PDK4 Antibody

說明書

中文名稱：

PDK4兔多克隆抗體
貨號：

CSB-PA642637
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA642637(PDK4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA642637(PDK4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

Gel: 10%SDS-PAGE, Lysate: 60 μg, Lane: Mouse pancreas tissue, Primary antibody: CSB-PA642637(PDK4 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
其他：

產品詳情
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靶點詳情

產品詳情

Uniprot No.：

Q16654
基因名：

PDK4
別名：

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4 antibody; FLJ40832 antibody; mitochondrial antibody; Pdk4 antibody; PDK4_HUMAN antibody; Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial antibody; Pyruvate dehydrogenase kinase 4 antibody; Pyruvate dehydrogenase kinase isoenzyme 4 antibody; Pyruvate dehydrogenase kinase isoform 4 antibody; Pyruvate dehydrogenase kinase isozyme 4 antibody; Pyruvate dehydrogenase kinase isozyme 4 mitochondrial antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Synthetic peptide of Human PDK4
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:1000-1:5000
WB	1:500-1:2000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
基因功能參考文獻：
1. Results show that PDK4-specific 5'UTR DNA methylation is positively associated with eicosapentaenoic and arachidonic acid in adult males. PMID: 27181711
2. it was found that low expression of FAM210B was significantly correlated with decreased survival and enhanced metastasis in vivo and in vitro, and the loss of FAM210B led to an increased mitochondrial respiratory capacity and reduced glycolysis through the downregulation of pyruvate dehydrogenase kinase 4 (PDK4). PMID: 28594398
3. In conclusion, our data indicated that PDK4 potentially contributes to the hepatic steatosis in NASH via regulating several signaling pathway and PDK4 may be a new therapeutic strategy against NAFLD. PMID: 29128353
4. Low PDK4 expression is associated with lung tumorigenesis. PMID: 27641336
5. Increased PDK4 expression is associated with colon cancer. PMID: 28208156
6. We found that PDK4 gene and protein expression was significantly elevated in pulmonary arterial hypertension pericytes and correlated with reduced mitochondrial metabolism, higher rates of glycolysis, and hyperproliferation PMID: 27456128
7. Inhibition of CK2 increased the expression of metabolic regulators, PDK4 and AMPK along with the key cellular energy sensor CREB. PMID: 27001465
8. These findings indicate that the TGFbeta/PDK4 signaling axis plays an important role in the response of colorectal cancer to chemotherapy. PMID: 27330076
9. inhibition of PDK4 activity in Hepatocellular carcinoma cells increased cyclin E1, cyclin A2, and E2F1 proteins. PMID: 28003426
10. FXR may promote the proliferation of tumor cells and the hepatocytes in the process of liver regeneration by activating the PDK4-mediated metabolic reprogramming to generate glycolytic intermediates essential for rapid biomass generation, establishing a mechanistic link between cell proliferation and metabolic switch. PMID: 26728993
11. Combined speed endurance and endurance exercise amplify the exercise-induced PGC-1alpha and PDK4 mRNA response in trained human muscle. PMID: 27456910
12. High PDK4 expression is associated with hepatocellular cancer. PMID: 26506419
13. upregulation of PDK4 promotes vascular calcification by increasing osteogenic markers with no adverse effect on bone formation, demonstrating that PDK4 is a therapeutic target for vascular calcification. PMID: 26560812
14. Fatty acids induce the expression of PDK4 mRNA, which was increased in myotubes cultured from obese and T2DM donors. PMID: 25311062
15. Swiss PDB Viewer identifies NFkappaB subunit p100/p49 as a potential interacting partner for PDK4. Relationship between PDK4 and apoptosis is suggested. PMID: 25794976
16. ZBTB2 may increase cell proliferation by reprogramming glucose metabolic pathways to favor glycolysis by upregulating PDK4 expression via repression of RelA/p65 expression PMID: 25609694
17. Methylation increased in PDK4, IL1 B, IL6, and TNF promoters 12 months after gastric bypass. PMID: 24837562
18. PDK4 promotes tumorigenesis through activation of the CREB-RHEB-mTORC1 signaling cascade. PMID: 25164809
19. Data show that the orphan nuclear receptor estrogen related receptor gamma (ERRgamma) plays a critical role in hypoxia-mediated activation of pyruvate dehydrogenase kinase 4 (PDK4) gene expression. PMID: 23050013
20. mRNA of PGC-1alpha and PDK4 increases markedly in both type I and type II skeletal muscle after either continuous or interval exercise PMID: 21883960
21. PDK4 polymorphisms may not be associated with type 2 diabetes or metabolic syndrome. PMID: 22019269
22. The present structures demonstrate the flexible and dynamic aspects of PDK4 in the open conformation and provide a basis for the development of novel inhibitors targeting the nucleotide-binding pocket of PDK4. PMID: 21904029
23. PDK4 gene is downregulated in colic adenocarcinoma. PMID: 20715114
24. The expression of the pyruvate dehydrogenase kinase 4 was downregulated in the failing heart. PMID: 12145475
25. results support the concept that a reduced tissue availability of fatty acids consequent to a massive lipid malabsorption influences glucose metabolism acting through the regulation of pyruvate dehydrogenase kinase 4 PMID: 12582211
26. mRNA levels are increased in skeletal muscle during fasting PMID: 14966024
27. Expression of constitutively active PKB alpha abrogates dexamethasone stimulation of hPDK4 promoter activity. PMID: 15047604
28. Gene expression regulation is affected by retinoic acid and histone acetyation (review). PMID: 15581486
29. activation of FXR may suppress glycolysis and enhance oxidation of fatty acids via inactivation of the pyruvate dehydrogenase complex by increasing PDK4 expression PMID: 15721319
30. retinoic acids (RA) as well as Trichostatin A (TSA), an inhibitor of histone deacetylase (HDAC), regulate PDK4 gene expression PMID: 16757381
31. Accumulation of im lipids plays a more important role than impaired activation of Akt-mediated pathways in the regulation of muscle PDK4 gene expression in lipid-induced acute insulin-resistant states PMID: 17652214
32. PDK4 upregulation in adipocytes participates in the hypolipidemic effect of thiazolidinediones through modulation of glyceroneogenesis PMID: 18519799
33. PDK4 with bound ADP exists in equilibrium between the open and the closed conformations PMID: 18658136
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亞細胞定位：

Mitochondrion matrix.
蛋白家族：

PDK/BCKDK protein kinase family
組織特異性：

Ubiquitous; highest levels of expression in heart and skeletal muscle.
數據庫鏈接：

HGNC: 8812

OMIM: 602527

KEGG: hsa:5166

STRING: 9606.ENSP00000005178

UniGene: Hs.8364

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