NEK2 Antibody
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中文名稱：NEK2兔多克隆抗體
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貨號：CSB-PA015699ESR1HU
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規格：￥440
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圖片：
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA015699ESR1HU at dilution of 1:100
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) NEK2 Polyclonal antibody
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Uniprot No.：
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基因名：NEK2
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別名：HSPK 21 antibody; HsPK21 antibody; NEK 2 antibody; NEK2 antibody; NEK2_HUMAN antibody; NEK2A antibody; Never in mitosis A-related kinase 2 antibody; NIMA (never in mitosis gene a) related kinase 2 antibody; NimA like protein kinase 1 antibody; NIMA related kinase 2 antibody; NimA related protein kinase 2 antibody; NimA-like protein kinase 1 antibody; NimA-related protein kinase 2 antibody; NLK 1 antibody; NLK1 antibody; Serine/threonine-protein kinase Nek2 antibody
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宿主：Rabbit
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反應種屬：Human
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免疫原：Recombinant Human Serine/threonine-protein kinase Nek2 protein (1-135AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：Antigen Affinity Purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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產品提供形式：Liquid
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應用范圍：ELISA, IHC
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推薦稀釋比：
Application Recommended Dilution IHC 1:20-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Involved in the regulation of centrosome disjunction.; Phosphorylates and activates NEK11 in G1/S-arrested cells.; Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells.
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基因功能參考文獻：
- Taken together with the result that gamma-taxilin protein expression levels were decreased at the onset of mitosis, we propose that gamma-taxilin participates in Nek2A-mediated centrosome disjunction as a negative regulator through its interaction with Nek2A. PMID: 29225051
- NEK2 can promote HCC metastasis through induction of the epithelial-mesenchymal transition. PMID: 29399700
- evaluated the role of NEK2 in bone destruction of MM via clinical gene expression profiling data analysis and in vitro and in vivo mouse models. Further, treatment with the heparanase inhibitor Roneparstat, a Phase 1 drug in MM treatment,14 markedly inhibited OCL differentiation and rescued bone mass loss induced by NEK2 overexpression, suggesting that targeting NEK2 and/or its signaling pathway may be not only for preven PMID: 28400617
- Nek2 is able to individually shorten cilia when cilia are growing but requires interaction with Aurora A when cilia are being absorbed. PP1 binding protein, PPP1R42, directly inhibits Nek2 and increases cilia formation. PMID: 29141582
- Data suggest that low NIMA-related kinase 2 (NEK2) expression might be a useful predictor in hepatocellular carcinoma (HCC) as a poor prognostic factor, and could serve as a potential therapeutic target for HCC. PMID: 28759960
- present study firstly reveals that NEK2 protein is widely overexpressed in gliomas. NEK2 overexpression correlates significantly with malignancy (WHO grades), proliferation (Ki-67) and prognosis in malignant gliomas PMID: 28321704
- Results found NEK2 to be upregulated in lung cancer tissues and, its 3'-UTR mRNA is directly targeted by miR-128 to regulate its protein expression. PMID: 28514100
- Displacement of Dishevelled (DVL) from the centrosome and its release into the cytoplasm on NEK2 phosphorylation is coupled to the removal of linker proteins. PMID: 27486244
- NEK2 promotes aerobic glycolysis through regulating splicing of PKM and increasing the PKM2/PKM1 ratio in myeloma cells which contributes to its oncogenic activity. PMID: 28086949
- Nek2A is found to stabilize SuFu at least partly depending on its kinase activity, thereby triggering phosphorylation of the SuFu protein. PMID: 27297360
- NEK2 has a role in maintaining glioma stem cells in glioblastoma by stabilizing the EZH2 protein PMID: 28737508
- This study reports a novel set of Dengue virus (DENV) NS1-interacting host cell proteins in the HepG2 cell line and proposes possible roles for human NEK2, TAO1, and COG1 in DENV infection. PMID: 27108190
- Our findings suggested that NIMA-related kinase 2B could play a role in the development and progression of breast cancer. Combination treatment using NIMA-related kinase 2B small interfering RNA and paclitaxel might be a novel potential therapy method for breast cancer. PMID: 28475000
- NEK2 modulates hepatoma cell functions, including growth, drug resistance, metastasis and angiogenesis via downstream genes activation PMID: 27925179
- Alteration of NEK2 protein levels may contribute to invasion and metastasis of hepatocellular carcinoma PMID: 28101574
- the functional roles of NEK2 in cancer development (review) PMID: 27019372
- NEK2 was associated with unfavorable outcomes in HCC patients. PMID: 27349376
- enhanced NEK2 expression in hepatocellular carcinoma (HCC) promotes HCC progression and drug resistance by promoting PP1/Akt and Wnt pathway activation, which may represent a new therapeutic target for HCC. PMID: 27509921
- Our findings demonstrated that NEK2 could be a valuable carcinogenic factor and a promising therapeutic target for primary liver cancer PMID: 26337275
- Suggest elevated Nek2 protein expression may be an independent risk factor for colon cancer. PMID: 26823916
- Hepatocellular carcinoma patients with high NEK2 expression had significantly poor recurrence-free survival (p=0.042) and early recurrence after hepatectomy. PMID: 26851035
- Cep85 is a bona fide Nek2A-binding partner that surrounds the proximal ends of centrioles where it cooperates with PP1gamma (also known as PPP1CC) to antagonize Nek2A PMID: 26220856
- Kif24 is a physiological substrate of Nek2, which regulates cilia disassembly through a concerted mechanism involving Kif24-mediated microtubule depolymerization. PMID: 26290419
- The results suggest that overexpression of NEK2 has the potential to serve as a biomarker for PCa prognosis. PMID: 26320076
- Nek2A is degraded in mitosis regardless of enforced spindle checkpoint activation PMID: 25673878
- we highlight recent findings on the aberrant expression and functional significance of NEK2A in human cancers and emphasize their significance for therapeutic potentials. PMID: 25705694
- Findings are consistent with the hypothesis that NEK2 contributes to ALDH1A1-dependent drug resistance in myeloma PMID: 25230277
- Nek2 was demonstrated to phosphorylate Cep68 in vivo and this phosphorylation appears to promote Cep68 degradation in mitosis. PMID: 25704143
- Nek2 plays a central role in proteasome-mediated cell cycle regulation and in conferring resistance to bortezomib in cancer cells. PMID: 25313354
- High expression of Nek2 could serve as an independent predictor of poor prognosis in pancreatic ductal adenocarcinoma. PMID: 24966957
- Overexpression of the Nek2 kinase is associated with colorectal cancer. PMID: 25043295
- Data suggest a role for Nek2 driving centrosome amplification downstream of the E2F3 activator. PMID: 24797070
- These results identify a novel mechanism for regulating beta-catenin stability that is independent of GSK3beta and provide new insight into a pathway involving Plk1, Nek2, and beta-catenin that regulates the centrosome cycle. PMID: 24501426
- Nek2 may represent a new potential target for non-small cell lung cancer therapeutic intervention PMID: 24763826
- it appears that Nek2 and Plk4 might synergize to promote breast tumorigenesis and may also be involved in tamoxifen and trastuzumab resistance PMID: 24389189
- NEK2 directly or indirectly interacts with a number of genes, proteins, microRNAs and biological processes associated with drug resistance in ovarian and other types of cancer. PMID: 24337664
- Up-regulation of NEK2 by microRNA-128 methylation is associated with colorectal cancer. PMID: 24046120
- Nek2 overexpression could not induce centrosome amplification and chromosome instability without expression of TRF1. PMID: 24091727
- these data indicate that Nek2 has a pivotal role in breast cancer growth at primary and secondary sites, and thus may be an attractive and novel therapeutic target for this disease. PMID: 23708664
- CIP2A strongly interacts with NEK2 during G2/M phase, thereby enhancing NEK2 kinase activity to facilitate centrosome separation in a PP1- and PP2A-independent manner. PMID: 24214971
- NEK2 as a novel p53-repressed gene PMID: 24163369
- These results suggest that the malignant transformation of plexiform neurofibroma is associated with distinct changes in the expression of BUB1B, PBK and NEK2 PMID: 23370767
- Data conclude that centrosome amplification is modulated through Cdk4 and Nek2 signaling and that binucleation is a likely source of CA in Her2+ breast cancer cells. PMID: 23776583
- Whole genome sequencing in patients with retinitis pigmentosa reveals pathogenic DNA structural changes and NEK2 as a new disease gene. PMID: 24043777
- NEK2 phosphorylation antagonizes the microtubule stabilizing activity of centrobin. PMID: 23291182
- small interfering RNA and antisense oligonucleotides against Nek2 worked synergistically with paclitaxel and doxorubicin by promoting cell apoptosis. PMID: 23340795
- The recruitment of Nek2 to the proximal ends of centrioles is dependent on one of its substrates, the centrosome cohesion protein C-Nap1. PMID: 22613497
- Our results indicate that genetic polymorphisms of centrobin and Nek2 are related to breast cancer susceptibility in Chinese Han women. PMID: 23001753
- Nek2 C downregulation, using RNA interference, decreased the survival, invasion and migration of MCF10DCIS.com and MCF10CA1a cells as well as in human primary breast cancer tissue. PMID: 22824957
- Nek2A binds with high affinity to apo-APC/C and is degraded by the pool of Cdc20 that avoids inhibition by the spindle assembly checkpoint. PMID: 23288039
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相關疾病：Retinitis pigmentosa 67 (RP67)
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亞細胞定位：[Isoform 1]: Nucleus. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere. Note=STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGO1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.; [Isoform 2]: Cytoplasm. Note=Predominantly cytoplasmic.; [Isoform 4]: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly nuclear.
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蛋白家族：Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
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組織特異性：Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors.
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