PEX5 Antibody

說明書

中文名稱：

PEX5兔多克隆抗體
貨號：

CSB-PA040128
規格：

￥1090
圖片：
Western Blot analysis of A549 cells using Peroxin 5 Polyclonal Antibody
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P50542
基因名：

PEX5
別名：

FLJ50634 antibody; FLJ50721 antibody; FLJ51948 antibody; PBD2A antibody; PBD2B antibody; Peroxin 5 antibody; Peroxin-5 antibody; Peroxisomal biogenesis factor 5 antibody; Peroxisomal C terminal targeting signal import receptor antibody; Peroxisomal C-terminal targeting signal import receptor antibody; Peroxisomal targeting signal 1 (SKL type) receptor antibody; Peroxisomal targeting signal 1 receptor antibody; Peroxisomal targeting signal import receptor antibody; Peroxisomal targeting signal receptor 1 antibody; Peroxisome receptor 1 antibody; pex5 antibody; PEX5_HUMAN antibody; PTS1 BP antibody; PTS1 receptor antibody; PTS1-BP antibody; PTS1R antibody; PXR1 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthesized peptide derived from the C-terminal region of Human Peroxin 5.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
ELISA	1:40000

Protocols：

Western Blotting (WB) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.
基因功能參考文獻：
1. these findings lend credit to the idea that inefficient catalase import, when coupled with the role of PEX5 as a redox-regulated import receptor, constitutes a cellular defense mechanism to combat oxidative insults of extra-peroxisomal origin. PMID: 28760655
2. TRIM37-mediated ubiquitylation stabilizes PEX5 and promotes peroxisomal matrix protein import, suggesting that mulibrey nanism is a new peroxisomal biogenesis disorder. PMID: 28724525
3. Data suggest that soluble/cytosolic PEX5 interacts with PEX14/PEX13 complex, a model for the docking/translocation module (DTM) of the peroxisomal matrix protein translocon; PEX14/PEX13 complex appears to function in peroxisomal membrane as large cavity into which cytosolic PEX5 can enter to release its cargo. (PEX = peroxisomal biogenesis factor) PMID: 28765278
4. data reveal subpopulations of peroxisomes showing only weak colocalization between PEX14 and PEX5 or PEX11 but at the same time a clear compartmentalized organization. This compartmentalization, which was less evident in cases of strong colocalization, indicates dynamic protein reorganization linked to changes occurring in the peroxisomes. PMID: 27311714
5. our data suggest that insertion of the trimeric PEX5-PEX7-PTS2 protein complex into the DTM is probably accompanied by conformational alterations in PEX5 to allow release of the PTS2 protein into the organelle matrix PMID: 26138649
6. Our data suggest that the functional polymorphism rs3814058C>T in 3'-UTR of PXR may be a functional biomarker to predict risk of colorectal cancer PMID: 26547791
7. PEX5 encodes two isoforms, PEX5L & PEX5S, & a homozygous frame shift mutation c.722dupA (p.Val242Glyfs( *)33), located in the PEX5L-specific exon 9, results in loss of PEX5L only. Loss of PEX5L results in deficient import of PTS2-tagged proteins PMID: 26220973
8. bulky side chain within the recognition motif, which blocks contraction of the PEX5 binding cavity PMID: 25369882
9. Data show that ataxia-telangiectasia mutated (ATM) phosphorylates peroxisomal biogenesis factor 5 (PEX5) at serine 141 in response to reactive oxygen species. PMID: 26344566
10. the molecular recognition of PTS1 cargo proteins by Pex5p PMID: 25689234
11. ubiquitination of peroxisome-targeting signal type 1 (PTS1) receptor Pex5p regulating PTS1 protein import PMID: 24662292
12. PEX5 has a role in regulating peroxisome numbers by signaling to mediate pexophagy PMID: 24453954
13. Cys11 in PEX5 serves as a functional redox switch regulating the peroxisomal/cytosolic localization of peroxisomal proteins. PMID: 24118911
14. The novel Pex14-binding site may represent the initial tethering site of Pex5 from which the cargo-loaded receptor is further processed in a sequential manner. PMID: 24235149
15. Translocation of proteins across the organelle membrane occurs downstream of a reversible docking step and upstream of the first cytosolic ATP-dependent step, i.e. before ubiquitination of PEX5. PMID: 23963456
16. The molecular mechanism of recognition by the peroxisomal receptor Pex5p, in complex with alanine-glyoxylate aminotransferase revealed by X-ray crystallography. PMID: 22529745
17. Our results suggest that ubiquitin-specific protease 9X (USP9X) is by far the most active deubiquitinase acting on Ub-PEX5, both in female rat liver and HeLa cells. PMID: 22371489
18. interaction of PEX5 with catalase and PEX14 PMID: 21976670
19. The Peroxisomal targeting signal 1 in Scp2 is autonomous and is essential for binding to pex5. PMID: 21375735
20. the affinities of the PTS1-containing peptides from 42 proteins from the human proteome for Pex5p PMID: 20178365
21. Data suggest that translocation of PTS1-containing proteins across the peroxisomal membrane occurs concomitantly with formation of the Pex5p-Pex14p membrane complex and that this is probably the site from which Pex5p leaves the peroxisomal compartment. PMID: 12411433
22. binds the PTS1 independently of Hsp70 and the peroxin PEX12 PMID: 12456682
23. Binding energy threshold data have been generated for PTS1 pentapeptides containing different C-terminal tripeptide sequences to determine the functionality of specific PTS1 sequences. PMID: 12578380
24. concluded that the PTS1 domain of SR-BI is functional and can mediate peroxisomal interaction via Pex5p, in vitro PMID: 14652019
25. the N terminus of Pex5p is required for redirecting the peroxisome-associated peroxin back to the cytosol PMID: 15328363
26. Pex5p is a monomeric protein with an abnormal shape PMID: 15866874
27. Pex5 most likely enters peroxisomes, changes its interacting partners, and then exits using ATP energy. PMID: 16314507
28. Results indicate that the N-terminal half of Pex5p is best described as a natively unfolded pre-molten globule-like domain. PMID: 16403517
29. study shows mutation-dependent, gain-of-function association between myocilin & PTS1R; there was correlation between glaucoma phenotype & specific MYOC mutations, with more severe early-onset POAG mutations having higher degree of association with PTS1R PMID: 17317787
30. Results describe the affinities of mutated forms of Pex5p for a series of peroxisomal targeting signal type-1 (PTS1) peptides and conclude that PTS1-affinity reductions are correlated with Zellweger disease severity and cell biological phenotype. PMID: 17399738
31. A previously unobserved conformation for PEX5 suggests roles for intrinsic flexibility and rigid domain motions in ligand binding. PMID: 17428317
32. E2D1/2/3 (UbcH5a/b/c) are the mammalian functional counterparts of yeast/plant Pex4p PMID: 18359941
33. The location of the different mutations within the PEX5 amino acid sequence correlates rather well with the peroxisomal protein import defect observed in the cell lines. PMID: 18712838
34. Results describe the properties of the soluble and membrane-bound monoubiquitinated Pex5p species (Ub-Pex5p). PMID: 19208625
35. analysis of the human Pex5.Pex14.PTS1 protein complex structure obtained by small angle X-ray scattering PMID: 19584060
36. the step of protein translocation across the peroxisomal membrane into the PEX5 cycling pathway PMID: 19632994
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相關疾病：

Peroxisome biogenesis disorder 2A (PBD2A); Peroxisome biogenesis disorder 2B (PBD2B); Rhizomelic chondrodysplasia punctata 5 (RCDP5)
亞細胞定位：

Cytoplasm. Peroxisome membrane; Peripheral membrane protein. Note=Its distribution appears to be dynamic. It is probably a cycling receptor found mainly in the cytoplasm and as well associated to the peroxisomal membrane through a docking factor (PEX13).
蛋白家族：

Peroxisomal targeting signal receptor family
組織特異性：

Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
數據庫鏈接：

HGNC: 9719

OMIM: 202370

KEGG: hsa:5830

STRING: 9606.ENSP00000407401

UniGene: Hs.567327

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