POU4F1 Antibody

中文名稱：

POU4F1兔多克隆抗體
貨號(hào)：

CSB-PA22749A0Rb
規(guī)格：

￥440
圖片：
Immunofluorescence staining of Hela cells with CSB-PA22749A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate
All lanes: POU4F1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 34 kDa
Observed band size: 43 kDa
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) POU4F1 Polyclonal antibody
Uniprot No.：

Q01851
基因名：

POU4F1
別名：

Brain specific homeobox/POU domain protein 3A antibody; Brain-3A antibody; Brain-specific homeobox/POU domain protein 3A antibody; Brn 3.0 antibody; BRN 3A antibody; Brn-3A antibody; Brn3 antibody; BRN3A antibody; class 4 antibody; FLJ13449 antibody; Homeobox/POU domain protein RDC 1 antibody; Homeobox/POU domain protein RDC-1 antibody; Homeobox/POU domain protein RDC1 antibody; Oct T1 antibody; Oct-T1 antibody; OctT1 antibody; PO4F1_HUMAN antibody; POU class 4 homeobox 1 antibody; POU domain antibody; POU Domain Class 4 Transcription Factor 1 antibody; Pou4f1 antibody; RDC 1 antibody; RDC1 antibody; transcription factor 1 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human POU domain, class 4, transcription factor 1 protein (258-419AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，POU4F1 Antibody (CSB-PA22749A0Rb)，的標(biāo)記方式是Non-conjugated。對(duì)于POU4F1 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA22749B0Rb	POU4F1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA22749C0Rb	POU4F1 Antibody, FITC conjugated
Biotin	CSB-PA22749D0Rb	POU4F1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain). Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site.; Able to act as transcription factor, cannot regulate the expression of the same subset of genes than isoform 1. Does not have antiapoptotic effect on neuronal cells.
基因功能參考文獻(xiàn)：
1. We report a case of a nine-year-old male who presented with facial nerve stimulation four years after cochlear implantation. A dilated internal auditory meatus was revealed. Genetic analysis demonstrated X-linked deafness type 2 (DFNX2) caused by a novel c.769C T nucleotide change in the POU domain, class 3, transcription factor 4 gene PMID: 27863625
2. Brn3a cooperates with activated RAS/RAF signalling by reducing oncogene-induced senescence in melanocytic tumourigenesis. PMID: 23666755
3. Data indicate that median methylation levels of BCAN, HOXD1, KCTD8, KLF11, NXPH1, POU4F1, SIM1, and TCF7L1 were >/=30% higher than in normal samples, representing potential biomarkers for tumor diagnosis. PMID: 22930747
4. PoU4F1 is highly expressed in t(8;21) samples, with AML/ETO appearning to promote some BRN3A expression PMID: 22064348
5. BRN3A possesses anti-apoptotic property, and considering the above results, it may be regarded as the key component in promoting tumorigenic growth in the uterine cervical cells. PMID: 21928122
6. Ewing sarcoma induce expression of neuronal markers such as BRN3A showing that the function of those same markers may be restricted or controlled in an sarcoma-dependent manner. PMID: 20348952
7. Dysregulation of POU4F1 is associated with t(8;21) acute myeloid leukemia. PMID: 20376082
8. genomic organization of the Brn-3a locus and the mechanisms that control the expression of two different proteins from one genomic locus PMID: 12427558
9. Measurement of Brn-3a levels in smears can be used to detect a significant proportion of cervical lesions that were missed by Pap smear. PMID: 12893201
10. These results indicate that Brn-3a could play an important role in the near future in improving cervical cancer screening. PMID: 12911730
11. oncogenic rearrangement of EWS to produce EWS/Fli-1 may enhance the antiapoptotic effect of Brn-3a and inhibit its ability to promote neuronal differentiation. PMID: 15021903
12. Hsp27 expression and cell survival are regulated by the POU transcription factor Brn3a PMID: 15272315
13. Brn-3a has a role in differential regulation of different human papilloma virus variants PMID: 16247485
14. Upregulation of Brn-3a is associated with prostate cancer PMID: 16276351
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亞細(xì)胞定位：

Nucleus. Cytoplasm.
蛋白家族：

POU transcription factor family, Class-4 subfamily
組織特異性：

Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 9218

OMIM: 601632

KEGG: hsa:5457

STRING: 9606.ENSP00000366413

UniGene: Hs.654522

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