PMEL Antibody

中文名稱：

PMEL兔多克隆抗體
貨號：

CSB-PA021324LA01HU
規格：

￥440
圖片：
Western blot
All lanes: PMEL antibody at 14µg/ml + Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 71, 61, 67 kDa
Observed band size: 71 kDa

IHC image of CSB-PA021324LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA021324LA01HU diluted at 1:200 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) PMEL Polyclonal antibody
Uniprot No.：

P40967
基因名：

PMEL
別名：

95 kDa melanocyte specific secreted glycoprotein antibody; 95 kDa melanocyte-specific secreted glycoprotein antibody; D12S53E antibody; gp100 antibody; M-beta antibody; ME20 antibody; ME20 M/ME20 S antibody; ME20-M antibody; ME20-S antibody; ME20M antibody; ME20M/ME20S antibody; ME20S antibody; Melanocyte lineage specific antigen GP100 antibody; Melanocyte protein mel 17 antibody; Melanocyte protein Pmel 17 antibody; Melanocyte protein Pmel 17 precursor antibody; Melanocytes lineage-specific antigen GP100 antibody; Melanoma associated ME20 antigen antibody; Melanoma gp100 antibody; Melanoma-associated ME20 antigen antibody; Melanosomal matrix protein 17 antibody; Melanosomal matrix protein17 antibody; P1 antibody; p100 antibody; p26 antibody; PMEL 17 antibody; PMEL antibody; PMEL_HUMAN antibody; PMEL17 antibody; Premelanosome protein antibody; Secreted melanoma-associated ME20 antigen antibody; SI antibody; SIL antibody; SILV antibody; Silver (mouse homolog) like antibody; Silver homolog antibody; Silver locus protein homolog antibody; Silver, mouse, homolog of antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Melanocyte protein PMEL protein (25-467AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，PMEL Antibody (CSB-PA021324LA01HU)，的標記方式是Non-conjugated。對于PMEL Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA021324LB01HU	PMEL Antibody, HRP conjugated	ELISA
FITC	CSB-PA021324LC01HU	PMEL Antibody, FITC conjugated
Biotin	CSB-PA021324LD01HU	PMEL Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
基因功能參考文獻：
1. To provide a source of dual-specific T cells for ACT, we generated a transgenic mouse strain expressing a CAR specific for Her2 in leukocytes under the control of the vav promoter .This CAR mouse strain was bred onto the pMEL transgenic mouse strain, expressing a TCR specific for the premelanosome protein, gp100 PMID: 27965307
2. IMCgp100 (ImmTAC recognizing a peptide derived from the melanoma-specific protein, gp100, presented by HLA-A*0201) efficiently redirects and activates effector and memory cells from both CD8(+) and CD4(+) repertoires to kill melanoma cells. PMID: 28640942
3. our work attempts to provide structural insights into the RPT domain structure and to elucidate its contribution to Pmel17 amyloid fibril formation. PMID: 26754844
4. These findings help to clarify the mechanism of T-cell recognition of gp100 during melanoma responses and could direct the development of altered peptides for vaccination. PMID: 26917722
5. Data suggest that the Kringle-like domain of PMEL facilitates post-endoplasmic reticulum processing of disulfide-bonded PMEL dimers and promotes formation of PMEL functional amyloid fibrillar structures within multivesicular endosomes. PMID: 26694611
6. mutant N-terminally extended peptides exhibited significantly increased HLA-A*02:01 binding affinity and elicited CD8(+) T cell stimulation in vitro similar to the wtgp100209-217 epitope. PMID: 26507656
7. Data indicate that repeat domain (RPT) derived from Pmel17 aggregation kinetics were influenced only by lysolipid-containing phospholipid vesicles. PMID: 25451784
8. Melanosome-autonomous regulation of size and number: the OA1 receptor sustains PMEL expression. PMID: 24650003
9. SIL-TAL1 rearrangement identifies a distinct subtype with inferior outcome which could allow for individual therapeutic stratification for T-ALL patients. PMID: 24040098
10. the molecular basis for the distinct trafficking and morphogenetic properties of PMEL and GPNMB is the PKD domain PMID: 23452376
11. Data indicat that the N-terminal region of PMEL/Pmel17 is essential for the formation of melanosomal fibrils. PMID: 23389629
12. BACE2 cleaves the integral membrane form of PMEL within the juxtamembrane domain, releasing the PMEL luminal domain into endosomal precursors for the formation of amyloid fibrils and downstream melanosome morphogenesis. PMID: 23754390
13. The recombinant Pmel 17 plasmids were right as we expected by DNA sequencing PMID: 23643172
14. The most significant single-nucleotide polymorphism in the 12q13.2 locus is located immediately upstream of the promoter region of PMEL for vitiligo in Han Chinese. PMID: 22951725
15. An antibody drug conjugate reactive with PMEL17 exhibits target-dependent tumor cell killing in vitro and in vivo. PMID: 22613716
16. This is a review on two human amyloidogenic polypeptides, one associated with Parkinson's disease, alpha-synuclein (alpha-syn), and the other important for melanin synthesis, the repeat domain (RPT) from Pmel17.[Review] PMID: 21993592
17. MART-1- and gp100-expressing and -non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro. PMID: 21993558
18. Pmel17 repeat domain fibril dissolution is pH dependent PMID: 22092386
19. the DW and HoSi mutations alter PMEL TMD oligomerization and/or association with membranes, with consequent formation of aberrantly packed fibrils. PMEL mutations can model the conversion between physiological and pathological amyloid PMID: 21949659
20. the multistep processing of Pmel17 begins with an early cleavage during secretion that primes the protein for later functional processing. PMID: 21247888
21. Repeat domains of melanosome matrix protein Pmel17 orthologs form amyloid fibrils at the acidic melanosomal pH. PMID: 21148556
22. Data suggest that intramelanosomal pH regulates Pmel17 amyloid formation and its subsequent dissolution in vivo. PMID: 21106765
23. N-terminal region and the polycystic kidney disease-like domain is highly crucial for endoplasmic reticulum export, subcellular targeting, and fibril formation by Pmel17 and thus for establishing functional melanosomes. PMID: 20231267
24. Data conclude that sPmel17 is released by regulated proteolytic ectodomain shedding. PMID: 19884326
25. we identified the gp100/pMel17 melanosomal protein as the shared antigen recognized by three independent CD8+ CTL clones in HLA-A*6801-restricted fashion. PMID: 12135425
26. Data show that MITF appears to regulate the expression of the SILV and MLANA genes. PMID: 12819038
27. Antigen-specific T lymphocyte reactivity to gp100 peptides was seen in 15 of 17 (88%) vitiligo patients, with many demonstrating very high reactivity at levels comparable with those observed with common recall antigens PMID: 12925214
28. truncation of the repeat region within Pmel17 alters either fibrillogenic activity or the interaction of Pmel17 with melanin intermediates PMID: 14632201
29. CD8 T lymphocytes recognized a nonameric peptide on melanoma cells that comprises two noncontiguous segments of melanocytic glycoprotein gp100(PMEL17); the production of this peptide involves the excision of four amino acids and splicing of the fragments PMID: 15001714
30. PMEL17 is rapidly processed in the endoplasmic reticulum and glycosylated in the early Golgi PMID: 15096515
31. Data suggest that MART-1 is indispensable for Pmel17 function and thus plays an important role in regulating mammalian pigmentation. PMID: 15695812
32. Data show that the melanosomal protein Pmel17 is sorted into intralumenal vesicles by a mechanism that is dependent upon lumenal determinants and conserved in non-pigment cells. PMID: 16516837
33. the RPT domain of PMEL17/GP100 is essential for its function in generating the fibrillar matrix of melanosomes and that the luminal domain is necessary for its correct processing and trafficking to those organelles PMID: 16682408
34. Pmel17 is a critical component of melanogenesis. PMID: 16704461
35. These data reveal a dual sorting defect in a natural mutant of Pmel17 and support a requirement of endocytic trafficking in Pmel17 fibril formation. PMID: 16760433
36. addition of sialic acid affects the stability and sorting of Pmel17 and reduces pigmentation PMID: 17303571
37. GTP-bound form of Rab7 promotes melanogenesis through the regulation of gp100 maturation in melanoma cells. PMID: 17625594
38. Premelanosome amyloid-like fibrils are composed of only golgi-processed forms of Pmel17 that have been proteolytically processed in endosomes PMID: 17991747
39. Measurement of Melan-A, gp100, MAGE-3, MIA and tyrosinase represents a prognostic factor and a method for early detection of metastasis and treatment response of melanoma patients. PMID: 18181974
40. Tyrosinase-, gp100-, or TRP-2-specific CD8(+) T cells could not be identified in the peripheral blood of individuals with vitiligo. PMID: 18337837
41. Nuclear to non-nuclear Pmel17/gp100 expression (HMB45 staining) is a discriminator between benign and malignant melanocytic lesions. PMID: 18552823
42. MHC class II presentation of gp100 epitopes in melanoma cells requires the function of conventional endosomes and is influenced by melanosomes. PMID: 19017974
43. release of the Pmel17 ectodomain, which is critical for melanin amyloidogenesis, is initiated by S2 cleavage at a juxtamembrane position PMID: 19047044
44. the repeat domain of the melanosome fibril protein Pmel17 forms the amyloid core promoting melanin synthesis PMID: 19666488
45. N-terminal domains elicit formation of functional Pmel17 amyloid fibrils. PMID: 19840945
46. Details and validates the multiple sorting pathways used by the silver protein to reach melanosomes. It identifies the specific adaptor proteins involved and sheds light on the polarization of the melanocyte. PMID: 16492709
47. Detailed analysis of the post-translational modifications and their effect over the processing and trafficking of the silv gene. PMID: 17303571
48. The PMEL17 (SILV) protein catalyzes the polymerization of 5,6-dihydroxyindole-2-carboxylic acid to melanin. PMID: 8617263
49. Pmel17 is proteolytically processed in a post-Golgi compartment and is enriched in multivesicular endosomes prior to incorporation in stage II melanosomes. PMID: 11694580
50. A proteolytic fragment of Pmel17, generated by proprotein convertase cleavage, is the major biogenetic component of the underlying fibrillar matrix of melanosome precursors. PMID: 12732614
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亞細胞定位：

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus. Melanosome. Endosome, multivesicular body. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.; [M-alpha]: Secreted.
蛋白家族：

PMEL/NMB family
組織特異性：

Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and
數據庫鏈接：

HGNC: 10880

OMIM: 155550

KEGG: hsa:6490

STRING: 9606.ENSP00000402758

UniGene: Hs.95972

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