PEMT Antibody

中文名稱：

PEMT兔多克隆抗體
貨號：

CSB-PA017780LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate
All lanes: PEMT antibody at 2.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 23, 26, 25 kDa
Observed band size: 23 kDa

Immunofluorescent analysis of A549 cells using CSB-PA017780LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產品詳情
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靶點詳情

產品詳情

產品描述：

The PEMT antibody is a polyclonal antibody generated in the rabbit with the recombinant human Phosphatidylethanolamine N-methyltransferase protein (2-49aa) immunized. It is an unconjugated IgG. Its purity is over 95% using protein G affinity chromatography purified. This PEMT antibody has been quality tested in ELISA, WB, and IF applications. It shows reactivity with human PEMT protein.

PEMT mainly catalyzes the conversion of phosphatidylethanolamine (PE) to phosphatidylcholine (PC), which is essential for maintaining the balance between these lipids in cell membranes. PEMT also plays a crucial role in lipid metabolism, lipoprotein secretion, and inflammation, and it has been linked to several diseases, including NAFLD, Alzheimer's disease, cancer, and cardiovascular disease.
產品名稱：

Rabbit anti-Homo sapiens (Human) PEMT Polyclonal antibody
Uniprot No.：

Q9UBM1
基因名：

PEMT
別名：

PEMT; PEMPT; PNMT; Phosphatidylethanolamine N-methyltransferase; PEAMT; PEMT; PEMT2; Phospholipid methyltransferase; PLMT
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Phosphatidylethanolamine N-methyltransferase protein (2-49AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，PEMT Antibody (CSB-PA017780LA01HU)，的標記方式是Non-conjugated。對于PEMT Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA017780LB01HU	PEMT Antibody, HRP conjugated	ELISA
FITC	CSB-PA017780LC01HU	PEMT Antibody, FITC conjugated
Biotin	CSB-PA017780LD01HU	PEMT Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the three sequential steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME), PMME to phosphatidyldimethylethanolamine (PDME), and PDME to phosphatidylcholine (PC).
基因功能參考文獻：
1. Study found three gene variants (CLOCK-rs4864548, PEMT-rs936108, and GHRELIN-rs696217) that exhibited uncorrected gene-by-sleep duration interactions in relation to BMI z-scores in a cohort of New Zealand European children. However, no interactions were identified in percentage body fat differences. Notably, these interactions are evident without detectable effects on sleep duration. PMID: 28899534
2. In genotypic combination analysis considering PEMT -744GG/CHDH +432GG/BHMT +742GG as the reference combination, PEMT -744GC/CHDH +432GG/BHMT +742GG genotypic combination was significantly higher in mothers of a down syndrome child compared with that in control mothers with an odds ratio of 2.061 (95% CI: 1.10-3.86, P=0.0342). PMID: 27677362
3. Results showed that PEMT mRNA expression in liver tissues of non-alcoholic steatohepatitis (NASH) patients was significantly lower than those with simple steatosis suggesting a distinct clinical entity of lean NASH with insufficiency of PEMT activities. PMID: 26883167
4. a significant association between the PEMT rs7946 A-allele and a risk of nonalcoholic fatty liver disease, with the effect being more prominent in East-Asians, but not in non-Asians (Meta-Analysis) PMID: 26636496
5. Data show that phosphatidylethanolamineN-methyltransferase (PEMT) rs12325817 polymorphism only marginally changed the association value with academic achievement. PMID: 26728177
6. Data suggest that maternal dietary intake during lactation (here, choline intake exceeding dietary recommendations) can alter hepatic PEMT activity and increase choline content of breast milk. PMID: 26025328
7. MTHFR rs1801131 C allele and PEMT rs4646356 T allele were associated with a high risk of type 2 diabetes in Han Chinese. PMID: 25074646
8. The GG genotype of the PEMT G774C polymorphism, higher levels of serum homocysteine and lower levels of serum betaine are associated with an increased risk of microangiopathy in patients with diabetes. PMID: 23794489
9. PEMT is a functional single nucleotide polymorphism (rs7946) in PEMT with sporadic Alzheimer's disease risk in a Han Chinese population. PMID: 21881829
10. the PEMT -774G>C and CHDH +432G>T polymorphisms were associated with sperm concentration. This finding suggests a possible influence of these genes on sperm quality PMID: 22387881
11. genetic association studies on endometriosis in a population of women in Poland: Data suggest interaction between an SNP in PEMT (rs4244593) and an SNP in MTHFR (Ala222Val; rs1801133) in infertile women with some indication of endometriosis. PMID: 21429654
12. nvestigation of factors affecting liver PEMT activity: PEMT activity is lower in liver samples from women who are homozygous for an SNP in PEMT (rs12325817) PMID: 21411618
13. allele-specific ablation of estrogen receptor-DNA interaction in the PEMT locus prevents hormone-inducible PEMT expression, conferring risk of choline deficiency in women PMID: 21059658
14. Choline requirements for both women are increased by the genetic polymorphism rs12325817 in phosphatidylethanolamine-N-methyltransferase. PMID: 20861172
15. Total intake of choline and genotype can influence the concentrations of choline and its metabolites in the breast milk and blood of lactating women and thereby affect the amount of choline available to the developing infant. PMID: 20534746
16. The allele frequency of PEMT did not show a significant difference between normal control group and fatty liver patients (P=0.222). PMID: 19262398
17. an examination of the membrane topography of this enzyme PMID: 12431977
18. PEMT2 mRNA expression was inversely related to tumor proliferation and to histologic grade. PMID: 12931022
19. PEMT-mediated Hcy secretion correlated with the methyltransferase activity of the enzyme, independently of subcellular localization PMID: 15927961
20. Val175Met variant of PEMT could be a susceptibility candidate to nonalcoholic steatohepatitis (NASH), because it is more frequently observed in NASH patients and non-obese persons with Val175Met variant of PEMT are facilitated to develop NASH PMID: 17391797
21. Human PEMT genes have three unique transcription start sites, which are indicative of either multiple promoters and/or alternative splicing. PMID: 17456783
22. The present results suggest that the PEMT gene may contribute to the etiology of schizophrenia. PMID: 17720317
23. single nucleotide polymorphisms of choline-metabolizing genes, PEMT -774G>C (rs12325817) and CHDH +432G>T (rs12676), were found be related to breast cancer risk PMID: 18230680
24. These data suggest that polymorphisms in PEMT genes relevant to choline metabolism modulate parameters of choline status when folate intake is restricted. PMID: 19167960
25. in folate-deplete men, several factors with roles in 1-carbon metabolism interact with the MTHFR C677T genotype to affect plasma homocysteine PMID: 19211833
26. No association of the rs4646396 SNP in the PEMT locus with schizophrenia in a Chinese case-control sample. PMID: 19647326
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亞細胞定位：

[Isoform 1]: Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein.; [Isoform 2]: Endoplasmic reticulum membrane; Multi-pass membrane protein.
蛋白家族：

Class VI-like SAM-binding methyltransferase superfamily, PEMT/PEM2 methyltransferase family
數據庫鏈接：

HGNC: 8830

OMIM: 602391

KEGG: hsa:10400

STRING: 9606.ENSP00000255389

UniGene: Hs.714193

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