NDFIP1 Antibody

中文名稱：

NDFIP1兔多克隆抗體
貨號：

CSB-PA887119LA01HU
規(guī)格：

￥440
圖片：
Western blot
All lanes: NDFIP1 antibody at 8µg/ml
Lane 1: 293T whole cell lysate
Lane 2: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 25, 17 kDa
Observed band size: 25 kDa

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA887119LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA887119LA01HU at dilution of 1:100

Immunofluorescent analysis of 293 cells using CSB-PA887119LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CUSABIO uses a partial recombinant protein mapping within 2-116 amino acids of human NDFIP1 protein to immunize the rabbit to obtain the NDFIP1 polyclonal antibody. This antibody exists as the unconjugated IgG isoform. Its purity is 95%+ using protein G purified. And it is only reactive to human samples. It has been validated for use in ELISA, WB, IHC, and IF. Its target protein NDFIP1 plays a neuroprotective role through mediating ubiquitination of target proteins in neuronal injury.
產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) NDFIP1 Polyclonal antibody
Uniprot No.：

Q9BT67
基因名：

NDFIP1
別名：

NDFIP1; N4WBP5; PSEC0192; PSEC0223; NEDD4 family-interacting protein 1; Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative NFKB and MAPK-activating protein
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human NEDD4 family-interacting protein 1 protein (2-116AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產(chǎn)品，NDFIP1 Antibody (CSB-PA887119LA01HU)，的標記方式是Non-conjugated。對于NDFIP1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產(chǎn)品名稱	應用
HRP	CSB-PA887119LB01HU	NDFIP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA887119LC01HU	NDFIP1 Antibody, FITC conjugated
Biotin	CSB-PA887119LD01HU	NDFIP1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cell-mediated inflammation by activating ITCH and thus controlling JUNB degradation. Promotes pancreatic beta cell death through degradation of JUNB and inhibition of the unfolded protein response, leading to reduction of insulin secretion. Restricts the production of proinflammatory cytokines in effector Th17 T-cells by promoting ITCH-mediated ubiquitination and degradation of RORC. Together with NDFIP2, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination. Regulates peripheral T-cell tolerance to self and foreign antigens, forcing the exit of naive CD4+ T-cells from the cell cycle before they become effector T-cells. Negatively regulates RLR-mediated antiviral response by promoting SMURF1-mediated ubiquitination and subsequent degradation of MAVS. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus where it mediates KCNH2 degradation. In cortical neurons, mediates the ubiquitination of the divalent metal transporter SLC11A2/DMT1 by NEDD4L, leading to its down-regulation and protection of the cells from cobalt and iron toxicity. Important for normal development of dendrites and dendritic spines in cortex. Enhances the ubiquitination of BRAT1 mediated by: NEDD4, NEDD4L and ITCH and is required for the nuclear localization of ubiquitinated BRAT1. Enhances the ITCH-mediated ubiquitination of MAP3K7 by recruiting E2 ubiquitin-conjugating enzyme UBE2L3 to ITCH. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. Inhibits cell proliferation by promoting PTEN nuclear localization and changing its signaling specificity.
基因功能參考文獻：
1. we identified Nedd4-family interacting protein 1 as a direct target of miR-155, and the expression of Nedd4-family interacting protein 1 was inhibited by miR-155. Furthermore, ectopic expression of Nedd4-family interacting protein 1 restored the effects of miR-155 on cell proliferation and invasion of uveal melanoma cells PMID: 29333944
2. the expression of Ndfipl reduced expression of a-synuclein. In conclusion, Ndfipl plays a significant role in protecting SH-SY5Y cells in in vitro Parkinson's disease models. PMID: 27173227
3. Silencing of Ndfip1 inhibited cytokine-induced apoptosis of pancreatic islets and promoted glucose-stimulated insulin secretion. These effects were associated with an increase in the cellular content of JunB, a potent inhibitor of ER stress and apoptosis. PMID: 26319551
4. Ndfip1 is required during stress for ubiquitinating and trafficking BRAT1 into the nucleus. PMID: 25631046
5. In Parkinson's disease, increased iron levels are associated with increased Ndfip1 expression for the regulation of DMT1, including abnormal Ndfip1 activation in non-neuronal cell types such as astrocytes. PMID: 24475238
6. Cellular up-regulation of Nedd4 family interacting protein 1 (Ndfip1) using low levels of bioactive cobalt complexes. PMID: 21187286
7. Ndfip1 is required for the exosomal secretion of Nedd4 family proteins PMID: 18819914
8. Ndfip1 plays a critical role in regulating metal transport in human neurons through its regulation of DMT1. PMID: 19706893
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亞細胞定位：

Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane. Cell junction, synapse, synaptosome. Cell projection, dendrite. Secreted. Note=Detected in exosomes and secreted via the exosomal pathway (PubMed:18819914).
組織特異性：

Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta. Also expressed in fetal brain, kidney and lung.
數(shù)據(jù)庫鏈接：

HGNC: 17592

OMIM: 612050

KEGG: hsa:80762

STRING: 9606.ENSP00000253814

UniGene: Hs.653510

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