POLA1 Antibody
-
中文名稱：POLA1兔多克隆抗體
-
貨號：CSB-PA002170
-
規(guī)格：￥1090
-
圖片：
-
Western Blot analysis of HepG2 cells using DNA pol α Polyclonal Antibody
-
-
其他：
產(chǎn)品詳情
-
Uniprot No.：
-
基因名：
-
別名：DKFZp686K1672 antibody; DNA polymerase alpha 1 catalytic subunit antibody; DNA polymerase alpha catalytic subunit antibody; DNA polymerase alpha catalytic subunit p180 antibody; DNA polymerase alpha p180 subunit antibody; DPOLA_HUMAN antibody; OTTHUMP00000023089 antibody; OTTHUMP00000023090 antibody; P180 antibody; p180 subunit antibody; POLA antibody; POLA1 antibody; Polymerase (DNA directed), alpha 1, catalytic subunit antibody; Polymerase (DNA-directed), alpha (70kD) antibody
-
宿主：Rabbit
-
反應(yīng)種屬：Human
-
免疫原：Synthesized peptide derived from the N-terminal region of Human DNA pol α.
-
免疫原種屬：Homo sapiens (Human)
-
標(biāo)記方式：Non-conjugated
-
抗體亞型：IgG
-
純化方式：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-
濃度：It differs from different batches. Please contact us to confirm it.
-
保存緩沖液：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
-
產(chǎn)品提供形式：Liquid
-
應(yīng)用范圍：WB, ELISA
-
推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 ELISA 1:20000 -
Protocols：
-
儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
-
貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
-
用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻(xiàn)
- Enhanced Anti-Cancer Effects of Conditioned Medium from Hypoxic Human Adult Dermal Fibroblasts on Cervical Cancer Cells KH Han,International journal of molecular sciences,2022
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
-
功能：Catalytic subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, a regulatory subunit POLA2 and two primase subunits PRIM1 and PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses.
-
基因功能參考文獻(xiàn)：
- High POLA1 expression is associated with bladder cancer. PMID: 28320388
- Divalent ions attenuate DNA synthesis by human DNA polymerase alpha by changing the structure of the template/primer or by perturbing the polymerase reaction. PMID: 27235627
- We propose that completely skewed XCI favoring the normal X chromosome resulted from death of cells with an active derivative X that was caused by a non-functional POLA1 gene. In summary, we conclude that functional monosomy of 6q27-qter and functional disomy of Xpter-p22.11 are responsible for the clinical phenotype of the patient PMID: 28371302
- The first crystal structure of human Polalpha polymerase subunit in complex with a DNA:DNA helix shows that portion of the DNA:DNA helix in contact with the polymerase is not in a B-form but in a hybrid A-B form. The free energy cost of distorting DNA from B- to hybrid A-B form may augur the termination of primer synthesis. PMID: 27032819
- Data indicate that X-linked reticulate pigmentary disorder (XLPDR) is caused by an intronic mutation that disrupts the expression of POLA1, which encodes the catalytic subunit of DNA polymerase-alpha. PMID: 27019227
- Mutation in POLA1 is responsible for XLPDR (MIM: 312040) and demonstrates a role for this gene in interferon regulation PMID: 27019227
- Inhibition of DNA polymerases a, delra and e by AFP promoter-driven artificial microRNAs may lead to effective growth arrest of AFP-positive HCC cells,as novel strategy for gene therapy PMID: 25924900
- To understand the regulatory mechanisms and to reveal the details of DNA polymerase alpha organization, the study determined the crystal structure of p70 in complex with C terminus of the POLA catalytic subunit (p180C). PMID: 25847248
- the N-terminal domain of the large subunit of primase (p58N) directly interacts with the C-terminal domain of the catalytic subunit of polalpha (p180C) PMID: 24962573
- The Pol alpha-primase complex. PMID: 22918585
- Pol epsilon and Pol alpha/delta seem to pursue their functions at least in part independently in late S phase PMID: 22887995
- Findings indicate that tethering of primase to the replisome by DNA polymerase alpha (pol alpha) is critical for the normal action of DNA replication forks in eukaryotic cells. PMID: 22593576
- Depletion of p180 in U2OS cells increases cell size. PMID: 22679391
- Human cell DNA replication is mediated by a discrete multiprotein complex. The peak of DNA polymerase alpha activity co-purifies with the peak of in vitro SV40 DNA replication activity. PMID: 11968016
- correlation between binding of CDP/Cux to the DNA pol alpha promoter and the stimulation of gene expression PMID: 12665598
- Data show that human DNA polymerase alpha and the Klenow fragment of Escherichia coli DNA polymerase I (KF) incorporate all four nucleotide analogues opposite all four canonical bases up to 4000-fold more efficiently than incorrect natural bases. PMID: 12950174
- Three-dimensional structures of the zinc finger motif in the carboxy terminus of the human DNA polymerase-alpha were determined in this study. PMID: 14499601
- nonphosphorylated p68 inhibited the stimulation of pol-alpha activity by hyperphosphorylated retinoblastoma protein, suggesting that p68 might impede the association of ppRb with p180 PMID: 16935576
- During dNTP polymerization, it uses a combination of negative (N-1 and N-3) and positive (N-1 and N-6) selectivity to differentiate between right and wrong dNTPs, while the shape of the base pair is essentially irrelevant. PMID: 17209555
- These results argue that cells can tolerate low levels of p180 as long as Mcm10 is present to "recycle" it. PMID: 17699597
- And-1/Ctf4 is therefore a new replication initiation factor that brings together the MCM2-7 helicase and the DNA pol alpha-primase complex. PMID: 17761813
- Results show that depletion of DNA polymerase alpha and not Polepsilon or Poldelta by siRNA induces phosphorylation of Chk1 on Ser345, thus phenocopying antimetabolite exposure. PMID: 19177015
- DNA and p180 binding to an Mcm10 construct that contains both the ID and CTD, provide the first mechanistic insight into how Mcm10 might use a handoff mechanism to load and stabilize pol alpha within the replication fork. PMID: 19608746
顯示更多
收起更多
-
相關(guān)疾病：Pigmentary disorder, reticulate, with systemic manifestations, X-linked (PDR)
-
亞細(xì)胞定位：Nucleus. Cytoplasm, cytosol.
-
蛋白家族：DNA polymerase type-B family
-
數(shù)據(jù)庫鏈接：
Most popular with customers
-
-
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody
Applications: ELISA, WB, IHC
Species Reactivity: Human
-
-
-
-
-
-
