniquely overexpress DNAJB1-PRKACA, which results in elevated cAMP-dependent PKA activity PMID: 27027723
- high-resolution models for PKA(WT) and PKA(L)(205R) substrate specificity. PMID: 28100013
- CFTR inhibition also affected cAMP/PKA-downstream events such as the increase in tyrosine phosphorylation, hyperactivated motility, and acrosome reaction. PMID: 27714810
- Results suggest that G protein alpha S subunit (Galphas) plays a tumor-promoting role in renal cell carcinoma (RCC) and possibly acts through a protein kinase A (PKA)-dependent pathway. PMID: 28051330
- Phosphorylation of ezrin together with its binding to phosphatidylinositol-4,5-bisphosphate tethers the F508del CFTR to the actin cytoskeleton, stabilizing it on the apical membrane and rescuing the sub-membrane compartmentalization of cAMP and activated PKA. PMID: 26823603
- Data show that activated PKA phosphorylates the 19S subunit, Rpn6/PSMD11 (regulatory particle non-ATPase 6/proteasome subunit D11) at Ser14. PMID: 26669444
- Our results reveal for the first time the role of the versatile PKA in cancer cells survival under chronic glucose starvation and anoikis and may be a novel potential target for cancer treatment. PMID: 26978032
- PKA phosphorylates the ATPase inhibitory factor 1 and inactivates its capacity to bind and inhibit the mitochondrial H(+)-ATP synthase. PMID: 26387949
- DNAJB1-PRKACA was evaluated as a key driver of fibrolamellar carcinoma and as a candidate therapeutic target PMID: 26505878
- No somatic mutations were observed in PRKACA in a cohort of GH-secreting pituitary adenomas. PMID: 26701869
- Protein kinase A activity is necessary for fission and fusion of Golgi to endoplasmic reticulum retrograde tubules. PMID: 26258546
- Kapbeta2 interacts with ULK2 through ULK2's putative PY-NLS motif, and facilitates transport from the cytoplasm to the nucleus, depending on its Ser1027 residue phosphorylation by PKA, thereby reducing autophagic activity. PMID: 26052940
- ITM2A expression is positively regulated by PKA-CREB signaling and ITM2A expression interferes with autophagic flux by interacting with vacuolar ATPase. PMID: 25951193
- Akt and PKA phosphorylated KLHL3 at S433, and phosphorylation of KLHL3 by PKA inhibited WNK4 degradation. PMID: 26435498
- Data indicate that protein kinase A (PKA) phosphorylates only serine-253 amino acid on activator of G-protein signaling 1 protein Dexras1 (RASD1). PMID: 26358293
- PKA compartmentalization via AKAP220 and AKAP12 contributes to endothelial barrier regulation. PMID: 25188285
- Leu206Arg and Leu199_Cys200insTrp mutations in PRKACA impair its association with PRKAR2B and PRKAR1A. PMID: 25477193
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相關(guān)疾病：Primary pigmented nodular adrenocortical disease 4 (PPNAD4)
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亞細(xì)胞定位：Cytoplasm. Cell membrane. Nucleus. Mitochondrion. Membrane; Lipid-anchor.; [Isoform 2]: Cell projection, cilium, flagellum. Cytoplasmic vesicle, secretory vesicle, acrosome.
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蛋白家族：Protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily
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組織特異性：Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.
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