Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate
All lanes: POLDIP3 antibody at 3.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 43 kDa
Observed band size: 43 kDa
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA871610LA01HU at dilution of 1:100
Immunofluorescence staining of Hela cells with CSB-PA871610LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating POLDIP3 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA871610LA01HU in Jurkat whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA871610LA01HU (8μg) + Jurkat whole cell lysate (500μg)
Lane 3: Jurkat whole cell lysate (10μg)
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期:
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by association with the TREX complex.
基因功能參考文獻:
Altered expression of PDIP46 or its mutation may affect Pol delta functions in vivo, and thereby be a nexus for altered genomic stability. PMID: 26819372
hnRNP-U inhibits TDP-43-mediated alterations in splicing of POLDIP3 mRNA. PMID: 25378556
PDIP3 and ZC11A associate with the human TREX complex in an ATP-dependent manner and function in mRNA export. PMID: 22928037
these findings establish critical and essential roles for SKAR in the regulation of mRNA translation of IFN-sensitive genes and induction of IFN-alpha biological responses. PMID: 25049393
Alteration of POLDIP3 splicing associated with loss of function of TDP-43 in tissues affected with amyotrophic lateral sclerosis (ALS) . PMID: 22900096
SKAR is a specific target of S6K1 and regulates cell growth. PMID: 15341740
This study showed that PDIP46 aka SKAR, a protein possessing one RNA recognition motif (RRM) and being a protein partner of both the p50 subunit of DNA polymerase delta and p70 ribosomal protein S6 kinase 1 (S6K1). PMID: 16984396
SKAR-mediated recruitment of activated S6K1 to newly processed mRNPs serves as a conduit between mTOR checkpoint signaling and the pioneer round of translation when cells exist in conditions supportive of protein synthesis. PMID: 18423201