NT5C2 Antibody

中文名稱：

NT5C2兔多克隆抗體
貨號(hào)：

CSB-PA23789A0Rb
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: Mouse liver tissue
All lanes: NT5C2 antibody at 3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65, 62 kDa
Observed band size: 65 kDa

Immunofluorescence staining of Hela cells with CSB-PA23789A0Rb at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

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產(chǎn)品評(píng)價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) NT5C2 Polyclonal antibody
Uniprot No.：

P49902
基因名：

NT5C2
別名：

5' nucleotidase (purine) cytosolic type B antibody; 5NTC_HUMAN antibody; cN II antibody; Cytosolic 5''-nucleotidase II antibody; Cytosolic IMP/GMP-specific 5'-nucleotidase antibody; Cytosolic purine 5' nucleotidase antibody; Cytosolic purine 5''-nucleotidase antibody; GMP antibody; NT5B antibody; nt5c2 antibody; NT5CP antibody; NUCLEOTIDASE; 5-PRIME; CYTOSOLIC II antibody; NUCLEOTIDASE; 5-PRIME; TYPE B antibody; PNT5 antibody; PURINE 5-PRIME-NUCLEOTIDASE antibody
宿主：

Rabbit
反應(yīng)種屬：

Human, Mouse
免疫原：

Recombinant Human Cytosolic purine 5\'-nucleotidase protein (487-553AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，NT5C2 Antibody (CSB-PA23789A0Rb)，的標(biāo)記方式是Non-conjugated。對于NT5C2 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA23789B0Rb	NT5C2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA23789C0Rb	NT5C2 Antibody, FITC conjugated
Biotin	CSB-PA23789D0Rb	NT5C2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
基因功能參考文獻(xiàn)：
1. NT5C2 mutations seem to have a broad clinical spectrum and should be sought in patients manifesting either as uncomplicated or complicated spastic paraplegia PMID: 28884889
2. Using data from a large-scale genome-wide association study of schizophrenia, study identified several potentially functional variants relating to miRNA function with our top finding being a schizophrenia protective allele that disrupts miR-206s binding to NT5C2 thus leading to increased expression of this gene. PMID: 27424800
3. acute lymphoblastic leukemia-specific mutations affect regulation of cN-II PMID: 27756303
4. this study implicates altered neural expression of BORCS7, AS3MT, and NT5C2 in susceptibility to schizophrenia arising from genetic variation at the chromosome 10q24 locus PMID: 27004590
5. NT5C2 variant rs11191580 conferred susceptibility to schizophrenia and affected the clinical symptoms of schizophrenia in a South Chinese Han population. PMID: 27901213
6. The aberrantly spliced NT5C2 showed substantial reduction in expression level in the in-vitro study, indicating marked instability of the mutant NT5C2 protein. PMID: 28327087
7. Leukemia Relapse-Associated Mutation of NT5C2 Gene is Rare in de Novo Acute Leukemias and Solid Tumors. PMID: 26259531
8. cN-II co-immunoprecipitated both with wild type Ipaf and its LRR domain after transfection with corresponding expression vectors, but not with Ipaf lacking the LRR domain. PMID: 25811392
9. Data indicate that type II cytosolic 5'-nucleotidase (cN-II) exerts a role in nucleotide and drug metabolism and regulating cell survival. PMID: 25857773
10. Four novel body mass index-associated loci near the KCNQ1(rs2237892), ALDH2/MYL2 (rs671, rs12229654), ITIH4 (rs2535633) and NT5C2 (rs11191580) genes are identified in East Asian-ancestry populations. PMID: 24861553
11. These results suggest that mutations in NT5C2 are associated with the outgrowth of drug-resistant clones in acute lymphoblastic leukemia PMID: 23377183
12. results highlight the prominent role of relapse-specific mutations in NT5C2 as a mechanism of resistance to 6-Mercaptopurine and a genetic driver of relapse in acute lymphoblastic leukemia PMID: 23377281
13. analysis of Drosophila and human 7-methyl GMP-specific nucleotidases PMID: 23223233
14. Polymorphisms in the CYP17A1 and NT5C2 genes influence a reduction in both visceral and subcutaneous fat mass in Japanese women. PMID: 22071413
15. seven high-resolution structures of human cN-II, including a ligand-free form and complexes with various substrates and effectors, were presented. PMID: 21396942
16. cN-II has a role in protecting against progression of non-small cell lung cancer PMID: 15923058
17. the expression level of cN-II mRNA might be a prognostic factor of high-risk MDS. PMID: 17350683
18. Data describe the crystal structure of human cytosolic 5'-nucleotidase II and discuss its allosteric regulation and substrate recognition. PMID: 17405878
19. In leukoblasts from 82 patients with acute myeloid leukemia, various extent and frequency of differential allelic expression in the CDA, DCK, NT5C2, NT5C3, and TP53 genes was observed. PMID: 18775979
20. The DCK/cN-II ratio was again proportional to ara-CTP production and to ara-C sensitivity. PMID: 19428333
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相關(guān)疾病：

Spastic paraplegia 45, autosomal recessive (SPG45)
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

5'(3')-deoxyribonucleotidase family
數(shù)據(jù)庫鏈接：

HGNC: 8022

OMIM: 600417

KEGG: hsa:22978

STRING: 9606.ENSP00000339479

UniGene: Hs.734531

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